Quality Assurance Manager

D eanna Collia

*** ***** ** *.*. ~ Palm Bay, Florida 32905

H o me ( 3 2 1 ) 6 7 6 . 9 9 7 2 *******@***.*** C el l ( 7 7 2 ) 9 1 3. 5 5 0 1

Experienced research associate seeking a challenging position in the biomedical science field.

Sanford Burnham Medical Research Institute

Research Associate 01/2009 – present

• Designed and conducted in vivo experiments to elucidate the role of PGC1 in brown adipose tissue in mouse and its

relative contribution to obesity and diabetes

• Harvested and cultured primary embryonic fibroblasts from mouse

• Developed and implemented an in depth Excel database for maintaining and tracking animals in a colony exceeding 600

cages

• Successfully cloned 5 new plasmids for the laboratory using the Topo cloning system

• Performed a head to head comparison of real time PCR reagents which resulted in $1000.00/month overall cost savings in

the lab

• Developed and optimized multiple end point PCR protocols for genotyping various new transgenic models

• Supervised, trained, and directed scientific staff on laboratory and vivarium policies and procedures

• Generated supplemental figures in Impaired contractile function and calcium handling in hearts of cardiac specific

calcineurin b1 deficient mice, Am J Physiol Heart Circ Physiol, 2009.

• Presented experimental data to scientific director and laboratory manager in semi monthly progress meetings

• Facilitated and organized all daily lab operations, including purchasing and receiving

• Maintained detailed inventory logs of laboratory reagents, cells, and samples

Midwest Research Institute

Intern/Technician 06/2007 – 10/2007

• Ran assays for the development of novel products for the identification and amplification of compromised human DNA

using translesion polymerases

• Analyzed scientific data and maintained detailed records for patenting purposes

• Generated scientific data for the development of an assay to further Human Forensic Science through targeted genome

amplification

• Redesigned several primers for targeted genome amplification

Skills

Molecular Biology: End Point PCR, rtPCR, Sequencing, RNA/DNA extractions from tissue, cDNA synthesis, Agarose Gels, SNP

Analysis, Cloning, Genotyping, primer design

Protein Chemistry: Bradford protein determination assays, Western blotting, SDS PAGE, protein expression & purification

Microscopy: Proficient in the use of various microscopes.

Tissue Culture: sterile technique for cell culture, cell passage and propagation, basic mammalian cell culture

Small Animal Management: Animal handling including: restraint, IP and IV injections, oral gavage, tagging and tailing, and overall

wellness, Dissection and harvesting of multiple tissues including: heart, liver, lung, spleen, brain, thymus, kidney, embryos, brown and

white adipose tissues, and skeletal muscle. In vivo experiments including: glucose tolerance testing, insulin tolerance testing, weight

studies, body analysis using NMR, cold exposure, exercise studies, and high fat diet studies.

Equipment: BioRad ChemiDoc and Alpha Innotech analyzer, ABI 3730, ABI 3100, Stratagene Mx3000P and iCycler QPCR, Laser

Capture Dissection Microscope, Bruker the MiniSpec NMR

Software: Microsoft Office Suite (Excel, PowerPoint, Word, Publisher, Access, Outlook, OneNote), FileMaker Pro, SciQuest, Primer3,

BLAST, Vector NTI, GeneMapper

Specialized training: Detailed Record keeping, Decontamination and Titer flow Training, Quality assurance training, Radioactive

Materials, BSL 2

Masters of Science (currently pursuing); Advanced Biotechnology – John’s Hopkins University, Baltimore, Maryland

Bachelors of Science; Molecular and Microbiology – August 2007 University of Central Florida, Orlando, Florida

AA Degree– May 2005 – Brevard Community College, Cocoa, Florida

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