Manufacturing Assistant

Jingchun Yang

**-** *** ********* ** Cell phone: (215) 435-

0398

East Windsor, NJ 08520 E-Mail:

**********@*****.***

OBJECTIVE A research scientist position in pharmaceutical industry working on innovative and

challenging projects, where analytical and creative talents will have valuable

application.

BACKGROUND More than 8 years of experience in pharmaceutical research and development.

SUMMARY Demonstrated strengths in developing procedures and problem-solving. A flexible

team player with a reputation for maintaining the highest possible quality and

productivity. Expertise includes:

Molecular Biology/Cell-based Assays

Biochemical assays/Immunology

Hit to lead optimization

High and ultra high throughput screening

Instrumentation/Computer

PROFESSIONAL EXPERIENCE

SUMMARY OF INDUSTRY EXPERIENCE

Ligand Pharmaceuticals Inc. (Pharmacopeia Drug Discovery Inc.), Princeton, NJ

(July, 2001 – December, 2009)

Molecular Biology/Cell-based Assays

Utilized standard mammalian cell (CHO, HEK-293E, Hela and Jurkat), E.coli and yeast cell

culture techniques

Introduced DNA into mammalian cell by transfection through calcium phosphate and

lipofectamine techniques

Established stable or transit transfected mammalian cell lines

Developed and optimized 1536-well luciferase reporter assay and β-arrestin recruitment assay

Developed and optimized 384 and 1536-well HTRF cAMP assay

Developed and optimized 384 and 1536-well calcium mobilization FLIPR assay for screening

GPCR and ion-channel targets

Developed and optimized WGA-SPA assay, membrane filtration assay, whole cell binding

assay and 35S-GTPγ S binding assay to confirm small molecule binding affinity

Biochemical assays/Immunology

Developed and optimized 384 and 1536-well IMAP (Immobilized Metal Assay for

Phosphochemicals) and HTRF assays for screening kinase inhibitors

Developed and optimized 384 and 1536-well AlphaScreen assay for screening kinase

inhibitors

Established enzyme-linked immunosorbent assay (ELISA) for the measure of cytokines and

chemokines

Hit to lead optimization

Developed and conducted various cell-based and biochemical assays

Participating in characterization of small molecule leads in support of structure activity

relationship studies

Analyzed and summarized experimental data using a variety of computer software

High and ultra high throughput screening

Developed and optimized cell-based and biochemical assays to support high

throughput screening

Screened a number of compounds using the assays in 384 and 1536-well formats

Page2 Jingchun Yang

Expertise in operating lab equipments and knowledge of different screening formats in both

enzymatic and whole cell systems

Instrumentation/Computer

FLIPR384 and FLIPRTETRA (Molecular Devices), ViewLux ultraHTS Microplate Imager

(PerkinElmer), EnVision Multilabel Plate Reader (PerkinElmer), Victor Multilabel Plate Reader

(PerkinElmer), Acquest Multimode Plate Reader (Molecular Devices), Biomek FX Laboratory

Workstation (Beckman Coulter), Biomek 2000 Laboratory Automation Workstation (Beckman

Coulter), MicroBeta TriLux (Wallac), Matrix PlateMate Automated Pipettor (Cybio) and

Automated 1536-well Non-contact Dispenser

Proficiency in Visual C++, LabView programming, Word, Excel, Powder Point, Graph Pad

Prism, DNAstar/Lasergene

Department of Bioscience & Biotechnology, Drexel University, Philadelphia, PA

(October, 1999-June, 2001)

Cloning and characterization mouse RPA4 gene

Achieved by using PCR & nested PCR amplification, cDNA library screening, southern and

northern blotting techniques

Effects of Adriamycin and antioxidant on HeLa & mouse cardiac cell mitochondrial DNA

Achieved by using tissue cell culturing, LDH cell cytotoxicity assay, cellular DNA purification

and southern blotting technique

RPA4 expression in human normal and ovarian cancer tissues

Achieved by using SDS-polyacrylamide gel electrophoresis and western blotting technique

Optimizing conditions for the purification of anti-RPA4 antibody

Achieved by using recombinant bacterial expression system, GST-tag protein purification and

antigen affinity column purification

Sichuan Pharmaceutical Plant, Sichuan, China (1991-1998)

Antibiotic manufacturing and development

Optimization of medium formulation and fermentation conditions for antibiotic manufacturing

Antibiotic manufacturing management and industrial problem solving

TEACHING EXPERIENCE

Teaching assistant in Department of Bioscience & Biotechnology, Drexel University (1999-

2001)

Taught and prepared for lab courses: Advanced Cell Biology, Applied Biology, Nutritional

Chemistry, Food/Nutrient Analysis

EDUCATION

June 2002 MS Department of Bioscience & Biotechnology, Drexel University, Philadelphia,

PA

July 1991 BS Department of Bioengineering, Sichuan University, Sichuan, China

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