Plant Assistant

Weiqing Zeng, Ph. D.

**** ******** ***., ******, ** 48864

517-***-**** (w), 734-***-**** (m), ********@*****.***

Personal summary

Well-rounded plant molecular biologist with a strong background in protein

biochemistry, cell biology and microbiology. Self-motivated, target-driven

team player with strong commitment to scientific collaboration. Excellent

communication and management skills, and strong verbal, technical writing

and presentation skills. Research and biotechnology oriented teaching

style.

EDUCATION

Ph. D., Cell and Molecular Biology

Michigan State University, East Lansing, MI, 2004.

M.S., Microbiology

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences,

Shanghai, China, 1996.

B.S., Microbiology

Wuhan University, Wuhan, China, 1993.

CErTIFICATE

Business Management and Communication Skills Certificate Program

The Eli Broad Graduate School of Management, the College of Communication

Arts, Michigan State University, East Lansing, Michigan, 2006-2007.

PROFESSIONAL EXPERIENCE

Research Associate

DOE-Plant Research Laboratory, Michigan State University, Feb. 2005 -

present.

Associate

Howard Hughes Medical Institute, Life Science Institute, University of

Michigan, Mar. 2004 - Jan. 2005.

Graduate Research Assistant

DOE-Plant Research Laboratory, Michigan State University, 1997 - 2004.

PROFESSIONAL SERVICES

Grant review:

National Science Foundation.

Journal review:

Plant Physiology; the Plant Journal; Plant & Cell Physiology; Molecular

Plant-Microbe Interaction;

PROFESSIONAL SkillS

Molecular Biology: DNA/RNA isolation and electrophoresis; gene cloning;

southern; northern; RNAi engineering; PCR; RT-PCR; mammalian cell

culturing and transfection;

Biochemistry: Protein isolation; SDS-PAGE/BN-PAGE; western; protein

expression and purification in E. coli, yeast and plants; antibody

design, production and purification; immunoprecipitation; intra-cellular

membrane purification through two-phase partitioning and sucrose

gradient; enzyme activity assays; GC and GC/MS for cell wall

polysaccharide and wax compositions; LC/MS/MS for plant metabolite

analysis;

Cell Biology: light/fluorescent/laser confocal microscopy; SEM; TEM;

protein localization through cellular membrane fractionation, protoplast

transfection, onion epidermal bombardment and agrobacterium transient

expression;

Plant Biology: Plant transformation with Agrobacterium and DNA bombardment;

plant cell culturing; large-scale mutant screening and phenotype

characterization;

Bioinformatics: DNA Star; Vector NTI; Blast; ClustalW; Phylip;

Others: General microbiology skills; general plant genetics skills; general

plant pathology skills;

Teaching experience

Instructor

LBS 290A, Directed Study-Multidisciplinary, Lyman Brigg School, Michigan

State University; Summer Semester, 2007.

Teaching assistant

BOT 416, Experiments in Plant Physiology, Molecular Biology and

Biotechnology Laboratory, Michigan State University; Fall Semester, 1999.

Teaching assistant

BOT 416, Experiments in Plant Physiology, Molecular Biology and

Biotechnology Laboratory, Michigan State University; Fall Semester, 1998.

Publications

Zeng W, He SY (2010) A prominent role of the flagellin receptor FLS2 in

mediating stomatal response to Pseudomonas syringae pv. tomato DC3000 in

Arabidopsis. Plant Physiol. DOI: 10.1104/pp.110.157016.

Zeng W, Melotto M, He SY (2010) Plant stomata: a checkpoint of host

immunity and pathogen virulence. Curr Opin Biotechnol.

DOI:10.1016/j.copbio.2010.05.006.

He SY, Nomura K, Mecey C, Zeng W, Lee YN, Withers J, Imboden L, Uribe F,

Xin X, Yao J, Huang W, Oney M (2010) Pseudomonas syringae pathogenesis in

Arabidopsis. In Antoun H, ed, Biology of Plant-Microbe Interactions, Vol

7, IS-MPMI, St. Paul, Minnesota.

Melotto M, Mecey C, Niu Y, Chung HS, Katsir L, Yao J, Zeng W, Thines B,

Staswick P, Browse J, Howe G, He SY (2008) A critical role of two

positively charged amino acids in the Jas motif of Arabidopsis JAZ

proteins in mediating coronatine- and jasmonoyl isoleucine-dependent

interaction with the COI1 F-box protein. Plant J. 55 (6): 979-988.

