Data Years Experience

Tingting Yue

*** ******* ***, ****, ** *****

*****.***@*****.*** (Cell) 919-***-****

Objective

A motivated and detail-orientated scientist with 4 years experience of

analytics in clinical research, plus a multidisciplinary training and

experience in science, engineering, bioinformatics, epidemiology and

statistics, is seeking a full-time analysis and research position in

life-science industries in North Carolina.

SUMMAry OF qualifications

Work Competence

Four years experience in bioinformatics and statistical analysis for

Phase I & II biomarker study. Expertise in study design of clinical

screening assays, bioinformatics data-mining of "omics" study, and

statistical data analysis for high-throughput study. In-depth training

in next-gen sequencing analysis. Certified SAS programmer. Proficiency

in multiple bioinformatics and statistical tools.

Communication Skills

Demonstrated success in presenting and documenting research results for

scientific publications in "omics" journal and epidemiology meeting.

Successful experience in communicating bioinformatics and statistical

data processing to non-statisticians. Excellent interpersonal skills

developed from leading research and collaborating with researchers in

industry and academics. Experience in student training and knowledge

transfer.

Additional expertise and potential

Four years hands-on experience in conducting data collection of clinical

research plus 1 year experience in regenerative medicine research. A

professional sense of data analysis developed from the multidisciplinary

research experience. Quick learner of new data analysis methods and new

domain knowledge.

Education

10/2010 Ph.D. Cell and Molecular Biology, Michigan State University,

East Lansing, United States.

12/2005 Master of Research (M.Res.) Regenerative Medicine, Center of

Regenerative Medicine, University of Bath, Bath, United Kingdom.

06/2003 B.S. Biotechnology, Huazhong University of Science and

Technology, Wuhan, China

Certification SAS base programming certificate for SAS 9

Experience

05/2006 - Present, Van Andel Research Institute, Grand Rapids, MI, Graduate

research assistant. Lead scientist of the Phase I & II biomarker study in

Laboratory of Cancer Immunodiagnostics.

Bioinformatics analysis to develop biomarker panel enhancing cancer

diagnosis

-Developed a patentable biomarker panel to improve patient

stratification and reduce the healthcare cost for pancreatic cancer

diagnosis.

-Developed and optimized a glycoproteomic solution for diagnostic

biomarker development.

-Developed a standard strategy which is continuously used by the lab to

select biomarker panel.

-Characterized the biological insights of the molecular epidemiology of

the biomarkers.

-Designed and conducted the biomarker screening of 400 candidate markers

from 5 independent sample sets including 450 clinical samples.

Database development for Consortium for Functional Glycomics (CFG)

glycan microarray

-Implemented the motif-segregation algorithm to convert 17,244 raw

microarray data values into a widely-used public database providing

information of lectin binding specificities.

Multiplex microarray method testing and evaluation for Gentel

Biosciences, Inc.

-Tested and evaluated the method at a high-throughput sample processing

platform.

-Improved the cost-effectiveness of this protocol which has been

commercialized successfully.

Verbal and written presentation of research

-Published more than 5 peer-reviewed articles about high-throughput

clinical study and database development.

-Presented the data analysis at 9 international conferences in different

fields including cancer research, proteomics, clinical research,

glycomics, and epidemiology.

-Assisted technology transfer by documenting invention disclosures for

the patent application of a diagnostic biomarker panel.

-Provided technical report to Gentel Biosciences, Inc. for the

evaluation of the multiplex assay.

-Secured additional funding by documenting successful research results

in a grant application.

-Provided description of published results for press release targeting

non-scientific readers.

-Effectively communicated with collaborators including physicians,

statisticians and biologists.

10/2004-03/2005 Center of Regenerative Medicine, University of Bath, U.K.

Research student

Dept. of Chemical Engineering In vitro simulation of PLGA scaffold

degradation for bioreactor design in bone tissue engineering.

Dept. of Biology and Biochemistry Characterized expression pattern of

gene Id2 in the zebrafish pigmentation model to study the

differentiation of neural crest stem cells.

Teamwork and Leadership

05/2006 -06/2010 Collaborated with Early Diagnosis Research Network

(EDRN) for biomarker development (Collaborators including Evanston

Northwestern Healthcare, U of Pittsburgh, U of Michigan, Fred Hutchinson

Cancer Research Center, Michigan Proteome Consortium).

01/2010-06/2010 Led data collection and bioinformatics analysis of a

double-blinded clinical profiling study collaborating with U of

Pittsburgh and Fred Hutchinson Cancer Research Center.

06/2008-06/2010 Technical training and research mentoring of 2 lab

technicians and 3 undergraduate student interns.

Teaching experience

08/2006-12/2006 Teaching assistant, Dept. of Biochemistry and Molecular

Biology, MSU, Course title: "Introduction to Biochemistry", class size:

96 undergraduate students

SUMMAry OF skills

Analytical and Computer Skills

Statistical: Multivariate analysis, Receiver operating characteristic

(ROC) analysis, Survival analysis, Statistical regression, Bayesian

methods, Power calculation, Categorical analysis, Non parametric

analysis, Analysis of variance (ANOVA), Machine learning, Pattern

recognition, Hypothesis testing, cluster, Multiple comparisons

Bioinformatics: Microarray and mass spectrometry data-mining, Study

design for high-throughput screening, Data mining and data management

for molecular biomarker study, Biomarker panel selection, Genome-wide

association study (GWAS) analysis by GenABEL, Patient stratification for

drug development, CGH expression data analysis

Software/programming: SAS (certified programmer), R, MATLAB, Origin,

Cluster, Perl, Atlas.ti

Research Skills

General: DNA/RNA/protein isolation, Molecular cloning, Gene knockdown,

RT-PCR, Real-time PCR (Q-PCR), PCR, In-situ hybridization, Western Blot,

Immunoprecipitation, Protein purification, Cell culture, Cell-based

assays

Proteomics: Protein microarray assays, Glycoproteomics assays, ELISA

Equipment: Automated liquid handler, High-performance liquid

chromatography (HPLC), Fast protein liquid chromatography (FPLC), Atomic

force microscopy, Microarray printer

Awards

Graduate Student Fellowship (Van Andel Research Institute, 2005-2006)

