Manager Medical
Resume:
Faith A. Kaplan
************@*****.***
Winchester, MA 01890
SUMMARY: 5 years experience as manager and sole operator of a MoFlo (Dako) cell
sorter for a multi-user cell sorting facility as well as 15 years of general flow cytometry
instrumentation (4 years of FACS Canto with Diva software) expertise. Scientific
manager with extensive project and team leadership experience. More than 15 years of
hands on industrial laboratory experience in methods development for cell based assays.
Recognized for successfully contributing to the launching of a USA IND to the FDA
concerning in vivo large animal transplantation device study.
TECHNICAL:
*Currently, was executing daily functions as the lab manager in a new start up laboratory
at Boston University; included the overseeing of smooth daily operations of the laboratory
as well as the establishment of a new tissue culture facility, the purchasing of new lab
equipment for multiple “big ticket items” such as centrifuges, microscopes, digital
cameras, PCR machines, biological storage cabinets (BSC), as well as anything to do with
the flow cytometer.
*Executed daily ELISA’s and immunocytochemical staining of cell lines.
*Worked in a clinical research lab performing multiple flow cytometric profiling including
stem cell work ups on patients undergoing stem cell transplants and T cell subsets markers
for HIV and transplant patients.
*Sole operator of a MoFlo (DakoCytomation cell sorter) three laser system using a
MAPPIT system for the identification of oncology targets (small molecule) and drug
compound studies.
*Cloned cells for the Molecular Technology Group using a cell sorter (MoFlo).
*Oversaw all flow cytometric work including experimental design, sample acquisition, and
analysis of DNA (cell cycle), BrdU, phospho-cell profiling, and apoptotic assays.
*Implemented cell sorting using a FACS Vantage-SE for the isolation, purification, and
expansion of pancreatic stem/progenitor duct cells for the treatment of Type I diabetes.
*Indentified novel surface reagents pertaining to the characterization of progenitor
pancreatic duct cells to be utilized for cell sorting.
*Resolved immunological, biological and safety challenges regarding allogeneic versus
xenogeneic peripheral and central nervous system cellular implantation.
* Accomplished GLP level Safety and Toxicology trials using non-human primates
transplanted with encapsulated cell devices into the striatum and ventricle.
*Developed innovative in vitro and in vivo immune testing of organ-equivalents using
extensive cell culture expertise in human, canine, ovine, and rat systems.
Faith A. Kaplan page 2
(Technical; continued)
*Designed experimental models to evaluate donor-host interaction in a complex extra-
cellular matrix environment.
*Correlated and executed MHC studies selecting living-related renal and bone marrow
transplant recipients, and screening of cadaveric candidates for both liver and renal
transplantation by HLA-ABC and DR typing, MLRs, HLA antibody screening and
crossmatching.
*Performed all routine Blood Banking duties; included all donor room and blood component
duties as well as typing and crosssmatching patient samples.
MANAGEMENT:
*Executed daily functions as the lab manager to ensure the smooth daily operations in a
start-up laboratory.
*Responsibilities included the purchasing of all laboratory equipment in a new start up lab.
*Scheduled and taught personnel methods to be used on the flow cytometer.
*Generated SOP documents for the transfer of completed R+D projects to manufacturing.
*Instructed technical personnel in newly developed cell based assay protocols.
*Completed a written summation of three years of in vivo large animal model (sheep)
transplantation immunological data that was essential for a successful USA IND
submission to the FDA.
EXPERIENCE:
Boston University Medical Campus Boston, MA 5/2008 -12/2008
Lab Manager/Research Tech
Mass General Hospital Immunopathology; Diagnostic Flow Cytometry Lab
Med Tech II Boston, MA Nov 2007-Feb 2008
GPC-Biotech Waltham, MA 2000-2007
Manager Flow Cytometry (2005-2007)
Scientist (2000-2005)
Becton Dickinson Technology; Research Triangle Park, NC 1999-2000
Scientist
Cytotherapeutics Inc. Lincoln, RI 1990-1999
Associate Scientist/Associate Manager Immunology
Faith A. Kaplan Page 3
(Work Experience continued)
Organogenesis Cambridge, MA 1987-1990
Sr. Research Associate
New England Deaconess Hospital
Blood Bank/Tissue Typing Laboratory Boston, MA 1979-1987
MSMT (ASCP) SBB
Education
MS (Medical Laboratory Science); Northeastern University; Boston, MA
Specialist in Blood Banking SBB; New England Deaconess Hospital; Boston, MA
BS Medical Technology MT (ASCP); The Ohio State University; Columbus, OH
Personal
Member of ISAC 2005 – present
Boston (MoS) Museum of Science; VOLUNTEER 2001 – present
Active “hands-on floor interpreter” for both children and adults
in the Human Body Connection Division
CURRENT PAPERS:
