Sales Data

Christina Nolan

Email: *********@********.***

Address: ***** ****** **

City: Germantown

State: MD

Zip: 20874

Country: USA

Phone: 919-***-****

Skill Level: Experienced

Salary Range: 49

Willing to Relocate

Primary Skills/Experience:

See Resume

Educational Background:

See Resume

Job History / Details:

CHRISTINA K. NOLAN

13523 Duhart Rd, Germantown, MD 20874

919-***-**** *********@********.***

TECHNICAL SKILLS

High Performance Liquid Chromatography (HPLC)

Mass Spectrometry (LC/MS/MS)

Liquid Scintillation Spectroscopy (LSS)

Robotic liquid handling systems

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Immunofluorescence staining

BCA protein assay

UV/vis plate reader

RNA purification & quantification

cDNA cloning and RT-PCR

Western blotting

Light microscopy

EXPERIENCE

Xceleron (via TechUSA), Germantown, MD

Analyst (Mar 2012-Jul 2012)

* Receive, process and analyzed human biological samples from Sponsors or Contract Research Organizations using defined analytical methods.

* Generated results of study/method development work and making recommendations for ongoing work.

* Developed analytical methods for sample preparation, HPLC fractionation and AMS (accelerated mass spec) analysis of samples.

* Carried out data processing, QC checking, and data compilation in preparation for report writing.

Life Technologies, Durham, NC (formerly CellzDirect/Invitrogen)

Scientist II, Production, Primary and Stem Cell Systems Division (Feb 2008-Mar 2012)

* Performed cell culture assays on cryopreserved primary mammalian cells and cell lines, assessing functional activity and cellular morphology characteristics to determine optimal application for customer use, including metabolic and transporter assays in suspension and plated cells: induction, specific activity (CYP450, FMO and phase II enzymes), intrinsic clearance/metabolic stability, and transporter uptake.

* Extracted biological samples and analyzed via HPLC and LC/MS/MS analysis in accordance with GLP regulations.

* Integrally involved in tech transfer on analytical capabilities to Durham, NC facility in 2010-2011: trained on LC/MS/MS and HPLC protocols, completed data processing and QC reviews of performance qualifications and sample analysis, responsible for setting up liquid handler (Matrix Hydra DT) for sample extractions.

* Developed and validated numerous assays in 2008-2009, increasing the depth of our product catalogs which contributed to a 100% increase in human cryopreserved product sales in 2009.

* Completed sixteen R&D projects in 2008 alone, working both independently and collaboratively with team members, demonstrating technical capabilities and attention to detail and contributing to posters and publications.

* Processed and reviewed data for LC/MS/MS method development.

* Extracted and analyzed radiolabeled samples via Liquid Scintillation Spectroscopy (LSS) to characterize OATP and OCT cellular transporter mediated uptake functions.

* Collaborated with QA to improve documentation which significantly decreased labor hours required for batch release.

* Reduced cycle time for batch release by two-thirds in 2010, via streamlined processes and improved scheduling with our remote office. Enabled predictive release of product batches while satisfying requirements of sales, operations and QA teams.

* Routinely shipped flammable and biohazardous materials domestically.

Environmental Conservation Labs, Cary, NC

Wet Chemistry Analyst (July 2007-Feb 2008)

* Performed sample preparations (organic extractions) on soil and water samples, preparing them for GC and GC/MS analysis in order for identification of organics in environmental field.

* Initiated in house pipette calibration program.

* Performed wet chemistry techniques on drinking water, municipal and industrial wastewater, soil, groundwater, and hazardous waste samples according to EPA methods including titrimetric, gravimetric, nephelometric, colormetric, and ion-selective electrode analysis for environmental site assessments and monitoring programs in compliance with EPA guidelines and regulations.

RTI International, Durham, NC

Chemist II, Drug Metabolism and Pharmacokinetics (DMPK) Department (March 2005-June 2007)

Chemist I (promoted May 2006)

* Operated HPLC and LSS to generate and analyze data in support of GLP regulated studies.

* Co-executed GLP regulated studies using electronic data capture (Lablogic Debra 5).

* In support of GLP regulated pharmokinetic studies, utilized medical techniques, including blood collection from rodents via cardiac puncture, tail vein, and cannula; euthanasia of rodents; clinical sign observations, necropsy, and tissue collection of rats; cutaneous (dermal) application of test article to rats; and compound administration by feed to rodents.

* Managed chemical inventory for individuals within DMPK and ordered custom inventory as needed.

* Performed in vitro assays of CYP450 activity with potential inhibitors using microsomal tissue sources and metabolic stability assays using S-9 fractional homogenates.

* Developed automated assays utilizing robotics programming for high throughput metabolic screening.

EDUCATION

Biochemistry, B.S., Magna Cum Laude, North Carolina State University, Raleigh, NC, Dec 2003

Chemistry, B.S., Magna Cum Laude, North Carolina State University, Raleigh, NC, Dec 2003

SOFTWARE

Microsoft Office Suite

Oracle EnterpriseOne

Waters MassLynx

Waters Empower

Lablogic Debra

Shimadzu LCsolution

Graphpad Prism

Collect XL

Waters Millenium

Agilent Chemstation/Chemstore

HONORS

Life Technologies On the Spot Award for exceptional performance 2009, 2010, 2011, 2012

RTI Spot Award for exceptional performance on a study 2005, 2007

PUBLICATIONS & POSTERS

C.M. Smith, C.K. Nolan, M. Edwards, J. Hatfield, T.W. Stewart, S.S. Ferguson, E.L. LeCluyse, and J. Sahi, -A Systematic Evaluation of the Metabolic Capacity of Suspension and Conventionally Cultured Cryopreserved Human Hepatocytes,- Journal of Pharmaceutical Sciences 101 (2012)

C. Smith, C. Nolan, M. Edwards, T. Stewart, J. Hatfield, J. Hill, J. Sahi and E. LeCluyse, -Comparison of Metabolic Capacities of Fresh and Cryopreserved Human Hepatocytes Isolated from the Same Donor: Metabolic Stability, Plated Metabolism, Metabolite ID Applications.- Drug Metabolism Reviews, (2009) 40 (2) 223.

S. Otmani, C. Smith, A. Gupta, P. Altiero, M. Ribadineira, C. Nolan, S. Barfield and E. LeCluyse, -Determination of Intrinsic Clearance Values for Metabolically Stable Compounds Utilizing Plateable Cryopreserved Human Hepatocytes.- Drug Metabolism Reviews, (2008) 40 (3) 243.

ADDITIONAL EDUCATION & TRAINING

Good Laboratory Practices (GLP) Training, RTI (2005-2007), Life Technologies (2008-2011)

Laboratory Safety, Life Technologies (2008-2011)

Bloodborne Pathogens, RTI (2005-2007), Life Technologies (2008-2011)

Radiation safety, RTI (2005-2007), Life Technologies (2008-2011)

Shipping of hazardous materials, Life Technologies (2009-2011)

Canine and rodent husbandry for GLP studies, RTI (2006-2007)

Blood collection of rats and mice via cardiac puncture, tail vein and cannula for GLP studies, RTI (2005-2007)

Oral gavage of rats for GLP studies, RTI (2005)

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