Student Travel Award (Society of Glycobiology annual meeting, 2008 and

2009)

Student Travel Award (US HUPO annual meeting, 2010)

Scholar-In-Training Award (American Assc. of Cancer Research (AACR)

annual meeting 2010)

Scholar-In-Training Award (AACR Molecular Epidemiology annual meeting,

2010)

Dissertation Completion Fellowship (Michigan State University, 2010)

Publications

1. T. Yue, I.J.Goldstein, M.A.Hollingsworth, K.Kaul, R.E.Brand, B.B.Haab

(2009). The prevalence and nature of glycan alterations on specific

proteins in pancreatic cancer patients revealed using antibody-lectin

sandwich arrays. Mol Cell Proteomics. 8,1697-1707

2. T. Yue, B.B.Haab (2009). Microarrays in glycoproteomics research. Clin

Lab Med. 29, 15-29.

3. A.Porter, T. Yue (co-first author), L.Heeringa, S.Day, E.Suh, B.B.Haab

(2010). A motif-based analysis of glycan array data to determine the

specificties of glycan-binding proteins. Glycobiology. 20(3):369-80

4. B.B.Haab and T. Yue (2010). High-throughput studies of protein

glycoforms using antibody-lectin sandwich assays. Methods Mol.Biol.

(In press)

5. B.B.Haab, A.Porter, T. Yue, L.Li, J. Scheiman, M.A.Anderson, D.Barnes,

C.M.Schmidt, Z.Feng, D.M.Simeone (2010). Glycosylation variants of

mucins and CEACAMs as candidate biomarkers for the diagnosis of

pancreatic cystic neoplasms. Annals of Surgery. 251(5):937-45.

6. T. Yue, R.E.Brand, M.A.Anderson, D.M.Simeone, B.B.Haab. Enhanced

sensitivity of pancreatic cancer detection by measuring the CA19-9

antigen on selected protein carriers. (In preparation for submission

to JNCI or Cancer Res)

7. T. Yue, R.E.Brand, M.Hurley, P.Andrews, B.B. Haab. Identification of

novel CA19-9 carrier revealed the need to fine-tune CA19-9 for

pancreatic cancer detection. (In preparation)

Conference presentations

1. T. Yue, I.J.Goldstein, M.A.Hollingsworth, R.E.Brand, B.B.Haab (2007).

Glycan variation on serum mucins in pancreatic cancer. Annual meeting

of the Society of Glycobiology. (Poster)

2. T. Yue, I.J.Goldstein, M.A.Hollingsworth, R.E.Brand, B.B.Haab (2008).

Glycan alterations on serum mucins in pancreatic cancer. Annual

meeting of American Association of Cancer Research (AACR). (Poster)

3. A.Porter, T. Yue, L.Li, J.Scheiman, M.A. Anderson, D.Barnes,

C.M.Schmidt, Z.Feng, D.M. Simeone, B.B.Haab (2008). Glycosylation

Variants of Mucins and CEACAMs As Candidate Biomarkers for the

Diagnosis of Pancreatic Cystic Neoplasms. American Pancreatic

Association annual meeting. (Oral presentation)

4. T. Yue, I.J.Goldstein, M.A.Hollingsworth, K.Kaul, R.E.Brand, B.B. Haab

(2008). The prevalence and nature of glycan alterations on specific

proteins in pancreatic cancer patients revealed by antibody-glycan

sandwich arrays. Annual meeting of the Society of Glycobiology.

(Poster)

5. T. Yue, I.J.Goldstein, M.A.Hollingsworth, K.Kaul, R.E.Brand, B.B. Haab

(2009). Varation in carrier proteins of the CA19-9 antigen in patients

with benign and malignant pancreatic disease. Annual meeting of AACR.

(Poster)

6. A.Porter, L.Heeringa, T. Yue(co-first author), G.Michailidis, B.B.Haab

(2009). A motif-based analysis of glycan array data to define the

specificities of glycan-binding proteins. Annual meeting of the

Society of Glycobiology. (Poster)

7. T. Yue, L.Li, M.A.Anderson, D.E.Brenne, D.M.Simeone, Z.Feng,

R.E.Brand, B.B.Haab (2010). Candidate Biomarkers for Pancreatic Cancer

Evaluated Using Antibody-Lectin Sandwich Arrays in Multiple Sample

Sets. Annual meeting of US HUPO. (Oral presentation)

8. T. Yue, M.C.Hurley, R.E.Brand, P.C. Andrews, B.B. Haab (2010).

Pancreatic disease-specific carriers of the CA 19-9 antigen identified

by antibody microarrays and mass spectrometry. Annual meeting of AACR.

(Poster)

9. T. Yue, L.Li, M.A.Anderson, D.M.Simeone, Z.Feng, R.E.Brand, B.B.Haab

(2010). Candidate biomarkers for pancreatic cancer evaluated using

antibody-lectin sandwich arrays in multiple serum sample sets. AACR

special meeting, "The Future of Molecular Epidemiology". (Poster)

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