Manager Technician

Rob W. Herman

***** ******* **

San Diego, CA ***29

310-***-****

********@*****.***

Highlights and Skills

. Primer design and site-directed mutagenesis by overlap extension and

Quik-change PCR

. Standard molecular cloning and subcloning techniques

. E. coli transformations and screening for recombinant clones by

electrophoresis and DNA sequencing

. Sequencing with ABI3100 and sequence analysis using software such as

Chromas, Vector NTI and TeraTerm

. RNA isolation, purification and in vitro transcription

. Membrane protein expression in bacterial systems by fermentation and

transient transfection in mammalian cell cultures

. Membrane and protein isolation and purification via differential

centrifugation and affinity columns

. Trained to work in BSL 2 laboratories under aseptic conditions

Trained to handle mice; intraperitoneal injections, cardiac puncture,

euthanization

Fluorescence microscopy and analysis using Metamorph software

Professional Experience

Seattle Biomedical Research Institute, Seattle, WA

Research Technician II (January 2009-Present)

Flow cytometry and immunofluorescence assays to quantify malaria parasite

infectivity when exposed to specific drug concentrations.

Maintenance of malarial infection in mice via infected blood transfers.

Voluntary Leave of Absence (December 2006-December 2008)

Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA

Research Associate II (June 2005-November 2006)

. Design and generation of mutant plasmids of proteins using site

directed mutagenesis.

. Protein expression using mammalian cell culture (transient

transfection).

. Purification of detergent-solubilized proteins using immunoaffinity

columns.

. Purification of native proteins from tissue using affinity columns

and differential centrifugation.

. Spin labeling of proteins for an EPR spectroscopy project focused

on determining inter-molecular distance measurements.

Scripps Institution of Oceanography, UCSD Marine Biology Division

Research Associate II (June 2004-April 2005)

. Extraction of RNA, DNA and proteins from wild type and mutant

cyanobacteria Synecococcus for microarray analysis, focusing on the

response from physiological and environmental stresses during

growth.

. Generation of gene knockouts and mutants using site-directed

mutagenesis.

. Laboratory safety coordinator and purchasing agent.

Department of Physiology, UCLA School of Medicine, Los Angeles, CA

Research Associate I (October 2001-March 2004)

. Design and generation of mutant genes via site-directed mutagenesis

and insertion into plasmids for senior members in a laboratory

focused on structure-function relations in voltage-gated ion

channels and transport proteins.

. Transcription and purification of mRNA from mutant clones for

microinjection into Xenopus oocytes.

. Preparation of DNA constructs and clones for mammalian cell

transfection.

National Genetics Institute, Los Angeles, CA

Laboratory Technician (May 2001-October 2001)

. Viral testing procedures of biological specimens.

. Trained in BSL 2 laboratories that specialized in quantification of

viral samples (HIV, HPV, Hep A, B and C and others).

Department of Chemistry, Syracuse University and University of

Connecticut

Laboratory Specialist (August 1998- March 2001)

. Manager of the Protein Expression and Isolation Laboratory.

. Membrane protein expression using mid-scale fermentation

bioreactors (10 L).

. Membrane-bound protein isolation and purification by differential

centrifugation and sucrose density gradients.

. Co-directed move of research facilities from Syracuse University to

University of Connecticut.

. Designed and developed a large-scale fermentation system using a

series of bioreactors (>50 L) to express native and mutant

proteins.

. Renovations and design of six laboratories in the University of

Connecticut, including a fully functional and contained Protein

Expression and Isolation Laboratory with a built-in autoclave and

large-scale bioreactor system.

. Purchasing agent for the research group.

Education

B.A. Earth Sciences, Adrian College, Adrian, MI, 1995

References available upon request.

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