Plant Software

FARHANA RUNA

**** ***** ***** **. *** # ***, Raleigh, NC 27606

Tel: 626-***-**** (c), *****@****.***

CURRENT POSITION

****- *******.

Ph.D. Student, Department of Plant Pathology, North Carolina State University (NCSU).

EDUCATION

M.S. in Plant Pathology & Microbiology (May 2007)

Division of Plant Sciences, the University of Arizona

M. Sc. in Microbiology: (Aug. 2001)

University of Dhaka

B.Sc. (Honors) in Microbiology (Oct. 1999)

University of Dhaka

APPOINTMENTS

July 2004-May 2007: Graduate student, Department of Plant Sciences, and Division of Plant

Pathology, the University of Arizona.

January 2003 July 2004: Rotation student, Department of Plant Sciences, and Division of

Plant Pathology, the University of Arizona.

February 2002 - July 2002: Research Associate, Department of Biochemistry and Molecular

Biology, University of Dhaka.

July 2001 - February 2002: Scientific Officer, Enteric Bacteriology Laboratory, Laboratory

Science Division, International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B).

November 1999 June 2001: Graduate (Master s) Student, Department of Microbiology,

University of Dhaka and Armed Forces Institute of Pathology (AFIP), Dhaka Cantonment.

PUBLICATIONS & PRESENTATIONS

Papers in Peer Reviewed Journals

1. Runa, F., Yasmin, M., Hoq, M. M., Begum J., Rahman, A. M. M. and Ahsan, C.R., (2011),

Molecular versus conventional methods: Clinical evaluation of different methods for

the diagnosis of tuberculosis in Bangladesh, Journal of Microbiology, Immunology and

Infection, Vol.44, Issue 2, pp. 101-105.

2. Runa, F., Park, M. S. and Pryor, B. M., (2009), Ulocladium systematics revisited:

phylogeny and taxonomic status, Journal of Mycological Progress, Vol. 8, Issue 1 pp. 35-

47.

Poster Presentations

1. Runa, F., Carbone, I., Bhatnagar, D. and Payne, G.A. (2011), Evidence of nuclear

heterogeneity in conidial populations of Aspergillus flavus . Poster presented at the Duke

Symposium in Celebration of Mycology and Mycologists, held in Duke University, North

Carolina on April 5-6, 2012.

2. Runa, F., Carbone, I., Bhatnagar, D. and Payne, G.A. (2011), Heterokaryons and diploids

in Aspergillus flavus derived from protoplast fusion . Poster presented at the 7th Annual

NC State University Graduate Research Symposium, held in McKimmon Center, North

Carolina on March 20, 2012.

3. Runa, F., Carbone, I., Bhatnagar, D. and Payne, G.A. (2011), Establishment of

heterokaryons in Aspergillus flavus by protoplast fusion and analysis of their stability

and migration in hyphae. Chalk talk presented at the 25th Annual Plant Molecular Biology

Retreat, held in Ashville, North Carolina from September 30 to October 2, 2011.

4. Runa, F., Carbone, I., Bhatnagar, D. and Payne, G.A. (2011), Multinucleate conidia of

Aspergillus flavus . Poster presented at the 3rd Annual PhD Symposium, Department of

Plant Pathology, and North Carolina State University on April 15, 2011.

5. Runa, F., Carbone, I., Bhatnagar, D. and Payne, G.A. (2011), Nuclear condition in

Aspergillus flavus during growth and conidiation. Poster presented at the Genetics of

Maize Disease Workshop, held in Renaissance Raleigh North Hills from February 20 to

February 23, 2011.

6. Runa, F., Hong, S.G., and Pryor, B.M., (2005), Developing an Agrobacterium tumifaciens

Mediated Transformation System for Alternaria arborescens Poster No. 549,

Presented at the Annual Meeting of American Phytopathological Society (APS), held in

Austin, Texas, USA from July 29, 2005 to August 3, 2005.

7. Pryor, B. M., Hong, S. G., and Runa, F., (2005), Multi-locus Analyses Reveal Polyphyletic

Origins of the Genus Embellisia Poster No. M-634, Presented at the International Union

of Microbiological Societies Meeting (IUMS) held in San Francisco, California, USA from

July 23, 2005 to July 28, 2005.

INVITED TALKS & ORAL PRESENTATIONS

1. Runa, F. and Pryor, B. M., (2006) Phylogenetic Relationships among Ulocladium and

Related Alternaria and Embellisia spp. Based upon rDNA and Protein Coding Genes

Presented at the Annual Meeting jointly organized by American Phytopathological Society

(APS), Canadian Phytopathological Society (CPS) and Mycological Society of America

(MSA), held in Quebec, Canada from July 28, 2006 to August 2, 2006.

2. Comprehensive Phylogenetic Revision of Alternaria and the Development of Efficient

Transformation Systems. (2005), Seminar presented in the Department of Plant Sciences,

(Division of Plant Pathology and Microbiology), The University of Arizona, USA.

3. Development of Agrobacterium tumifaciens mediated transformation method in

Alternaria arborescens (2004), Seminar presented in the Department of Plant Sciences,

(Division of Plant Pathology and Microbiology), The University of Arizona, and USA.

