Quality Control Pvt Ltd

SUNIL CHAUDHARI

***** ******** *** 949-***-****

Artesia, CA 90701 ******************@*****.***

Ready to Relocate: Yes Authorize to work in US: Yes

Objective:

My objective is to pursue a scientific career as a lab technician that would enable me to augment my acquired knowledge, bring to forefront my skills and offer opportunities for growth based on achievements

Education:

Master of science, Molecular Biology GPA 3.0/4.0

California state university, Fullerton, USA

Master of Science, Microbiology GPA 3.0/4.0

Sardar Patel University, V.V. Nagar, India Jun ’06

Bachelor of Science, Microbiology GPA 3.0/4.0

Sardar Patel University, V.V. Nagar, India Jun ’04

Related Coursework:

College of Natural Sciences and Mathematics Department of Biology

• Coursework includes Principles of evolution; Lab in Molecular systematics; Molecular Biology; Biochemistry; Bioinformatics; Functional Genomics; Advanced Microbiology; Cell Biology; Seminars in Microbiology; Seminars in Cell Biology

• Practical skilled gained during my degree

PCR, gene cloning, recombination technology, aseptic techniques, bacterial isolation and cultivation

Protein production, isolation and purification, ELISA, immunoassays, spectroscopy, enzyme assays

Aseptic microbiological techniques allowing for the maintenance and growth of bacterial cell cultures, western blot analysis, agarose gel electrophoresis, SDS-PAGE, DNA and RNA extraction, and reagent preparation

Qualitative assays: Western blot, Lipid Strips, Protein Binding assays

Quantitative assay: Liposome study, Computational study

Bioinformatics; Protein BLAST, Nucleotide BLAST, Phylogenetic trees, Tree building methods, Distances, Parsimony criterion, Multiple sequence alignment

• Presentations involve

Lipid binding properties of heat shock proteins

HSPA5 and their lipid binding properties; qualitative and quantitative

Sardar Patel University; Masters of Science in Microbiology

• Coursework included R-DNA Technology; Microbial Physiology; Environmental Biotechnology; Immunology; Enzymology; Biophysics and Instrumentation; Bioprocess and Fermentation Technology; Microbial Biotechnology

• Developed technical abilities in DNA and Plasmid Isolation, Transformation in bacterial cells; Protein Chemistry; Chromatography (TLC); Protein isolation and partial purification of alkali tolerant Xylanase

• Conducted seminars and gave research paper presentations

Sardar Patel University; Bachelors of Science in Microbiology

• Developed basic Microbiological Laboratory and Analytical Techniques; Qualitative and Quantitative Analysis of Carbohydrates, Proteins and Lipids; Serology, Hematology, Clinical Biochemistry, Antibiogram related assays

Relevant Experience:

Researcher (Jan 2008 – Present)

• Project Title: “Characterization of the lipid binding properties of HSPA5”

• Description: Responsible for bacterial cell culture of Escherichia Coli BL21, for use in the expression and purification of Heat shock proteins. Used protein purification techniques to purify the protein for Quantitative and qualitative assays.

• Under the guidance of Dr. Nikolas Nikolaidis, PhD., College of Natural Sciences and Mathematics Department of Biology, CSUF, Ca, USA.

Researcher (Mar 2004 – Jun 2006)

• Project Title: “Cellulase free Xylanase production by locally isolated alkali tolerant Bacterial strain E-10-1 and its Application on pulp Bleaching”

• Description: I performed isolation and characterization of Bacillus group of organisms; did a comparative study between the natural and commercial Xylnase producing isolates, isolation and cultivation of isolates, conducted partial purification and characterization of Xylanase to determine Xylanase activity on commercial and laboratory made pulp.

• Under the guidance of Dr. Amita R. Shah, PhD., Sardar Patel University, V V Nagar, India.

Research support:

Research Grant - Associated Students, Inc, California State Uiversity, Fullerton (2011)

Professional Experience:

Denis Chem Lab Pvt Ltd. (August 2007 to September 2007)

Quality Control Microbiologist

• Perform microbiological and sterility testing for release of raw materials and finished products according to approved procedures.

• Bacterial Endotoxin Testing (BET) / Pyrogen testing (LAL test)

• Stability Test

• Aseptic/Microbial monitoring

• Maintain records of test data per cGMP.

• Develop and assist in validation of test methods and technology.

• Perform investigational testing and other duties as assigned by supervisor.

• Maintain inventory for use in microbiological testing.

• Perform harmonization according to IP, USP, and BP regulations

• Set up and prepare field room

Software skills

• Internet Applications

• Microsoft Office Suite (word, excel)

• Windows XP/Vista/Apple IOS

Contact this candidate