Vice President Pharmaceutical

SREE ANUMOLU

** ****** **, **********, **-*****, 732-***-**** (M), **********@*****.***

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EDUCATION

PhD candidate in Pharmaceutics Anticipated to graduate in August 2009

Rutgers-The State University of New Jersey, NJ

Advisor: Dr. Patrick J Sinko.

Department of Pharmaceutical Sciences, August 2003- July 2004

University of Missouri, Kansas City (UMKC), MO.

Advisor: Dr. Ashim K Mitra.

B.S in Pharmaceutical Sciences June 1999-May 2003

Nagarjuna University, India.

PHARMACEUTICAL INDUSTRY EXPERIENCE

Johnson & Johnson PRD, Raritan, NJ June 2005-August 2005

Summer Intern, Ortho Clinical Division (OCD)

Designed an HPLC assay to measure the concentration of GSH and GSSG in rat

hepatocytes.

Worked towards establishing stable transfected cell lines of OATP2 transporter in wild

type MDCK II cells.

Evaluated the transport of substrates of OATP2, 17-Estradiol β Glucuronide and Estrone

Sulfate in rat hepatocytes.

Fytokem Formulations Ltd, India May 2003-July 2003

Summer Intern, R&D

Evaluated the effect of a mixture of Eudragit L & S polymers on the in vitro dissolution

profiles of enteric coated tablets.

GRADUATE SCHOOL RESEARCH EXPERIENCE

Preformulation and Formulation

Conducted preformulation studies to evaluate the effect of drug solubility and

lipophilicity on drug loading efficiency in hydrogels.

Designed and developed in situ forming poly(ethylene glycol) hydrogels for topical

application to eyes and skin.

Evaluated the effect of formulation additives on the dehydration and retention time of

hydrogels on mice skin.

Investigated the stability and accelerated stability of drugs in hydrogels.

Evaluated the effect of crosslinking ratio, polymer and crosslinker concentrations on drug

loading and mechanism of drug release from different hydrogel formulations.

Evaluated the effect of the ratio of PLGA and PLLA on the drug loading and release

profiles of microspheres.

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Measured the drug concentration released in vitro using a Reverse Phase High

Performance Liquid Chromatography (RP-HPLC).

Evaluated the effect of formulation additives, crosslinking ratio, type of polymer and

crosslinker used and their concentrations on the crystallization temperature of hydrogels

using a Differential Scanning Calorimeter (DSC).

Evaluated the optical transmissivities of the hydrogels using a UV-Vis

spectrophotometer.

Evaluated the mechanical strength of hydrogels using a Rheometer.

In vitro

Evaluated the effect of solubility, lipophilicity and molecular weight of drugs on

permeation through wounded mice skin.

Investigated the permeation profiles of doxycycline through human cadaver skin,

wounded and unwounded mice skin and rabbits cornea.

Quantitatively measured different inflammatory mediators as endpoints in wound healing

of injured mice skin by RT-PCR and Western blot techniques.

In vivo

Prepared pharmacokinetic and pharmacodynamic study protocols, conducted studies, and

interpreted the data using WinNonlin.

Developed a wound model in mice to study the wound healing efficacy of topically

applied hydrogel formulations.

Conducted pharmacokinetic studies to investigate the bioavailability of topically applied

doxycycline in wounded and unwounded mice.

Conducted pharmacodynamic studies in mice to evaluate the wound healing response

after application of a topical spray on hydrogel dressing by measuring edema,

inflammatory biomarkers and histopathology.

Evaluated the histopathological effect of dose and time of exposure of nitrogen mustard

on the back of mice skin.

Evaluated the pharmacodynamic response of a hydrogel drug delivery system in rabbit

eyes by measuring miosis and intraocular pressure.

Collaborated with the Pharmacology and Toxicology department to evaluate the wound

healing efficacy of a doxycycline hydrogel delivery system in the excised rabbit’s cornea

using corneal organ culture model.

Proficient in handling rodent animal species including mice and rabbits.

Cell and Tissue culture

Performed MTT assay in Human Epidermal Keratinocytes to evaluate the safety of

different developed hydrogel formulations.

Evaluated the concentration and time dependent binding and uptake of RGD peptide in

Human Epidermal Keratinocytes.

Developed and optimized a transfection method for VEGF165 siRNA in HEK 293 cell

line.

Screened the retinal cell line, ARPE-19 to investigate the functional expression of

riboflavin transporters.

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TECHNICAL SKILLS

Analytical: HPLC, DSC, Rheometer, Dissolution apparatus, Liquid Scintillation

Counter, Fluorescent and UV-Visible spectrophotometers.

Bioanalytical: RT-PCR, Western blot, Immunofluoroscence assay, ELISA, MTT assay.

In vivo (Animals): Pharmacokinetic/Pharmacodynamic (PK/PD) studies in mice, PD

studies in rabbits.

Cell and Tissue culture: Uptake studies, Binding studies, Transport studies,

Transfection of cells.

Statistical data analysis: GraphPad Prism, WinNonlin, Kinetica, Microsoft Excel.

