Project Engineering
Resume:
JIANPING SUN
Belmont, CA *****
*********@*****.***
A motivated and result-driven molecular biologist, microbiologist and
biochemist with wide range of knowledge in chemistry, data analysis, hands-
on experience in high throughput automation and a proven track-record of
successful delivery meet tight timelines.
EXPERIENCE
Sr. Scientist, metabolic engineering and systems biology
2012-2013
Scientist II, Fungal platform development
2011-2012
Intrexon, San Carlos, CA
. Designed and constructed vectors, engineered into strains with
improved (>150 fold) yield, titer, productivity and composition to
meet the project milestone ahead of time.
. Led a team (4 person) and developed a high-throughput
transformation platform for enzymes/proteins production
. Tech transferred and developed gene shuffling (mutagenesis)
protocol in house
. Engineered strains in both eukaryotic and prokaryotic hosts to
produce chemicals/enzymes
. Managed project, presented data in the group meeting and wrote
IDFs, technical reports and SOPs
. Assisted to develop commercial research proposal
Scientist (Collaboration)
2010-2011
Allopartis Biotechnologies, Inc.
. Expressed bacteria/fungal cellulases from AlloScreen in N. crassa
Associate Specialist
2011
Postdoctoral Associate
2008-2010
UC Berkeley, Energy Biosciences Institute
. Engineered strains with better cellulolytic enzyme production or
higher efficiency
. Characterized several key transcriptional regulators in fungi
involved in biomass degradation by transcriptome and secretome
analysis
. Developed N. crassa as a host for homologous and heterologous
recombinant expression of lignocellulolytic enzymes
Postdoctoral Associate, Microbiology and molecular cell biology
2006-2008
UC Berkeley, Plant and Microbial Biology
. Comprehensively evaluated protein-protein interactions during
heterokaryon incompatibility in N. crassa through LC-MS/MS, MASS,
yeast two hybrid and in vitro co-immunoprecipitation and
demonstrated the necessity for further research
. Characterized phcA from Pseudomonas syringae interacts with HET-C
alleles to induce programmed cell death in N. crassa
PhD candidate, Biochemistry and molecular biology
2000-2005
University of Science and Technology of China
. Participated in Human Structural Genome Project (HSGP) of China
. Established Structural Genome Center together with colleagues
. Characterized/determined the function and structure of Kti11p in
Saccharomyces cerevisiae
. Mentored undergraduate students as a teaching assistant
TECHNICAL EXPERTISE
. Molecular Biology, Microbiology and Metabolic Engineering
. Fungi, Saccharomyces cerevisiae, E. coli and other bacteria high-
throughput cultivation
. Fungal high-throughput transformation/transformants screening, and
bacterial high-throughput conjugation
. DNA/RNA extraction; vector design and high throughput construction;
gene knock-out/knock-in
. PCR, RT-PCR, RNA-seq, functional genomics, secretome and
transcriptome analysis
. Genetic manipulation and pathway engineering
. Electronic microscope/imaging
. Bioinformatics analysis; systems biology
. Synthetic biology and Biochemistry
. Enzyme evolution with random or structure based mutagenesis, and
gene shuffling
. Small scale (shake flask) fermentation and high-throughput strain
screening
. Familiar with automation equipment, such as liquid handing system
(BioMek), QExpression and Qpix
. Protein expression and characterization; Ni-NTA, HPLC, FPLC and
antibody based protein purification
. GC based small chemical quantification
. Co-immunoprecipitation, western blot, and ELISA
PATENT
International Patent Application No PCT/US2010/043279: Methods and
compositions for improving sugar transport, mixed sugar fermentation, and
production of biofuels
INVENTION DISCLOSURE
A method to convert nature gas into biofuels by methanotrophs
SELECTED PUBLICATIONS (out of 13)
1. Schmoll M, Tian C, Sun J, et al. Unravelling the molecular basis for
light modulated cellulase gene expression - the role of photoreceptors
in Neurospora crassa. BMC Genomics. 2012 Mar 31;13(1):127.
2. Sun J, et al. Deciphering transcriptional regulatory mechanisms
associated with hemicellulose degradation in Neurospora crassa. 2012
Feb; Eukaryotic cell
3. Sun J and Glass NL. Identification of the CRE-1 cellulolytic regulon in
Neurospora crassa. PLoS One. 2011; 6(9): e25654
4. Sun J, et al. Expression and characterization of the Neurospora crassa
endoglucanase GH5-1. Protein Expr Purif. 2011 Feb;75(2):147-54.
5. Tian C, Beeson W, Iavarone AT, Sun J, et al. Systems analysis of plant
cell wall degradation by the model filamentous fungus, Neurospora
crassa. Proc Natl Acad Sci USA. 2009 Dec 29; 106(52): 22157-62.
6. Wichmann G, Sun J, et al. A novel gene, phcA from Pseudomonas syringae
induces programmed cell death in the filamentous fungus Neurospora
crassa. Mol Microbiol. 2008 May;68(3):672-89.
7. Sun J, et al. Solution structure of Kti11p from Saccharomyces cerevisiae
reveals a novel zinc-binding module. Biochemistry. 2005 Jun
21;44(24):8801-9.
EDUCATION
Ph.D. Biochemistry and Molecular Biology
2005
School of Life Sciences, University of Science and Technology of
China (USTC)
B.S. Chemistry
2000
School of Chemistry and Chemical Engineering, Zhengzhou
University, China
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