Assistant Electrical

Darren J. Bauer

*** ****** **** ******, **, ***** 603-***-**** *******@***.***

Profile

Motivated, personable professional with exceptional technical skills and knowledge developed

from a diverse background in molecular biology and the electrical and electromechanical fields.

Works well independently, yet is effective as part of a team.

Skills Summary

RNA, DNA & Protein Two Dimensional Gel Next Generation sequence

isolation and purification Electrophoresis analysis

cDNA library Gene expression analysis R Programming

construction

Programmable Logic Analytical Troubleshooting

PCR Controllers

Experience

University of New Hampshire, Durham, NH, August 2002 to Present

Graduate Research Assistant Conduct research for dissertation project as well as research on NSF funded

grant to elucidate the nematode branch of the tree of life

Graduate Teaching Assistant Lead discussions and answer student questions regarding lecture.

Demonstrate lab techniques. Assign, explain and grade homework and lab reports. Grade

exams

University of Missouri, Kansas City, MO, June 2000 to May 2002

Undergraduate Laboratory Assistant – Routine lab preparation duties

U.S. Environmental Protection Agency, Kansas City, KS, May 2001 to September 2001

Summer Intern – Maintain Daphnia cultures for aquatic toxicity tests. Routine lab preparation

duties

Physicians Reference Laboratory, Overland Park, KS, May 1999 to May 2001

Laboratory Assistant – Receive, accession and aliquot clinical and forensic samples for testing

Genex, Beatrice, NE, March 1996 to September 1996

Electromechanical Technician – Electrical and mechanical service work and installation

Siemens Energy and Automation, Elk Grove Village, IL, September 1995 to March 1996

Field Service Engineer – Troubleshoot and repair of all Siemens automated machine tool products

NEAPCO, Beatrice, NE, March 1993 to September 1995

Electromechanical Technician – Maintain, troubleshoot and repair automated machine tools

Commonwealth Electric of the MidWest, Lincoln, NE, January 1993 to March 1993

electrical installation

Apprentice Electrician – Commercial

Education

University of New Hampshire, Durham, NH

Ph.D. in Genetics, Anticipated: June 2010

University of Missouri, Kansas City, MO

B.S. in Biology, May 2002

Southeast Community College, Beatrice, NE

A.S. with Biotechnology emphasis, December 1998

Southeast Community College, Milford, NE

A.A.S. in Electrical Technology, December 1992

Southeast Community College, Milford, NE

A.A.S. in Electromechanical Technology, June 1992

Publications

Smejkal G.B., and D.J. Bauer. 2-D in a Day: A Two-Dimensional Gel Electrophoresis Work Flow

Completed in Under Five Hours. American Biotechnology Laboratory, (Accepted)

Bauer D. J., W.K. Thomas. Identification of Daphnia genes involved in resistance to toxic Microcystis

aeruginosa using RNA-Seq. (In Prep)

Colbourne J.K., M.E. Pfrender, D. Gilbert, W.K. Thomas, J.H. Choi, A. Aerts, G. Arnold, M.K.

Basu, D.J. Bauer, et. al. The Genome Sequence of the Crustacean Daphnia pulex. (In Prep)

Bauer D.J. and W.K. Thomas. The Glutathione S-Transferase Supergene Family in Daphnia. (In Prep)

Bauer D.J., G.B. Smejkal, W.P. Kuo and W.K. Thomas. Differential Protein Expression Detected

by 2DGE of Individual Daphnia. (Submitted)

Dong Q., E. Lindquist, Z. Tang, J. Shaw, D.J. Bauer, Z. Smith, R. Patwardhan, M. Wang, M.

Pfrender, J. Andrews, J.W. Hamilton, W.K. Thomas, J. Boore and J.K. Colbourne. The

Daphnia transcriptome under environmental stress, based on 150,000 Expressed Sequence

Tags (Submitted)

Colbourne J.K., B.D. Eads, J. Shaw, E. Bohuski, D.J. Bauer, and J. Andrews. Sampling Daphnia’s

expressed genes: preservation, expansion and invention of crustacean genes with reference

to insect genomes. BMC Genomics, 2007. Jul 6; 8:217

Poynton H.C., J.R. Varshavsky, B. Chang, G. Cavigiolio, S. Chan, P.S. Homan, A.V. Loguinov, D.J.

Bauer, K. Komachi, E.C. Theil, E.J. Perkins, O. Hughes and C.D. Vulpe. Daphnia magna

ecotoxicogenomics provides mechanistic insights into metal toxicity. Environmental Science and

Technology 2007 Feb 1; 41(3): 1044-50.

Colbourne J.K., E. Lindquist, D. Bauer, P. Brokstein, W.K. Thomas and J. Andrews. Gene

discovery in Daphnia by expressed sequence tag sequencing. Integrative and Comparative Biology

2005 Dec; 45 (6): 979.

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