Scientific writing skills: Grant proposals, Manuscripts, Patents.

PATENT APPLICATIONS

P. J. Sinko, S. Stein, A. Menjoge, S. Gunaseelan and S. S. Anumolu, “Spray-on wound

dressing (dressing compositions)” United States Patent pending, Rutgers docket number

07-068 (filed March 28, 2008).

PUBLICATIONS

S. S. Anumolu, Y. Singh, D. Gao, S. Stein and P. J. Sinko, “Design and evaluation of

novel fast forming pilocarpine-loaded ocular hydrogels for sustained pharmacological

response”, Journal of Controlled Release (2009), 137(2), 152-9.

S. S. Anumolu, A. S. DeSantis, A. R. Menjoge, R. A. Hahn, J. A. Beloni, M. K. Gordon

and P. J. Sinko, “Doxycycline loaded poly(ethylene glycol) hydrogels for healing

vesicant-induced ocular wounds”, submitted.

S. S. Anumolu, A. R. Menjoge, R. S. Navath, S. Stein and P. J. Sinko, “Doxycycline

loaded poly(ethylene glycol) hydrogels with reversible crosslinks for topical wound

healing application”, submitted.

S. S. Anumolu, Y. C. Chang, D. Gao, A. R. Menjoge, S. Stein, Gerecke D. R and P. J.

Sinko, “Pharmacokinetic and pharmacodynamic evaluation of doxycycline hydrogels for

wound healing of vesicant-exposed mice skin”, in preparation.

S. S. Anumolu, D. Gao and P. J. Sinko, “Nitrogen mustard skin wound progression and

barrier property evaluation in a SKH-1 mouse model”, in preparation.

AWARDS

Travel Award from CounterACT (NIH/NINDS), April 2008.

Travel award from Pharmaceutics and Drug Delivery (PDD) section of American

Association of Pharmaceutical Scientists (AAPS), November 2007.

Graduate Student Travel Award from Rutgers University to attend AAPS, November

2007.

Best Poster award, Graduate Research Association of Students in Pharmaceutics

(GRASP) symposium, June 2007.

Graduate Student Travel Award from Rutgers University to attend AAPS, October

2006.

K.V.S. R Memorial gold medal for academic achievement, February 2004.

Alapati Jaya Sree Memorial gold medal for being the top student of Nagarjuna

University, January 2004.

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Indian Pharmaceutical Association (IPA) Dharmadatha gold medal for academic

excellence, November 2003.

Best Poster award, Pharmaceutical National Conference, India, March 2003.

Best Essay award, Indian Pharmaceutical Congress (IPC), December 2001.

PROFESSIONAL ACTIVITIES AND AFFILIATIONS

Treasurer, Graduate Research Association of Students in Pharmaceutics (GRASP)

committee, 2006-2007.

President, Graduate Student Association of Pharmaceutics, 2006-2007.

Vice President, Graduate Student Association of Pharmaceutics, 2005-2006.

Member, American Association of Pharmaceutical Scientists (AAPS), 2005-2009.

Member, Graduate Student Association of Pharmaceutics, 2004-2009.

PRESENTATIONS

A. Menjoge, S. S. Anumolu, S. Stein and P. J. Sinko, “In-situ gelling bioadhesive

polyethylene glycol networks with reversible crosslinks as hydrogels for drug delivery “;

poster presented, CounterACT Network Research symposium, April 2009.

A. Menjoge, S. S. Anumolu, S. Stein and P. J. Sinko, “In-situ gelling bioadhesive

polyethylene glycol networks with reversible crosslinks as hydrogels for drug delivery “;

poster to be presented, AAPS symposium, November 2008.

S. S. Anumolu, R. Navath. S. Stein and P. J. Sinko, “Formulation of a novel in situ

forming hydrogel for sustained topical delivery of doxycycline hyclate for treatment of

sulfur mustard injuries”; poster presented, CounterACT Network Research symposium,

April 2008.

S. S. Anumolu, R. Navath. S. Stein and P. J. Sinko, “Formulation of a novel in situ

forming hydrogel for sustained topical delivery of doxycycline hyclate for treatment of

sulfur mustard injuries”; poster presented, AAPS symposium, November 2007.

R.Navath, S. S. Anumolu, S. Stein and P. J. Sinko, “Formulation of a novel spray

hydrogel for topical drug delivery”; poster presented, AAPS symposium, November

2007.

S. S. Anumolu, Y.Singh. S. Stein and P. J. Sinko, “Characterization of a novel in situ

forming hydrogel for sustained ocular drug delivery”; poster presented, GRASP

symposium, June 2007.

S. S. Anumolu, Y.Singh. S. Stein and P. J. Sinko, “Characterization of a novel in situ

forming hydrogel for sustained ocular drug delivery”; poster presented, AAPS

symposium, October 2006.

S. S. Anumolu, “Human gene therapy, treatment of the future?”; poster presented,

Pharmaceutical National Conference, India, March 2003.

S. S. Anumolu. “Future of pharmacists in India”; essay published in the 53rd IPC, T16-

T20, December 2001.

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