Medical School

JEAN LOUISE SPENCE, Ph.D.

Home Address **** ******* ******

San Diego, California 92123

Home Phone Number 858-***-****

e-mail ********@***.**.***

SUMMARY

Computer skills: Perl scripting, SQL, SQL-PL, database construction in

Oracle11g

. Technical Writing: grant applications, manuscripts, patent

applications, poster presentations

. Molecular Biology: Immnunoblots, microarrays, DNA/RNA extracting, DNA

sequencing, PCR, QPCR, protein purification, mammalian cell culture,

polysome preparations

. Biochemistry: Protein purification, HPLC, FPLC

MAJOR ACCOMPLISHMENTS

. Developed a novel approach for constructing disease-related networks

by identifying novel motifs or regions of identity through

bioinformatic analysis of untranslated regions.

. Identified mRNAs that are under differential translational control in

transformed cells by microarray analysis of total and polysome-

associated mRNAs.

. Discovered that ribosomes are modified with lysine-63 linked

multiubiquitin chains at the petidyl transferase center and that this

ubiquitination event facilitates translation in yeast.

. Discovered a non-canonical Lysine 63 linked multiubiquitin chain in

yeast that is required for UV induced mutagenesis and DNA repair.

PROFESSIONAL EXPERIENCE

Senior Research Scientist

Omnitron Biosciences, San Diego, CA

2003-Present

. Currently collaborating with Scripps Genomics Medicine on an STTR

project involving a novel bioinformatics approach to neurodegenerative

diseases.

. Published two manuscripts as communicating author.

Adjunct Staff

2007-2008

Southwestern Community College

Consultant

2006

Arrayomics, San Diego

. Performed literature and prior art searches of patent databases

Senior Research Scientist

Sidney Kimmel Cancer Center, San Diego, CA

2000-2003

Research Associate

Scripps Research Institute

2000

Research Scientist

UCSD, Department of Pharmacology 1998-

2000

Postdoctoral Fellow

University of Rochester, Rochester, New York

1997-1998

Postdoctoral Fellow

University of California at San Diego, Pharmacology Department

1995-1997

Postdoctoral Fellow

Harvard Medical School, Boston, Massachusetts

1989-1995

Postdoctoral Fellow

Massachusetts Institute of Technology, Cambridge, Massachusetts.

1988-1989

EDUCATION

Ph.D. University of Utah

M.S. Microbiology, The University of Texas at Austin.

B.S., cum laude University of Florida, Gainesville, Florida.

RESEARCH AWARDS

1994. American Cancer Society, Senior Postdoctoral Fellowship,

Harvard

Medical School.

1991-1992 Epply Foundation Grant, Harvard Medical School

American Cancer Society Postdoctoral Fellowship, Harvard Medical

School

1989. American Cancer Society Postdoctoral Fellowship, Massachusetts

Institute of Technology

1983-1988 N.I.H. Predoctoral Training Grant, University of Utah

1976-1979 N.I.H. Predoctoral Training Grant, The University of Texas

at Austin

PUBLICATIONS

Spence, J.L. Pathway prediction by bioinformatic analysis of the

untranslated regions of the CFTR mRNA. 2009. Genomics 94:39-47

Spence, J.L., C. Eckhart, B. Duggan, M. McClelland, and D. Mercola. 2006.

Messenger RNAs Under Differential Translational Control in Ki-ras

Transformed Prostate Epithelial Cells. Molecular Cancer Research 4:47-61.

Spence, J., R. Gali, G. Dittmar, F. Sherman, M. Karin, and D. Finley.

2000. Cell Cycle-Regulated Modification of the Ribosome by a Variant

Multiubiquitin Chain. Cell 102: 67-76.

Spence, J., S. Sadis, A.L. Haas, and D. Finley. 1995. A Novel

Multiubiquitin Chain Implicated in DNA Repair. Mol. Cell. Biol. 15: 1265-

1273.

