Project Quality Assurance

Domenico Alessio Panzica

Date and Place of Birth: 11th June 1984, Caltanissetta, Italy

Marital status: Single

Address UK: 30 E St. Andrew Street, AB25 1JA Aberdeen,

Scotland UK

Address USA: 1211 W. Glenn Lane Mt.

Prospect, IL 60056

Email address: *************@*******.**

Mobile telephone UK: 075********

Mobile telephone USA: 847-***-****

Home USA: 847-***-****

Education:

2009 MSc Instrumental Analytical Sciences: DNA Analysis, Proteomics and

Metabolomics with Distinction.

The Robert Gordon University

MSc project: ''The study into the role of Zn compounds in the

inhibition of inflammation". The project was carried out at The

Rowett Research Institute of Nutrition and Health under the

supervision of Dr Charles Bestwick (Aberdeen University, The Rowett

Research Institute of Nutrition and Health) and Dr Paul Kong Thoo

Lin (The Robert Gordon University).

Module studied:

Professional Skills Quality Assurance, Chemometrics (A);

Spectroscopy (B); Separation, Electroanalysis and Microscopy (B);

Laboratory Work (B); Contemporary Techniques / Analytical problem

solving 'Expression and characterisation of Housekeeping genes by

protein analysis studies'. Project under supervision of Dr. Paul

Kong Thoo Lin(A); Extensive Elective Studies, DNA analysis,

Proteomics and Metabolomics (B); Project preparation (A); Research

project (A).

2003- 2008 BSc in Biological Sciences: applied molecular biology

(grade 110/110 cum laude. Equivalent grade: 1st class ). Universit

Degli Studi di Palermo, Palermo, Italy.

A broad knowledge in basic sciences (i.e. maths, physics, and

chemistry) was acquired along with general biological sciences

studies (i.e. cell biology, genetics, biochemistry, microbiology,

plant biology, zoology, physiology, immunology, etc.).

2006-2007 Erasmus student (European exchange programme), at The

Robert Gordon University of Aberdeen, Scotland.

Module studied at the host University:

Physical Chemistry, Genetic engineering and Biotechnology,

Bioanalytical Techniques, Laboratory investigation of Biomolecules

and Metabolism.

Throughout the exchange programme I carried out my honours research

project at the host university (Title: The effect on gene expression

of polyamine metabolic enzymes in drug treated cancer cells).

2000-2003 High school Diploma "School of commerce" Istituto Tecnico

Commerciale "M. Rapisardi", Caltanissetta, Italy.

1998-2000 School of Biological Sciences Istituto Tecnico per

Attivita' Sociali, "L. Russo", nCaltanissetta, Italy.

Research and other laboratory experience

Mar 2009- Aug 2009 MSc Research Project Rowett Research Institute of

Nutrition and Health, University of Aberdeen / The Robert

Gordon University. Supervisors: Dr. Charles Bestwick/ Dr. Paul

Kong Thoo Lin. (Internship)

The potential therapeutic

effect of a novel Zn macrocyclic complex was investigated on

colon fibroblasts CCD-18Co and RAW 264.7 macrophages after

stimulation with menadione to induce reactive species

production.

Techniques: Aseptic

technique; Cell culture; MTT and Trypan Blue assays; Flow

Cytometric analysis (FACS) and Enzyme-linked immunosorbent

assay (ELISA).

Jan - Feb 2009 DNA Analysis, Proteomics, Metabomolics Elective, The

Robert Gordon University.

Detection of the housekeeping gene Actin by protein analysis

studies.

The aim of this study was to suggest and design an assay that

was suitable to assess Actin at protein level in cancer cells.

Total protein was extracted from cells and Actin expression

detected by Western blot analysis.

Techniques: Aseptic technique; Cell culture; protein

extraction; Lowry method; SDS-PAGE electrophoresis; Western

Blot; wet transfer.

Mar - Jun 2007 BSc Honours research Project. The Robert Gordon

University, Aberdeen, Scotland. Supervisors: Dr. Paul Kong Thoo

Lin/ Dr. Giovanna Bermano

Polyamines analogues have been demonstrated to exhibit anti-

tumour activity in a large number of tumours. The study aimed

to test the toxicity of bisnaphthalimidopropyl polyamine

derivative compounds (BNIPDadec and BNIPDaoct) in a breast

cancer cell line (MDA MB 231) to assess which one was most

active in reducing the progression of cancer cells.

In addition the expression of genes involved in polyamine

anabolism and catabolism was assessed by RT-PCR in MDA MB 231

cells treated with BNIPDadec and BNIPDaoct.

Techniques undertaken: Cell Culture; Aseptique tecquiniques;

MTT assay; RNA extraction; RT- PCR; Agarose Gel

Electrophoresis.

Theoretical background:

I have theoretical knowledge in fields of:

Biotechnology, Inorganic and Organic Chemistry, Biochemistry,

Microbiology, Molecular Biology, Immunology, Modern vaccines,

Cell and Tissue cultures, Genetics and genetic engineering,

Enzymology, Physiology, Anatomy, Physic, Ecology, Cytology and

Histology, Microscopy, Botanic, Plant Physiology, Developmental

Biology, Separation Techniques, DNA analysis, Proteomics,

Metabolomics, Chemometrics, Spectroscopy.

Technical Skills and Biochemical Techniques:

I have experience in a number of techniques:

Aseptic Technique HPLC

RNA and DNA extraction Flow

cytometry

Protein extraction

ELISA

Cell culture

Restriction enzyme digestion

Tissue culture

Transformation

MTT

Chromatography

Trypan Blue assay

PAGE/SDS-PAGE

PCR /RT PCR

Microscopy

DNA gel electrophoresis Western

Blotting

Cloning

Lowry method

General skills:

Computer skills: Computer maintenance, internet skills (bibliographic

search);

Software: MS Office Pack, QuantityOne (gel analysis and

quantification), CellQuest (Flow Cytometry analysis), MiniTab

(statistical analysis).

Languages: Italian

Native

Spoken and written English Fluent

French

Intermediate

Spanish

Intermediate

Driving licence: Full, clean driving licence

Hobbies:

I like to go to the gym, travel and socialise with others. I enjoy

team work activities, both academic and social.

Referees:

Dr. Paul Kong

Dr.Charles Bestwick

Reader/Senior Lecturer

Hon Reader/Senior Scientist

Postgraduate Course Leader

The Rowett institute of Nutrition and Health

The Robert Gordon University

Microbial Biochemistry/Gut Health division

School of Pharmacy and Life Sciences

Greenburn Road, Bucksburn

St. Andrew Street

Aberdeen AB21 9SB

Aberdeen AB25 1HG

Scotland UK

Scotland UK

E-mail: *.*.*.****-****-***@***.**.**

E-mail: *.********@****.**.**

Tel: (+44)-(0-122*-******

Tel: +44 (0-122*-****** ext: 2477

Dr. Giovanna Bermano

Dr. Anne McPherson

Theme leader

Lecturer

Cardiovascular & Metabolic Disease Research

Faulty of Health and Social Care

Institute for Health & Welfare Research

The Robert Gordon University

St. Andrew Street

St. Andrew Street

Aberdeen AB25 1HG

Aberdeen AB25 1HG

Scotland UK

Scotland UK

E-mail: *.*******@***.**.**

E-mail: *.*.*********@***.**.**

Tel: + 44 (0-122*-******

Tel: + 44 (0-122*******

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