Assistant Quality Control

Dinesh V. Palanivelu PhD ****, Granada Ave. Apt. *7

Santa Clara

Curriculum Vitae CA 95051 U.S.A.

Phone : 001 415-***-****

Fax : 001 415-***-****

E-mail : ******.**********@****.***

Career Objective

To actively participate in the drug discovery team through the process

of protein production and crystallization for structure based drug

design.

Education

PhD, Biophysics, (Oct 2001- July 2005) Biozentrum, University of

Basel, Switzerland, Advisor : Prof. Tilman Schirmer

Thesis: "Structural investigation into recombinant eye lens Aquaporin-

0 and the BEPS-effector proteins from Bartonella henselae".

M. Phil., Biochemistry, (1998 - 1999) University of Madras, India.

Post graduate diploma, Computer application,(1999), India.

MS, Biochemistry, (1996 - 1998) Annamalai University, India.

BS, Biochemistry, (1992 - 1995) University of Madras, Chennai, India.

Work Experience

Postdoctoral fellow (2007 - present) University of California San

Francisco. (Advisor: Prof. Daniel Minor)

High-throughput screening to identify maximal expression of integral

membrane proteins for structural studies (GFP-fusion).

Postdoctoral fellow (2005 - 2007) Biozentrum, University of Basel,

Switzerland. (Advisor: Prof. Tilman Schirmer)

Structural and functional investigations of the bacterial Type IV

secretion system and novel substrates (BEP proteins) by X-ray

crystallography.

Graduate assistant (2001 - 2005) Biozentrum, University of Basel,

Switzerland. (Advisor: Prof. Tilman Schirmer)

"Structural investigation into recombinant eye lens aquaporin and the

BEPS-effector proteins from Bartonella henselae".

Research assistant (2000 - 2001) (Molecular Biophysics Unit), Indian

Institute of Science, Bangalore, India. (Advisor: Prof. K. Suguna)

Purification and crystallization of aspartic proteases.

Application Specialist (1999 - 2000) CPC Diagnostics Pvt. Ltd.

(Biochemistry and Instrumentation), Chennai, India.

Quality control for diagnostic reagents and product analysis.

Technical Experience

. Expression construct cloning (Restriction-based, Ligation

Independent Cloning (LIC), site-directed mutagenesis)

. Protein overexpression (Escherichia coli and Saccharomyces

cerevisiae)

. Chromatography (FPLC and HPLC systems including Fluorescence Size

Exclusion Chromatography(FSEC))

. Electrophoresis - Blue Native, Western blot and In-gel fluorescence

. Crystallization methods, Microfluidic dialysis and Lipidic Cubic

Phase

. X-ray crystallography (Molecular replacement and experimental

phasing)

. Crystallographic computing (Mosflm, CCP4 package, Coot, Pymol)

. LIMS software

. High throughput screening (GFP whole cell fluorescence assay)

. Light scattering (static and dynamic)

. Spectroscopy (Circular dichroism and Fluorescence)

. Analytical ultracentrifugation

. Technical writing and staff supervision

Publication and Achievements

Palanivelu, D. V., Kozono, D. E., Engel, A., Suda, K., Lustig, A.,

Agre, P. and Schirmer, T. Co-axial association of recombinant eye lens

Aquaporin-0 observed in loosely packed 3D crystals. J Mol Biol. 2006

Jan 27; 355(4):605-611.

Hammon J, Palanivelu D.V., Chen J, Patel C, Minor DL Jr. A green

fluorescent protein screen for identification of well-expressed

membrane proteins from a cohort of extremophilic organisms. Protein

Sci. 2009 Jan;18(1):121-133.

Palanivelu, D.V., Schmid, M.C., Dehio C. and Schirmer T. Crystal

structure of the FIC domain of BepA from Bartonella henselae, the

novel substrate translocated by Type IV secretion system into the

eukaryotes for its pathogenesis (manuscript in preparation).

. Invited speaker: Biozentrum mini-symposium, Switzerland (2006),

"Regional Meeting on X-ray Crystallography", Switzerland (2004),

and "Aquaporin Conference", Switzerland (2002)

. Poster presentations: Spine workshop, Grenoble, EMBL, France

(2008), European Crystallographic Meeting, Hungary (2004), EMBO

conference, Germany (2002), Biozentrum Symposium, Switzerland

(2002, 2003, 2006) and International conference on membrane

proteins, Switzerland (2002)

Awards and Memberships

. Member of Biophysical Society (2010)

. Top academic achievement for Masters candidate (1996-1998)

. Society of Biological chemists (1996 -1998)

References

PhD supervisor

Prof. Tilman Schirmer

Division of Structural Biology,

Biozentrum, University of Basel,

Klingelbergstrasse 70,

CH-4056 Basel, Switzerland

Tel : +41-61-267-**-**

Email: ******.********@******.**

Post-Doc supervisor

Prof. Daniel Minor Jr.

Departments of Biochemistry and Biophysics

Cardiovascular Research Institute

University of California San Francisco,

San Francisco, CA 94158 U.S.A.

Tel : +1-415-***-****

Email: ******.*****@****.***

Research assistant supervisor

Prof. K. Suguna

Molecular Biophysics Unit

Indian Institute of Science

Bangalore-560012 India.

Tel : +91-80-229*****

Email: ******@***.****.*****.**

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