Quality Assurance Control

PRASAD GAJARE

***, ***** **** **., ***. D*,Westknoll apartments, Newark, DE-19711, USA.

Phone: 724-***-**** Email- *******@****.***, *********@*****.***

OBJECTIVE

Looking for a challenging position as a Bioinformatics intern, where in my technical, computational and

software skills can be utilized. I intend to contribute positively to the position, considering my Pharmacy

background, excellent communication skills and a systematic approach to details.

EDUCATION

Masters of Science, Bioinformatics January 2014

University of Delaware, Newark, DE

Cumulative GPA : 3.667

Relevant Coursework

Bioinformatics programming for biologists, Biostatistics, Writing Academic research

Database Systems, Bioinformatics,

Independent study (Next generation sequencing, Reduced representation,SNP), Data Mining.

System Biology, Ethical Issues in Global Business Environment.

Bachelors of Pharmaceutical Sciences June 2010

MET Institute of Pharmacy, University Of Mumbai

Cumulative GPA: - 3.11

COMPUTER SKILLS

Programming skills : Perl, Python, Shell scripting, Bash, R, C,C++, Java.

Software Skills : Weka, JMP, BWA, SAMTools. USEARH, CH,CD-HIT ClustalW.

Operating Systems : Linux, Windows, Mac OS.

Miscellaneous : Libre Office, Microsoft Office, PIR, NCBI, DAVID, iHop, eGift, UniProt,

BLAST, Fasta, FASTQ.

Database: MySQL

PROJECTS

Protein annotation project

Annotated little skate Cytochrome b protein using RACE-P server.

• Identified of a candidate protein from UniProtKB. Sequence analysis for candidate protein

(pro tein similarity search and protein domain analysis).

• Completed literature review for candidate protein (Used PubMed as a resource, and did the review

based on functional annotation and experimental characterization)

• Identified of the candidate protein on genomic / transcriptomic contigs using skate blast

• Annotated Protein using RACE-P

• Tools used – Philius, Phobius, TMHMM server, Signalp, Emboss-tmap, Netphos

Reduced representation and next generation sequencing

Literature review of PacBio Lifesciences, Reduced representation

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Used reduced representation techniques in Lima Beans project.

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Written scripts using Perl, for analysis of SAM file, scripts for parsing Fastq file (on average

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quality score and sequence length)

Used BWA and SAMTools to find various traits of Lima beans FASTA file database.

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A database of conference papers

A complete database of conference papers from various journal sources, was developed. The

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RDBMS used in the application is MySQL and it is deployed on a Linux OS. The back end cod ing

is done using Java. The UI is developed as a command line application. The administrator,

Conference papers library user and author are the three functionalities designed in the application.

The authorized administrator can manage the users and authors list and also all modify/delete/add

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the conference papers.

The authorized user can view the papers, based on a specific search criteria.

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The authorized author can add papers to the database, and modify / delete his own papers.

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Literature review of next generation sequencing

Completed a literature survey and wrote a review paper on the various methods of next generation

sequencing.

EXPERIENCE

¬ Intern, AUROCHEM Pvt. Ltd., Thane, India (June, 2009)

Responsibilities:

Worked in the Quality Control, Quality Assurance and Analytical development

departments.

Operated the HPLC system, GC system and Dissolution apparatus.

Assisted in the production of tablets, capsules, ointments, emulsions, suspensions and

parentals.

¬ Pharmacist, Shree Hasu Medical Stores, Mumbai, India (June – December 2010)

Worked as a Pharmacist

¬ Pharmacy Incharge, Jugura Hospital, Mumbai, India (January – July 2011)

Worked in hospital as a Pharmacy In charge

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