Assistant University

Isha Wallace

**** ***** ****

Colton, CA *****

Cell: 909-***-****

Home: 909-***-****

Lab: 951-***-****

****.*******@*****.***

EDUCATION

University of California, Riverside Riverside, California

2003 to Present

Ph.D. in Cell, Molecular and Developmental Biology

Anticipated Defense Date: March 2010

University of Redlands Redlands, California

1999-2003

B.S., Biology

PROFESSIONAL EXPERIENCE

University of California, Riverside Riverside, California

Spring, Fall 2009

Teaching Assistant - Cell Biology

Winter, Spring 2008

. Prepared and presented discussion lectures on cell biology and molecular

techniques.

. Composed quizzes and exam questions.

. Graded quizzes, exams, and reports.

University of California, Riverside Riverside, California

Summer 2009

Teaching Assistant - Human Heredity and Evolution

. Led discussions on human genetics and microevolution.

. Provided students with assistance on scientific writing.

. Graded quizzes, reports, oral presentations, exams and homework problems.

University of California, Riverside Riverside, California

Fall 2006, 2008

Teaching Assistant - Introduction to Biology Laboratory

. Prepared and presented lectures on theoretical background and lab

techniques.

. Provided students with assistance in scientific writing.

. Graded quizzes, exams, and lab reports.

University of Redlands Redlands, California

Spring 2001 - Spring 2003 Peer Tutor in Biology

. Taught lecture and laboratory material to individuals or groups of

students in introductory biology, genetics and molecular genetics.

. Edited/provided feedback on term papers and lab reports.

ACADEMIC EXPERIENCE

University of California, Riverside Riverside, California

2003 to Present

Graduate Student Researcher

. Conducted directed research for Ph.D. degree thesis under the direction

of Dr. Jeff Bachant.

. Experience using Saccharomyces cerevisiae as a model organism.

. Expertise in designing and constructing S. cerevisiae strains through

transformation of plasmids, gene knock-outs, and tetrad analysis.

. Expertise in protein biochemistry (protein purification, in vitro binding

and activity assays, immunoprecipitations).

. Expertise in oligonucleotide design and PCR mutagenesis techniques.

. Experience in fluorescence microscopy and immunohistochemisty.

. Experience in subcloning, designing plasmids and plasmid purification

from E. coli and yeast.

. Experience with chromatin immunoprecipitation in yeast.

. Expertise in yeast centromeres and kinetochores.

. Experience with assay development, optimization and trouble-shooting.

University of Redlands Redlands, California

Summer 2001, 2002-2003

Research Assistant - Dr. Linda Silveira's Lab

. Mutagenized and screened K. lactis budding yeast strains for cell

division cycle (cdc) phenotypes.

Cloned and characterized cdc mutants in K. lactis using light microscopy.

Verified isolation of a cdc14 mutant strain using subcloning techniques.

Designed PCR primers for amplification of K. lactis CDC14 used in

sequencing reactions and submitted the gene sequence to GenBank.

University of California, Riverside Riverside, California

Summer 2002

Research Experiences for Undergraduates (REU) Program - Dr. Natasha

Raikhel's Lab

Isolated RNA and used RT-PCR to determine the mRNA expression patterns of

two xylosyltransferases in Arabidopsis.

. Screened for homozygous T-DNA mutagenized gt7 plants using PCR.

. Conducted database searches for GT7 orthologs.

ACADEMIC & PROFESSIONAL HONORS

NSF Minority Grant Supplement

2004

University of Redlands B.S. in Biology with Honors

2003

Research Experiences for Undergraduates (REU) Program

2002

University of Redlands Dean's List

1999-2003

Outstanding Peer Tutor of the Year Award

2003

University of Redlands Achievement Award

1999-2003

University of Redlands Presidential Scholarship

1999-2003

TECHNICAL SKILLS

Broad knowledge of cell biology, molecular biology and genetics

Worked in a multidisciplinary laboratory environment. Research integrated

molecular biology techniques, genetic screens and manipulations, and

biochemistry.

Molecular Biology

DNA and RNA isolation and purification, DNA sequencing, PCR, RT-PCR,

electrophoresis (nucleic acid and protein), subcloning, site-directed

mutagenesis.

Cell Biology

Transformations, cell culture, aseptic techniques, DIC microscopy,

fluorescence microscopy, confocal microscopy.

Protein

Protein isolation and purification, SDS-PAGE, western blotting,

immunoprecipitation, ChIP, in vitro kinase assays.

Computer

Proficient in Microsoft Office, Adobe Photoshop, DNA strider (primer

design, plasmid construction), basic bioinformatics software (i.e. BLAST)

and image quantitation software (e.g. NIH ImageJ). Familiar with C/C++

programming.

PROFESSIONAL SKILLS

Operations

Trained lab members on proper lab procedures specifically related to

scientific protocols and equipment.

Developed and optimized laboratory protocols.

Organization of laboratory responsibilities and ordering of supplies.

Leadership/ Teamwork

CMDB Student Recruitment Committee member: planned Recruitment Day

activities and recruited students from undergraduate universities.

CMDB Entertainment Committee member: planned and coordinated special

events.

PRESENTATIONS

Wallace I, Kavanaugh S, Bachant J. The Kinetochore Protein Mif2 is

Targeted by Cdk1. (CSHL Yeast Cell Biology Meeting, August 2007)

Silveira LA, Oldroyd A, Munson A, Wallace I, Norris S, Reynolds S, Weller

S, Wilkerson S, Perez Van Worth C, So J, Sage A, Rawson J, Zoller K,

Paulhus R, Iannantuano D, Campos P. Cell Cycle Mutants of the Budding

Yeast Kluyveromyces lactis. (42nd American Society for Cell Biology Annual

Meeting, December 2002)

PUBLICATIONS

Wallace I, Bachant J. The Kinetochore Protein Mif2 is Targeted by Cdk1.

(In preparation)

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