Supervisor Project
Resume:
Michelle A. Gonzales-Pacheco, B.S.
Lakeside, CA 92040
Phone: 619-***-****
Cellular: 619-***-****
Email: **************@*****.***
Objective:
To be a member of a team focused on innovative research in the fields of
genetics, biology and genomics in a stimulating biotechnology or academic
environment.
Education
B.S. 2002. California State University, Fresno, CA. Molecular and
Cellular Biology
Professional Experience
Senior Research Associate, Tanabe Research Laboratories, San Diego, CA.
2007- 2010
Performed research for preclinical drug discovery programs involving
molecular cloning and recombinant expression of target proteins for high-
throughput screens, enzymology and binding assays
Provided stably and transiently transfected cell lines for screening and
proof-of-concept experiments
Developed and validated reporter gene transcriptional assays for an orphan
nuclear receptor project
Responsible for the maintenance of multiple mammalian, insect and bacterial
cell lines
Performed qPCR analysis for proof-of-concept and biomarker analysis
experiments
Wrote standard operating procedure (SOP) documents for project transfers
Supervisor: Dr. Michael Fitch
Research Technician II, Sidney Kimmel Cancer Center, San Diego, CA.
2004- 2007
Performed research to assist in the identification of antibodies
targeting the vasculature of normal and tumor tissues
Cloned and expressed recombinant proteins
Cultured and subcloned hybridoma cells; produced and purified monoclonal
antibodies
Performed Western blot and qPCR analyses to validate tissue distribution of
candidate vascular protein markers
Supervisor: Dr. Jacqueline Testa
Research Associate, Selective Genetics, San Diego, CA.
2002- 2004
Performed novel research using bacteriophage display libraries for
targeting and transduction of mammalian carcinoma cell lines.
Assisted in the development of phage combinatorial libraries for the
display of complex cDNA ligands from mammalian sources.
Performed selection of novel cDNA ligands from phage libraries using in
vitro immunoassays.
Optimized selection methods to assess potential hits in cell-based assays.
Produced and qualified phage library preparations.
Assisted in in vivo targeting and selection of internalized ligands using
phage display libraries.
Supervisors: Dr. Michael Burg, Dr. Peg Faix
Researcher, Yale University, New Haven, CT.
(Summer 2002)
Research Topic: Functional genomics in plants including mutation
detection, expression profiling and cloning.
Supervisor: Dr. Stephen Dellaporta
Student Independent Research, California State University, Fresno, CA.
(January-May 2002)
Research Topic: Participated in project to monitor expression of cell
death genes using in situ hybridization. Various activities include
tissue fixation, slides for in situ hybridization, and PCR analysis.
Supervisor: Dr. Alejandro Calderon-Urrea
Technical Experience
Molecular Biology: Standard cloning techniques, DNA preparation, RNA
isolation, cDNA amplification, qPCR (ABI 7900HT), ELISA, Western and
Southern blotting, in situ hybridization, PCR
Protein Purification: Baculolovirus, mammalian and bacterial protein
expression. IMAC protein purification, protein A affinity purification,
BCA analysis, WAVE Bioreactor, AKTA Explorer 100 instrumentation.
Tissue Culture: Maintenance of adherent and suspension cell lines,
transfection, stable cell line selection, cell surface staining for flow
cytometry
Lab Automation: Eppendorf epMotion, BioTek Microflo dispenser, Molecular
Dynamics Analyst GT plate reader
Other: Tissue fixation, embedding and sectioning, in vivo biolocalization
models
Software: DNAstar, Microsoft Office, Graphpad Prism
Publications:
Faix, PH, Burg, MA, Gonzales, M, Ravey, EP, Amburn, E, Baird, A, Larocca,
D. Phage display of cDNA libraries: enrichment of cDNA expression
using open reading frame selection. BioTechniques 36:1018-1029, June
2004.
Burg, M, Ravey, EP, Gonzales, MA, Amburn, E, Faix, PH, Baird, A, Larocca,
D. Selection of internalizing ligand-display phage using rolling circle
amplification for phage recovery. DNA Cell Biology 23: 457-62, July 2004.
Abstracts:
Faix, PH, Burg, M, Ravey, EP, Gonzales, MA, Amburn, E, Baird, A, Larocca
D. Multivalent display of cDNA libraries on filamentous phage. IBC
Antibody Engineering, November 30- December 3, 2003. San Diego, CA.
Burg, M, Ravey, P, Gonzales, MA, Amburn, E, Faix, P, Baird, A, Larocca, D.
Selective Genetics, Inc., San Diego, California: Identification of
internalizing ligands by selection of nucleic acid amplified phage. Vector
Targeting Strategies for Gene Therapy, March 20-23, 2003. Cold Spring
Harbor, NY.
Honors & Awards
STARS (Science, Technology, and Research Scholars) Summer Program 2002.
Yale University, New Haven, CT. (June-August 2002) Intensive summer
program on genomics and bioinformatics funded by a genomics grant from
the USDA.
Ford Salute to Education Scholarship (May 1998) For Outstanding GPA and
community service involvement.
Academic Experience
Relevant Coursework:
Microbiology Biochemistry
Cell & Molecular Biology Forensic Sciences
Immunology Genomics & Bioinformatics
Genetics Analytical Chemistry
Developmental Biology (Overall GPA 3.39)
References
Michael Fitch, Ph.D.
Senior Research Scientist
Tanabe Research Laboratories
4540 Towne Centre Court San Diego, CA 92121
*************@*****.***
Jacqueline Testa, Ph.D.
Associate Professor
PRISM
11107 Roselle Street San Diego, CA 92121
******@*****-**.***
Peg Faix, Ph.D.
Senior Research Scientist
Biomatrica, Inc.
5627 Oberlin Dr., Suite 120
San Diego, CA 92121
*******@*****.***
Alejandro Calderon-Urrea, Ph.D.
Associate Professor
California State University, Fresno
Department of Biology
2555 E. San Ramon Ave. MS SB73
Fresno, CA 93740-8034
*******@*********.***
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