He SY, Bray Speth E, Imboden L, Huang W, Lee YN, Melotto M, Mecey C, Nomura

K, Underwood W, Uribe F, Yao J, Zeng W (2008) Suppression of host defense

by Pseudomonas syringae in Arabidopsis. In Lorito M et al., eds, Biology

of Plant-Microbe Interactions, Vol 6, IS-MPMI, St. Paul, Minnesota.

Zeng W, Keegstra K (2008) AtCSLD2 is an integral Golgi membrane protein

with its N-terminus facing the cytosol. Planta, 228 (5): 823-838.

Wen F, Celoy RM, Nguyen T, Zeng W, Keegstra K, Immerzeex P, Pauly M, Hawes

MC (2008) Inducible expression of Pisum sativum Xyloglucan

fucosyltransferase in the pea root cap meristem, and effects of antisense

mRNA expression on root cap cell wall structural integrity. Plant Cell

Rep. 27 (7): 1125-35.

Faik A, Bar-Peled M, DeRocher AE, Zeng W, Perrin RM, Wilkerson C, Raikhel

NV, Keegstra K (2000) Biochemical characterization and molecular cloning

of an alpha-1,2-fucosyltransferase that catalyzes the last step of cell

wall xyloglucan biosynthesis in pea. J Biol. Chem. 275(20): 15082-9.

Faik A, perrin RM, Sarria R, Zeng W, Wilkerson C, Raikhel NV, Keegstra K

(2000) Plant cell wall biosynthesis: Identification and characterization

of fucosyltransferase and putative galactosyltransferases involved in

xyloglucan biosynthesis. Glycoconj. J 17 (1-2): 78.

Baikhel NV, Faik A, Perrin RM, Sarria R, Zeng W, Wilkerson C, Keegstra K

(2000) Xyloglucan biosynthesis. Glycoconj. J 17 (1-2): 75.

Perrin RM, DeRocher AE, Bar-Peled M, Zeng W, Norambuena L, Orellana A,

Raikhel NV, Keegstra K (1999) Xyloglucan fucosyltransferase, an enzyme

involved in plant cell wall biosynthesis. Science. 284 (5422): 1976-9

Zeng W and Song H (1997) Cloning of an unlinked exo site from EPS synthesis

deficient mutants of Rhizobium astragali strain 107. Acta

Phytophysiologica Sinica. 23(4): 331-336.

manuscripts submitted

Gao X, Koo AJK, Zeng W, Howe GA, Staswick PE, He SY, Jones AD (2010)

Jasmonate-centric profiling of phytohormone levels in plant responses to

stress using ultra performance liquid chromatography-tandem mass

spectrometry. Anal Chem.

manuscripts in preparation

Zeng W, Gao X, Jones D, He SY (2010) A genetic screen for Arabidopsis

mutants defective in resistance to coronatine-deficient Pseudomonas

syringae pv. tomato DC3000.

Zeng W, Krassovskaya I, Li Y, Wang Q, Wang Y, He SY (2010) A

thioesterase/hydrolase, SCD (Susceptible to Coronatine-Deficient Pst

DC3118) 2, is involved in the aldehyde-to-alkane conversion during

Arabidopsis epicuticular wax biosynthesis.

INvited presentations

Arabidopsis stomatal defense against Pseudomonas syringae pv. tomato

DC3000. 2010, Department of Biology, Eastern Michigan University

Identification and characterization of the Arabidopsis mutant scd2

(susceptible to coronatine-deficient Pseudomonas syringae pv. tomato

strain DC3118). 2007, ASPB Midwest Section Annual Meeting

Patent

Weiqing Zeng and Sheng Yang He. SCD2 encodes an enzyme involved in the

biosynthesis of alkanes from aldehydes in Arabidopsis epicuticular wax

(No. 12/695,605), 2009. Pending.

PROFESSIONAL MEMBERSHIPs

American Society of Plant Biologists

The Society for Glycobiology

International Society for Molecular Plant-Microbe Interactions

Activities

Chair, Steering Committee, MSU Postdoctoral Association, Michigan State

University, 2009-2010

Vice Chair, Steering Committee, MSU Postdoctoral Association, Michigan

State University, 2008-2009

Teaching Assistant facilitator for international TA's orientation, domestic

TA's orientation, Teaching Assistant Program, Michigan State University.

Volunteer facilitator for international student orientation, Office of

International Student and Scholars, Michigan State University.

Served in various departmental committees at DOE-Plant Research Laboratory

of Michigan State University.

Supervised undergraduate students and rotation graduate students.

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