Engineer Assistant

Curriculum Vitae

Qing Liu

Personal data:

Name: Qing Liu

Nationality: China

Visa situation: EAD (Green Card Pending, permitted to work)

Present address: **** ********** **. *** *

Oceanside CA 92056, USA

Phone: 413-***-**** (cell)

E-mail: *********@*****.***

Education and Professional Experience

Jan. 2005 to Sep. 2009: Senior Research Assistant in the Department of

Biochemistry and Molecular Biology, under supervision of Prof. Maurille J.

Fournier, University of Massachusetts, Amherst, U.S.A.

Feb. 2002 to Jul. 2004: Research assistant in Molecular Biology, under

supervision of Prof. Shulamit Michaeli, Bar-Ilan University, Ramat Gan,

Israel.

Oct. 1988 to Jan. 2002: Engineer, In the Data Processing Center,

Environmental Protection Bureau, Handan, 056032. Hebei Province, China.

Sep. 1984 to Jul. 1988: Undergraduate student in Henan University. Computer

Application. KaiFeng, Henan Province, China.

Research experience

1) Examining the effects of depleting rRNA nucleotide modification on

ribosome biogenesis and function in yeast.

2) Determining how a processing-modification dual function small nucleolar

RNA (snoRNA) acts on pre-rRNA in yeast.

3) Indentifying novel small nucleolar RNAs in trypanosomes, in a genome

wide basis.

4) Developing an approach to down-regulate snoRNA expression in

trypanosomes.

5) Determining the roles of sequences flanking snoRNA coding region in the

biogenesis of snoRNAs in trypanosomes.

6) Identifying and characterizing protein factors required for pre-mRNA

splicing in trypanosomes.

Research skills

1. Molecular Biology and Biochemistry: DNA and RNA isolation, cDNA and

genomic DNA cloning, PCR, RT-PCR, plasmid preparation, plasmid

construction, gel electrophoresis, Southern and Northern blotting, DNA and

RNA sequencing, RNA structure probing, primer extension, RNA modification

detection, in vivo pulse-chase labeling, sucrose gradient fractionation,

ribosome profile, protein tagging, Western blotting.

2. Molecular Genetics: mutagenesis, DNA recombination, gene expression

regulation (knock-out or knock down, by inducible promoter or RNAi).

3. Cell Biology: Transformation, stable and transient transfection of

cells, including bacteria, trypanosomes, and yeast cells.

4. Immunology: immunoprecipitation; immunofluorescence; affinity

purification.

Publications

1. Liang, XH., Liu, Q., Liu, QS., King, T., and Fournier, M.J. Strong

cooperativity between functional domains in a dual-function snoRNA infers

coupling of rRNA processing and modification events. 2010. Nucleic Acid

Research, Accepted.

2. Liang, XH., Liu, Q., and Fournier, M.J. Loss of rRNA modifications in

the decoding center of the ribosome impairs translation and strongly delays

pre-rRNA processing. RNA. 2009, 15(9):1716-1728

3. Liang, XH., Liu, B., Piekna-Przybylska, D., Liu, Q., and Fournier, M.J.

Mis-targeted methylation in rRNA can severely impair ribosome synthesis and

activity. RNA Biol. 2008, 5(4) 249-254.

4. Liang, XH., Liu, Q., and Fournier, M.J. rRNA Modifications in an

Intersubunit Bridge of the Ribosome Strongly Affect both Ribosome

Biogenesis and Activity. Mol. Cell. 2007, 28:965-977.

5. Liang, XH., Liu, Q., Liu, L., Tschudi, C., and Michaeli, S. Analysis of

spliceosomal complexes in Trypanosoma brucei and silencing of two splicing

factors Prp31 and Prp43. Mol. Biochem. Parasitol. 2006, 145:29-39.

6. Liu, Q., Liang, XH., Uliel, S., and Michaeli, S. Identification and

characterization of Lsm proteins in trypanosomes. J. Biol. chem. 2004,

279:182**-*****.

7. Liang, XH., Ochaion, A., Xu, YX., Liu, Q., and Michaeli, S. snoRNA

clusters in trypanosomatid Leptomonas collosoma: genome organization,

expression studies, and the potential role of sequences present upstream

from the first repeated cluster. J. Biol. Chem. 2004. 279:5100-5109.

8. Liang, XH., Liu, Q., and Michaeli, S. snoRNAi-small nucleolar RNA

interference induced by anti-sense or double-stranded RNA in

trypanosomatids. 2003. Proc. Natl. Acad. Sci. USA, 100:7521-7526.

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