Engineering Quality Control

Stella Alimperti ****

Stella Alimperti

Work: Home:

University at Buffalo 761 Wright Ave

Chemical & Biological Engineering Department North Tonawanda, NY, 14120

*** ****** **** ***

Amherst, NY 14260

USA

work: +1-716-9511

e-mail: *****@*******.***

Personal Statement

I am a friendly, loyal and clearly dedicated individual who has an ambition to s ucceed in any given

environment. I love to learn, and I a m always up for new challenge.

Research/ Working Experience

Jul 2011 Present: Collaborator: Life Technologies, Invitrogen, Grand Island, United States.

Project: Development of a Novel Culture System to Expand Mesenchymal Stem Cells in Suspension as

Spheroids.

Sep 2006- May 2007: Researcher/ Quality Control Analyst: Pillsbury, General Mills, Oinofyta,

Greece. Project: Quality analysis of products during process .

May 2005-Sep 2005: Researcher/ Chemical Analyst: Bundesanstalt f r Materialforschung und

pr fung, BAM, Berlin, Germany. Project: Analysis of gas purity by Atomic Absorption

spectroscopy (AAS).

Education

Aug 2007 present: Doctoral Research in progress, Research group of Prof. Stelios Andreadis ;

Chemical & Biological Engineering Department, University at Buffalo, Amherst, NY, USA

Doctor of Philosophy (Overall GPA: 3.5/4.00).

Thesis: Regulation of gene expression during myogenic differentiation.

Oct 2001 Jul 2006: Diploma, School of Chemical Engineering, National Technical University of

Athens (NTUA), Athens, Greece

Diploma in Chemical Engineering

Graduated 5% among class of 2006, (Overall GPA: 8.31/10.00).

Diploma Thesis: Computational analysis of chemotaxis models in biological systems.

Stella Alimperti 2012

Publications:

Tian, J., Alimperti, S., Lei, P., and Andreadis, S.T. (2010) Lentiviral microarrays for real-time

monitoring of gene expression dynamics. Lab Chip. 10(15): p. 1967-75.

Alimperti, S., Lei, P., Tian, J., and Andreadis, S.T. (2012). A novel lentivirus for quantitative

assessment of gene knockdown in stem cell differentiation. Gene Therapy.

Alimperti, S., Andreadis, S.T (submitted in Stem Cells). OB-cadherin is a master regulator of

cell-cell contact induced myogenic differentiation of human mesenchymal stem cells.

Alimperti, S., Campbell,A., Wen Y., Lei P.,Andreadis, S.T and Gorfien S. (in preparation).

Serum-Free Spheroid Suspension Culture of Mesenchymal Stem Cells.

Oral Presentations-Posters:

Tian, J., Alimperti, S., Lei, P., and Andreadis, S.T. Lentiviral microa rrays for real-time

monitoring of gene expression dynamics. Biomedical Engineering Society, Annual Meeting,

Pittsburgh, October, 2009.

Alimperti, S., Lei, P., Tian, J., and Andreadis, S.T. Quantitative Assessment of Cell Signaling

Pathways Affecting Stem Cell Differentiation Using Lentiviral Arrays. Biomedical Engineering

Society, Annual Meeting, Hartford, October, 2011.

Alimperti, S., Lei, P., Tian, J., and Andreadis, S.T. Quantitative Assessment of Cell Signaling

Pathways Affecting Stem Cell Differentia tion Using Lentiviral Arrays. American Institute of

Chemical Engineers, Annual Meeting, November, 2011

Alimperti, S., Andreadis, S.T . Regulation of Mesenchymal Stem Cell Myogenic Differentiation

by Cell-Cell Adhesion: The Role of Cadherins In Differentia tion. American Institute of Chemical

Engineers, Annual Meeting, November, 2011.

Alimperti, S., Campbell, A., Wen Y., Lei P., Andreadis, S.T and Gorfien S. Serum-Free Spheroid

Suspension Culture of Mesenchymal Stem Cells. International Society for Cellular Therapy, June,

2012.

Skills

Experimental skills:

Cell culture: Stem cells (BM-MSCs, HF-MSCs), MSCs spheroids, isolation of keratinocytes and

Hair Follicle-MSCs)

Cloning: PCR/ siRNA

Virus production

Western Blot, mRNA isolation, ELISA.

Viral arrays/Microarrays

Computional Skills:

Building mathematical models for gene networks

Fortran

Matlab

Comsol-Femlab

Excel

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