Project Manager

Resume of

Damodara Rao.G

Carrier Objective:

Have +8 years of Research Experience in biopharmaceutical Industries.

Hardworking, fast grasping & quick delivering of committed work with good

organizational skills, creative and productive too, largely self-directed

and able to work both independently and as a team member.

Professional Experience:

From To Designation Address

2012 Till Database Research Volunteer Ovarian Cancer Canada,

Nov Date Vancouver,BC

www.ovariancanada.org

Search, Maintain and update of Researcher's & Nurse databases.

Maintaining the hospital & Research Institutional databases.

From To Designation Address

2011 Till Biology Tutor Basran Avenue, New

July Date Westminster, BC, Canada

Teaching Biology and Chemistry for High school & College

students.

From To Designation Address

2012 2012 Inventory Associate JM Food Services, Burnaby,

Sep Nov (Contract) Canada.

Literature search and identified few better Ingredients &

different yeast varieties which are better for the food

production, cost effective, quite safe, and results in process

development.

Provide direction and support to production colleagues as

necessary.

High level of organizational skills in all aspects of the

Production and process development.

Maintain safety standards and perform safety audits.

Weekly perform cycle counts and update the Inventory, shipping,

receiving and packing

Regular check-up of MSDS, SOP's and regular participation of

Meetings and Audits.

Medium-Fast Typing and strong Knowledge in using MS Office.

Able to quickly learn new software packages.

From To Designation Address

2012 2012 Inventory Clerk (On Contract Honeywell, North Vancouver (

May Sep ) Employee of Cortech)

www.honeywell.com

Using Oracle software for stock update and Inventory

management, Cycle Count, Order Picking, Put away, Sorting,

Packing, Sales orders, Documentation with data entry.

Possess knowledge of various tasks involved in inventory

management.

Handled key inventory tasks and monitored for inventory

discrepancies

Reviewed existing inventory control procedures and revised them

wherever required

Prepared the Inventory Control and highlighted low stock

Preparation of Kaizans, Safety Observations (SO) for every

month.

Regular check-up of MSDS, SOP's and regular participation of

Meetings and Audits.

Trained new personnel on inventory flow and inventory data

recording procedures.

Fast Typing, Knowledge in using Oracle, word and excel. Able to

quickly learn new software packages

From To Designation Address

2011 2012 Inventory Clerk Ingram Micro (Fortune 100

Aug April Company)

Inventory, Cycle Counts, Checking the orders for Shipped and

received materials, computer hardware & Manufacturing hardware.

Dispatching drivers to customer's home and store locations.

Work with MS Office and Oracle software.

From To Designation Address

2008 2011 Research Scientist Panacea Biotec Private

Sep June Limited, India.

www.panaceabiotec.com.

Main Duties Primer Designing, Cloning, Expression and Molecular level Gene

Characterization of Recombinant Protein of therapeutic

Importance (Tumor Necrosis factor Receptor II / Etanercept).

Involved in the development of Single Vector and Double Vector

strategies.

Performed Receptor Binding studies and screening of drug

compounds using radio labeled 5HT6 (Serotonin receptor). Done

both Saturation and Competitive binding studies.

Molecular level Characterization of Recombinant Blood clotting

Factor VIII & Von Willebrand factor (vWF).

Identification of Expression Enhancers & Construction of

Recombinant Expression vector with the addition of Enhancer

Elements for the Increase of protein yield of the therapeutic

molecule.

Optimization of ELISA for the quantification of Bio molecules

using different ELISA Readers. Performed Direct, Indirect &

Sandwich ELISA for the quantification of Bio-similar's &

Therapeutic proteins of High Importance.

Expertise in Mammalian Cell Culture involves in Maintenance,

Transfection (Lipofectamine, Dreamfect & Freestyle max

reagents) Selection of Clones by drug, Kill curve Generation of

drug towards CHO Cell lines, Optimization of Antibiotic

Concentration /Amplification of Gene, Single Cell Dilution

Cloning, optimization of serum free adaptation process using

different media &performed Serum free adaptation of cells/yield

Checking and finally done Scale up from Flask to lab scale

Fermenter Level of Bio-similar or follow on biologics.

Small-scale Therapeutic protein production from Flask to

spinner level, Involved in the Molecular level

characterization.

Responsible for executing scientific research and development

strategies for the company.

Investigating concepts, planning laboratory research,

spokesperson on corporate research, interacting with various

departments and supporting all regulations.

From To Occupation Address

2007 2008 Research officer Glenmark pharmaceuticals.

July Sep www.glenmarkpharma.com

Senior Research Fellow II Avesthagen Technologies,

2007 India.

2005 July www.avesthagen.com

June

Main Duties Generation of ScFv Phage Display of Proteins of Therapeutic

Importance.

Involved as a lab member in the Establishment of molecular

biology lab which includes right from the start ordering of

chemicals, Discussions with the vendors, performing lab work &

regular maintenance of the lab Instruments.

Performed the Partial Purification & Lyophilization of

Recombinant molecules of therapeutic Importance (Tumor Necrosis

factor Receptor II).

Optimization of ELISA & Performed Direct, Indirect and Sandwich

ELISA for Quantification of Bio-similar Molecules.

Involved In the purification of Tumor Necrosis factor Receptor

II that got expressed & interacted with the team for the process

development of the molecule for better increase of yield.

Worked with various filtration systems (TFF systems of Millipore

& Pall Life Sciences) for the purification of the Molecule.

Performed the lyophilization cycle optimization (finding out the

Eutectic point) and Involved in the Formulation and Solid

Lyophilization of Tumor Necrosis factor Receptor II.

Lyophilization is done using Lyolab Lyophilized.