Spence, J.L., A. Cegielska, and C. Georgopoulos. 1990. Role of the

Escherichia coli Heat Shock Proteins DnaK and HtpG (C62.5) in the Response

to Nutritional Deprivation. Journal of Bacteriology 172: 7157-7166.

Tilly, K., J.L. Spence, and C. Georgopoulos. 1989. Modulation of the

Stability of the Escherichia coli Heat Shock Regulatory Factor sigma 32.

Journal of Bacteriology 171:1585-1589.

Spence, J.L. and C. Georgopoulos. 1989. Purification and Properties of

the Escherichia coli Heat Shock Protein HtpG. 1989. Journal of Biological

Chemistry 264: 4389-4403.

Ferrari, F.A., K. Trach, D. Lecoq, J. Spence, E. Ferrari, and J.A. Hoch.

1985. Characterization of the spoOA Locus and its Deduced Product. Proc.

Nat. Acad. Sci. U.S.A. 82: 2647-2651.

Schmitt, M.P., P.J. Beck, C.A. Kearney, J.L. Spence, D. DiGiovanni, J.P.

Condreay, and I.J. Molineux. 1987. Sequence of a Conditionally Essential

Region of Bacteriophage T3, Including the Primary Origin of DNA

Replication. Journal of Molecular Biology 193: 479-495.

Molineux, I.J. and J.L. Spence. 1984. Virus-Plasmid Interactions: Mutants

of Bacteriophage T3 that Abortively Infect Plasmid F-Containing (F+)

Strains of Escherichia coli. Proc. Nat. Acad. Sci. U.S.A. 81: 1465-1469.

Spence, J.L., P.Q. Mooney, and I.J. Molineux. 1983. Physiological

Properties of a T7-T3 Recombinant Phage that Productively Infects Strains

of Escherichia coli that Harbor the F Plasmid. Journal of Virology 46: 895-

900.

Molineux, I.J., P.Q. Mooney, and J.L. Spence. 1983. Recombinants Between

Bacteriophages t7 and T3 Which Productively Infect F-Plasmid Containing

Strains of Escherichia coli. Journal of Virology 46: 881-894.

Rup, B.J., J.L. Spence, J.D. Hoelzer, R.B. Lewis, C.R. Carpenter, A.S.

Rubin, and H.R. Bose. 1979. Immunosuppression Induced by Avian

Reticuloendotheliosis Virus: Mechanism of Induction of the Suppressor

Cell. J. Immunol. 123: 1362-1369.

Scofield, V.L., J.L. Spence, W.E. Briles, and H.R. Bose. 1978.

Differential Mortality and Lesion Responses to Reticuloendotheliosis Virus

Infection in Marek's Disease Resistant and Susceptible Chicken Lines.

Immunogenetics 7: 169-172.

PRESENTATIONS

Spence, J.L. 2009. Pathway prediction by bioinformatic analysis of the

untranslated regions of the CFTR mRNA. Presented at the 4th Annual Systems

to Synthesis Symposium, San Diego.

Spence, J.L. 2009. Pathway prediction by bioinformatic analysis of the

untranslated regions of the CFTR mRNA. Selected for presentation at the

2009 Illumina Users Group Meeting.

Spence, J. 2005. Identification of Highly Conserved Motifs in the 3'UTRs

of mRNAs Encoding Cell Cycle Regulators and Related Proteins. Presented at

the 4th Salk Institute Cell Cycle Meeting, La Jolla, California.

Spence, J. and D. Mercola. 2001. Differential Ubiquitination of Ribosomes

from Transformed and Normal Prostate Epithelial Cells. Presented at the

Salk Institute/ EMBL meeting on Oncogenes and Growth Control, Salk

Institute, La Jolla, California.

Spence, J. and D. Mercola. 2001. Differential Ubiquitination of Ribosomes

from Transformed and Normal Prostate Epithelial Cells. Presented at the

International Conference on Gene Therapy of Cancer, Coronado, California.

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