Assistant Engineering

Harsha Krovi

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*** ****** ****** **** ***********, NC 27560

978-***-**** *****@********.***

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EDUCATION

UNIVERSITY OF VIRGINIA Charlottesville, VA. 2006-2010.

B.S. in Biomedical Engineering and Minor in Chemical Engineering

Overall GPA: 3.6

PERSONAL INFORMATION

. U.S. Citizen

SKILLS

. Extensive experience with cell culturing and protein adsorption

techniques

. Conducted numerous ELISAs, PCRs, Western Blots, cell cloning

experiments and gel electrophoresis experiments

. Used confocal and fluorescent microscope for immunofluorescence

visualization

. Tutored students in C/C++ and Java

. Made extensive use of MATLAB and MathCAD in class assignments and

projects

. Expertise with Microsoft Office products, especially Microsoft Excel

and Microsoft Access

. Working knowledge of HTML, XML, Flash 9, ActionScript 3.0, SAS, SQL

WORK EXPERIENCE

University Of Virginia, Center for Applied Biomechanics Charlottesville, VA

September 2009-May 2010

Lab Assistant

. Used ferrets as a comparative brain model for humans to determine

extent of traumatic brain injury due to blast

. Employed visual and auditory evoked potentials (EPs) to evaluate

severity of blast-induced injury in ferrets

. Analyzed a significant amount of data using MATLAB to obtain

statistical parameters

. Conducted time-series and Fourier analysis to create plots which

depicted extent of injury with respect to time and frequency,

respectively

. Conducted serum analysis and ELISA assays to identify brain-specific

biomarkers which escaped into the bloodstream after the blast-induced

injury

. Maintained a regular journal detailing experimental procedures

. Met regularly with the primary investigator to discuss progress of

project

Lawrence Biomedical Engineering Lab January 2009-May 2010

Lab Assistant

. Determined proteins involved in lymphocyte-endothelial cell

interactions using various protein identification assays

. Employed an in-lab patented fluidics device to determine on- and off-

bond rates between flowing lymphocytes and adhered endothelial cells

. Implemented parallel flow-chamber assay repeatedly to identify the

extent of rolling of lymphocytes on endothelial cells

. Conducted flow cytometry experiments to measure concentration of

lymphocytes

. Performed protein assays to quantify concentration of protein in

samples

. Cultured numerous cell-lines regularly to ensure that the cells

remained robust and expressed appropriate proteins when required

Horwitz Cell Biology Lab September 2007-May 2008

Lab Assistant

. Conducted bacterial transformations using both Midi and Maxi-preps

. Used a centrifuge to spin down bacteria and obtain plasmid

. Performed mass spectrometry to determine ratio of RNA to DNA in

centrifuged sample

. Autoclaved and prepared Luria broths

Intro to Computer Science (Java) January 2007-May 2007

Teaching Assistant

. Assisted students with homework assignments and projects

. Graded assignments, projects, and exams

Advanced Liquid Logic, Morrisville, NC June 2008-August 2008

Software Programmer in Python

. Developed and programmed assays in Python to automate them in a

microfluidics device

. Coded fluidics assays for engineers who implemented them using the

device

. Successfully utilized the Python object-oriented programming (OOP)

environment to create new classes and expand upon the functionality of

existing classes

. Updated and debugged antiquated code for increased efficiency

Charlottesville Running Company, Charlottesville, VA September 2007-May

2009

Cashier and Gait Analyst

. Analyzed gait of individuals looking to purchase running shoes and

recommended shoes that best complemented their gait

. Managed sales and returns of store goods

LEADERSHIP EXPERIENCE

Biomedical Engineering Society (BMES)Charlottesville, VA December 2009-May

2010

Social Chair

. Organized social gatherings for BMES members at UVa

SOCA, Madison House August 2007 - May 2008

Volunteer Soccer Coach

. Coached a U-8 soccer team

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