Attended Various Seminars Inside the Company & other places on

various Molecular biology & Protein Purification process areas.

Maintenance of Experimental observations, compile and use to

summarize complex and large data sets and use to prepare

reports, and presentations at group & project Meetings.

Done Standardization and Maintenance of Standard Operating

Procedures (SOP) of Equipments, Experiments, Regular Work Data

Sheets and Presentations.

From To Occupation Address

2002 2006 Research Associate Magene Life Sciences Private

Sep May Limited.

www.magenelifesciences.com

Main Duties Preparation of Buffers, Cell culture Media, plasmid Isolations

(Mini, Midi & Maxi kits).

Assisted the Senior Scientists in the Cloning and protein

purifications and routinely use to perform (Agarose gel, SDS

PAGE, DNA & Protein Quantification and checking the purity by

Nanodrop and U.V Spectrophotometer).

Got trained on various Molecular biology works on Bacterial &

Mammalian cells.

Involved in the Cloning of Biomolecule of Therapeutic Importance

(Dipeptidyl peptidase DPP-9 & DPP-8).

Got trained on the Chromatographic systems & worked on the

chromatographic Systems (Akta Prime & Akta Purifier - Amersham).

Trained on various Chromatographic Apparatus (Akta Purifier &

Akta Explorer) and Involved in the purification process as per

the GLP & GMP Guide lines.

Collection of Literature on Molecular Biology aspects of the

Molecule & use to take into consideration for the development of

the project.

Attended Various Seminars Inside the Company & other places on

various Purification process.

Trained on preparation of SOP's for Experiments & for Apparatus

for the Molecular Biology lab.

From To Training Address

2002 2002 Project Trainee -2 Post Graduate Institute of

May July Medical Education &

Research. (PGIMER),

Chandigarh, India

http://pgimer.nic.in/

Project Invitro Mutagenesis of Entero Agrregative E.Coli.

Trained on the working Priniple of FACS Instrument & quantified

the lymphocytes of normal Healthy Humans & Cancer Patients.

Trained on FACS & Under the Scientist Guidance quantified Normal

Healthy Cells & Apoptotic Cell by FACS (Becton & Dickenson).

From To Training Address

2001 2001 Project Trainee -1 Agharkar Research Institute,

Mar June Agharkar Road, Pune, India

http://www.aripune.org/

Project Cloning, Expression & purification of Alkaline Proteases from

Bacillus Sphericus.

Studied the effect of Triton X 100, Tween-20 & DMSO Impact on

the Alkaline proteases at different time periods ( 0-72hrs) &

Checked the Enzyme Activity by lowry Method & Protein Yield by

Bradford Methods.

Trained on performed partial Purification of the protein by

Centrifugation, Ammonium Sulphate Precipitations& Dialysis

Methods.

From To Training Address

2001 2001 Production Training Pasteur Institute of India,

Mar June Coonor, India.

http://www.pasteurinstitutei

ndia.com/

Training Observed the DTP & Rabies Vaccine manufacturing & Quality

Control of the Vaccines in Large Scale.

Educational Background:

From To Qualification % Name of the University

Secure

d

2000 2002 Master of Science in 70% Acharya Nagarjuna

June Mar Biotechnology University Campus, Guntur,

Andhra Pradesh, India,

Asia.

1997 2000 Bachelor of Science in 70% P.B. Siddhartha College of

June April Chemistry Arts & Science.

Vijayawada, Andhra

Pradesh, India, Asia.

From To

2002 Till Overall Technical Skills &

Mar Date Expertise

Overall Direct, Indirect and Sandwich ELISA for Quantification of

Technical Various Molecules & Bio-similar Molecules.

Skills & Cloning of PCR product into Clonejet PCR Cloning Kit (Fermentas)

Expertise & Cloning of genes into Expression Vectors.

Bioassay of Blood Clotting Factor by using Kabi Coatest Kit.

Transfection of target DNA Inserted into Mammalian Expression

Vector by Lipofectamine 2000 (Invitrogen) & Dreamfect Methods.

Isolation of lymphocytes from Human blood by using Ficoll -Paque

Plus.

Isolation of mRNA by Polytract kit (Promega).

RNA isolation by Trizol, RNAgent methods and RNA estimation by

Ribogreen Method.

cDNA Synthesis by first strand cDNA synthesis kits (Invitrogen &

Promega Kits).

Extraction of DNA from Agarose Gel by using Q1 AQuick Kit

method.

Isolation of plasmid DNA by using standard kits like Promega,

Eppendorf & iNTRON kits.

Genomic DNA Isolation from Mammalian cells by standard Methods

Separation of Receptor bound ligand from unbound ligand by Pall

Vacuum Chamber.

Clarification of the proteins by using Millipore Millistak

filters membranes.

Estimation of proteins & RNA by Bradford, BCA, Biuret, Ribogreen

& Folin's method.

Detection of Protein's by Native-PAGE, SDS-PAGE & Western

blotting Techniques.

Purification of recombinant proteins by using HPLC, AKTA

Purifier & AKTA Explorer systems.

Quantification of RNA, DNA & Proteins by using Nanodrop system

and Shimadzu-Spectrophotometer

Observation on large-scale production & Purifiaction of DTP

&Rabies Vaccines.

Lyophilization of recombinant expressed protein by using Lyolab

Lyophilizer system.

Bioinformatics Software's & Computer skills:

. Similarity search and sequence alignment: Clone Manager, NCBI BLAST,

EBI- Fasta, EBI-Clustal W. Excellent computing skills and proficient

in the use of Word, Excel, PowerPoint, Adobe Photoshop, Graphpad

Prism.Unique ability to learn and work on various computer

applications and software efficiently.

References:

Available upon Request

Contact this candidate