University Engineering
Resume:
*** **** *** * ** EET N EWAR K, D E *****
PHONE 240-***-**** FAX 302-***-**** E-MAI L GI *****@****.***
WEBSI TE: HTTP:// UD EL.ED U/ ~GI LLES C
CHRISTOPHER GILLESPIE
O B JEC TIVE
Seeking a research oriented position in the pharmaceutical or biotechnology industries
E DUC A TION
2003-2009 (Spring expected) Ph.D. Candidate Chemical Engineering, University of Delaware
2000-2003 B.S. Cum Laude Chemical Engineering, University of Maryland Baltimore County
D OC TOR AL R ES EAR C H
Advisors: Abraham M. Lenhoff & Eric W. Kaler
Thesis: Protein solution thermodynamics: Polymorphic crystal growth, liquid-liquid separation and
predictive protein-protein interactions
Experimental techniques:
Vapor diffusion & microbatch crystallization, static light scattering, confocal laser scanning fluorescence
microscopy, protein chromatography, UV-Vis spectroscopy, self-interaction chromatography, Trp-
fluorescence, SDS-PAGE, Ultrafiltration
Software/Languages:
Fortran, Shell scripting (Unix), Matlab, Python, Tcl/Tk, OpenMP (parallelization), Unicorn ( kta),
NAMD (molecular dynamics), Gaussian, PyMol, VMD, POV-Ray, Swiss-PDB Viewer, APBS, Microsoft
Office, Keynote, DeltaGraph
W ORK EXPERIENC E
7/2001-7/2003 WR Grace/UMBC Baltimore, MD
I n t e rn I mmu n o ads o rbe n t s G ro u p
Performed binding capacity studies on new Protein A column packing material for antibody
purification
Performed breakthrough curves on new Protein A column packing material
Performed mass transfer calculations to relate diffusion through pores to theory
Studied the leaching of Protein A from the column packing material using an ELISA kit
7/1999-7/2001 Cambrex BioScience Baltimore, MD
P ro c e s s De ve l o pme n t Te c h n ic i an
Developed both purification and microbial fermentation processes for eventual cGMP production
Performed protein purification in columns ranging from
Supervised personnel in cGMP phase 1 purification
Developed process from inclusion bodies through refolding to ultimate >95% purity
Wrote, reviewed and edited batch records, SOPs, and SMPs for cGMP production
Trained new and existing employees in procedures and equipment use
Assisted in the development of a new purification procedure for the purification of plasmid DNA
H ON ORS & A WAR DS
University of Delaware Graduate Scholars Award, UD 2004, 2005, 2007, 2008
Alfred P. Sloan Minority Scholarship, UD 2003
University of Delaware Presidential Award, UD 2003
Chemistry/Biology Interface Program, UD 2003-Present
Outstanding Senior in Chemical Engineering, UMBC May 2003
McNair Scholar, UMBC 2003
Phi Beta Kappa, UMBC 2003
Golden Key International Honor Society, UMBC 2002
P UBLIC A TIONS & PRESE NTATIONS
Papers:
1. Mike Sleutel, Ronnie Willaert, Christopher Gillespie, Christine Evrard, Lode Wyns and Dominique
Maes, Kinetics and thermodynamics of glucose isomerase crystallization, Crystal Growth & Design (Accepted)
2. Christopher M. Gillespie, Dilipkumar Asthagiri, Eric W. Kaler and Abraham M. Lenhoff, Polymorphic
protein crystal growth: Influence of hydration and ions in glucose isomerase, In Preparation
Presentations:
1. Christopher M. Gillespie, Dilipkumar Asthagiri, Eric W. Kaler and Abraham M. Lenhoff, Polymorphic
protein crystal growth: The role of hydration in glucose isomerase crystal stability, AIChE Annual meeting,
Philadelphia, Pennsylvania, 2008
2. Christopher M. Gillespie, Eric W. Kaler and Abraham M. Lenhoff, Experimental and modeling investigation of
polymorphic crystal growth of glucose isomerase, Chemistry/Biology Interface, University of Delaware, March
2008
3. Christopher M. Gillespie, Eric W. Kaler and Abraham M. Lenhoff, Stability of polymorphic protein crystals of
glucose isomerase, Chemistry/Biology Interface, University of Delaware, March 2007
4. Christopher M. Gillespie, Eric W. Kaler and Abraham M. Lenhoff, Stability of polymorphic protein crystals of
glucose isomerase, University of Delaware, January 2007
5. Christopher M. Gillespie, Eric W. Kaler and Abraham M. Lenhoff, Polymorphic crystal growth of glucose
isomerase, Chemistry/Biology Interface, University of Delaware, March 2006
6. Christopher M. Gillespie, E ric W. Kaler and Abraham M. Lenhoff, Phase diagrams and polymorphic crystal
growth of glucose isomerase, University of Delaware, June 2005
7. Christopher M. Gillespie, John J. Langford and Abraham M. Lenhoff, Efficient measurement of equilibrium
protein adsorption isotherms: S ingle and multi-component, Chemistry/Biology Interface, University of Delaware,
May 2004
8. Christopher M. Gillespie, Todd Epstein and Brian Bahnson, Molecular refinement of the crystal structure of
platelet-activating factor acetylhydrolase with p-succinylamidephenyl arsenoxide, Chemistry/Biology Interface,
University of Delaware, March 2004
9. Christopher M. Gillespie, Erin Bernberg and Joan Burnside, Comparison of host gene expression between chicken
embryo fibroblasts infected with the wild type and LAT mutant of Marek's Disease virus using DNA microarrays,
Chemistry/Biology Interface, University of Delaware, February 2004
Posters:
1. Christopher M. Gillespie, Dilipkumar Asthagiri, Eric W. Kaler and Abraham M. Lenhoff, Polymorphic
crystal growth: The role of hydration in glucose isomerase crystal stability, ACS National meeting, Physical
Chemistry division, Philadelphia, Pennsylvania, 2008
2. Christopher M. Gillespie, Dilipkumar Asthagiri, Eric W. Kaler, Abraham M. Lenhoff, Polymorphic protein
crystal growth: An experimental and modeling investigation of glucose isomerase, Physical Chemistry division,
ICCBM12, Cancun, Mexico, 2008
3. Christopher M. Gillespie, Dilipkumar Asthagiri, Eric W. Kaler, Abraham M. Lenhoff, Polymorphic protein
crystal growth: An experimental and modeling investigation of glucose isomerase, Frontiers of the Chemistry Biology
Interface, University of Maryland College Park, 2008
4. Natraj Ram, Christopher Gillespie, Donald Durham, L ab-scale purification of high-quality plasmid DNA using
hydrophobic interaction chromatography, 3rd Annual Meeting Amer. Soc. Gene Therapy, 2000
References available upon request
Contact this candidate