4. Inactivation of RsmA Removes the Need for GacS /GacA Two Component Regulation

(2003), Seminar presented in the Department of Plant Sciences, (Division of Plant Pathology

and Microbiology), The University of Arizona, USA.

5. Cloning and Mutagenesis of ybiB Gene of Azotobacter vinelandii (2003), Seminar

presented in the Department of Plant Sciences, (Division of Plant Pathology and

Microbiology), The University of Arizona, and USA.

DISSERTATION PUBLISHED

1. Development of an Agrobacterium tumefaciens-Mediated Transformation System in

Alternaria and Systematic Revision of Ulocladium Based upon Morphological and

Molecular Characterization of Species (2007), M.S. thesis submitted in the Department of

Plant Sciences, Division of Plant Pathology & Microbiology, the University of Arizona.

2. Evaluation of the Clinical Utility of Different Diagnosis Methods for Mycobacterium

tuberculosis . (2001), M.Sc. Thesis submitted in the Department of Microbiology, University of

Dhaka for the Degree of Master of Science (M.Sc.).

TEACHING EXPERIENCE:

Teaching Assistant (Fall 2011) - PP 730 Fungal Genetics and Physiology.

TECHNICAL EXPERTIES:

Molecular Genetics: DNA isolation, DNA cloning, DNA sequence analysis, preparation and

labeling of DNA probes, southern hybridization with non-radio labeled probes. Different

types of PCR (PCR-RFLP, PCR-RAPD, PCR-AFLP, RT-PCR). Bacterial transformation,

fungal transformation (random insertion mutagenesis by Agrobacterium tumefaciens,

protoplast mediated transformation and protoplast fusion).

Systematics: Phylogenetic analysis, raw sequence editing and polishing, alignment.

Software expertise: PAUP, McClade Phylogenetic software, MrBayes for Bayesian

Inference of Phylogeny, Model Test 3.7

DNA sequence Analysis software: Chromas, GCG Sequence Analysis Software Package,

BioEdit, FinchT and Sequencher.

Software expertise: SNAP Workbench for Population genetics data analysis

Microbiology/Mycology: Gram staining, Zeil-Neelsen staining, DAPI and PI staining;

antibiotic susceptibility testing (disk diffusion, E-test strips, broth and agar dilution, MIC

determination); MB/BacT rapid culture technique. Detection and sorting of fungal nuclei

expressing nuclear fluorescent proteins FACS (Fluorescence Activated Cell Sorter).

Software expertise: ANOVA STAT 32, FlowJo7.

Immunology and Serology: Bacterial cell surface protein and whole cell extract preparation;

SDS-PAGE, Commassie Brilliant Blue and silver staining; Western blot analysis; ELISA; gel

diffusion. Spectrophotometric enzyme assays; protein quantitation; serum separation from

blood; different serological and agglutination tests.

EXPERTISE WITH USE OF MICROSCOPE

Light, Dark, Phase contrast, Fluorescence and Confocal Microscope.

Software expertise: Imaging Software, ZEN2009, Image J, Photoshop.

PROFESSIONAL AFFILIATION & MEMBERSHIP:

Member of the Mycological Society of America (MSA).

Member of American Phytopathological Society (APS).

Member of Plant Pathology Graduate Student Association at NCSU.

Member of Joint Committee of Women in Plant Path & Cultural Diversity Committee.

Member of Graduate Microbiology Associations, North America (GMA-NA).

Member of Graduate Microbiology Associations (GMA) in Bangladesh.

SCHOLARSHIPS AND AWARDS:

Received Graduate Research Assistantship from the Department of Plant Pathology, North

Carolina State University.

Received National Science Foundation (NSF), USA, research grant for research during M.S.

Studies at the Department of Plant Sciences, The University of Arizona.

Received NST Fellowship (National Science and Technology Fellowship) for M.Sc Thesis,

Received Scholarship from the University Grants Commission on the basis of out standing

result in the B.Sc. (Honors)

Received scholarships in SSC and HSC based on the academic excellence.

Awarded from the Best Undergraduate Academic Achievement from the Department of

Microbiology, University of Dhaka for the outstanding academic performance in the B.Sc

(Hons).

Awarded Best Graduate Student for year 2000, by Araihazar Association.

Received certificate for actively participation and successful completion of a workshop on

HPLC (Practical and Theoretical) organized by Graduate Biochemists Associations, on

February 9, 2002.

Received certificate from the National Productivity Organization, Ministry of Industry,

Dhaka, for successfully completed the course Development of Productivity Technology

from February 10 to February 23, 2002.

REFERENCES:

1. Dr. Garry A. Payne

Professor

Department of Plant Pathology

North Carolina State University

Email: **********@****.***

Phone: 919-***-****

2. Dr. Ignazio Carbone

Associate Professor

Department of Plant Pathology

Email: ***************@****.***

Phone: 919-513- 4866

3. Dr. Barry M. Pryor

Associate Professor

Department of Plant Sciences

Division of Plant Pathology & Microbiology

The University of Arizona

Email: *******@*.*******.***

Phone: 520-***-****

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