M. Caliguiri, L. Molz. Q. Liu, F. Kaplan, J. Xu, J. Majeti, R. Kelsy, K. Murthi, A. Kluge, S.
Livens, J. Taverni, et N. Kley, “MASPITT: Three Hybrid Trap for Quantitative
Proteome Fingerprinting Interactions in Mammalian Cells.” Chemistry & Biology Vol 13
issue 7 July 2006, pp 711-722
M. Caliguiri, F. Becker, K. Murthi., F. Kaplan et al. “A Proteome-Wide CDK/CRK-Specific
Kinase Inhibitor Promotes Tumor Cell Death In the Absence of Cell Cycle Progression.”
Chemistry and Biology Vol. 12, 1103-1115 Oct. 2005
K. Wosikowski, L. Lamphere. F Kaplan, J Xu, B Rattel, M. Caliguiri, “Preclinical Anti tumor
Activity of the Oral Platinum Analog Satraplatin.” Manuscript # CCP-06-0333 Caner
Chemotherapy and Pharmacology in press
Faith A. Kaplan Page 4
(Current Papers; Continued)
Stabila P, Wong S, Kaplan F, and Tao W. "Cell surface expression of a novel human IgG
Fc chimera, in the reverse orientation, activates macrophages through Fc receptors."
Nature Biotechnology, Vol. 16 No 13 1357 - 1360- Dec 1998
Kaplan FA, Krueger PM, Harvey J, and Goddard MB. "Peripheral xenogeneic immunological
response to encapsulated bovine adrenal chromaffin cells implanted within the sheep
lumbar intrathecal space." Transplantation Vol 61 No.8, pp1215-1221, April 27, 1996
Aebischer P, Pochon NA, Heyd B, Deglon N, Joseph JM, Zurn AD, Baetge EE, Hammang JP,
Goddard M, Lysaght M, Kaplan F, Kato AC, Schluep M, Hirt L, Regli F, Porchet F, and
Tribolet N. "Clinical Protocol, Gene Therapy for Amyotrophic Lateral Sclerosis (ALS)
Using a Polymer Encapsulated Xenogeneic Cell Line Engineered to Secret hCNTF."
Human Gene Therapy, 7:851-860 May 1, 1996
Lavoie MP, Palmatier M, Gentile FT, Kaplan FA, Fiore DM, Hazlett TF, Bell WJ, and
Flanagan TR. "Two PC12 Pheochromocytoma lines sealed in hollow fiber-based capsules
tonically release L-dopa in vitro." Cell Transplantation, Vol 2, pp163-173, 1993
Theobald VA, Lauer JD, Kaplan FA, Baker KB, and Rosenberg M. "Neutral allografts-Lack
of allogeneic stimulation by cultured human cells expressing MHC class I and class II
antigens." Transplantation (55):128-133 No 1, January 1993
CURRENT ABSTRACTS:
Wosikowski, K., Caligiuri, M., Kaplan, F., Xu J., Kley, N., and Rattel. B., Efficacy of
Satraplatin (J216) and its Metabolite (JM118) is maintained in Tumor cells Resistant
to Cisplatin, Mitoxantrone and Campthocethin. Journal of Chemotherapy vol 16,
supplement 1 page 151 Feb. 2006
Cailigiuri, M., Bockovich, N., Oalman C., Kaplan F., et al. Induction of Tumor Regression by
the Broad-Spectrum Cyclin Dependent Kinase Inhibitor RGB-298638. 2006 AACR
meeting Washington, DC
Caligiuri, M., Bockovich N., Oalman C., Kaplan F. et. al. GPC-286199: A Potent Broad
Spectrum Inhibitor of the Cyclin Dependent Kinase. 2004 AACR meeting Orlando,
Florida
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