Rui-Hua( Carrie) Peng ( Curriculum

Vitae)

Address:

UF& Shands Medical Laboratories at Rocky Point

Hematopathology, Flowcytometry Laboratory

**** ** ** *****, **** 1108

Gainesville, Florida 32608

Phone:352-***-****(home)

352-***-****(cell)

Email: abkrqf@r.postjobfree.com

Personal data

NAME Ruihua Peng SEX Female

MARITAL STATUS Married DOB

JOB TITLE Medical Health Excellent

Technologist

II: Education and training

TIME PERIOD EDUCATION INSTITUTE DIPLOMA AWARDED

Medical bachelor degree

Sep.,1983 Weifang Medical College

to July 1988

October 31, BD Biosciences Certificate for Flow

2002 Cytometry training and

workshop.

Sep, American Medical Technologist MT certification

2007-present

Sep, Florida State Health Department Florida State MT License in

2007-present the specialties of

Hematology, Chemistry,

Serology, Microbiology and

Immunology.

Clinical & Research or Professional Experience:

June, 2008 to present: Medical Technologist Shands hospital at University

of Florida, Hematopathology Flow Cytometry Lab

Performing daily instrument QC

o Performing Routine instrument setup and shutdown.

o Performing daily CST performance check on

FACSCantoII and FACS Aria.

o Optimization FACScantoII and FACSAria compensation

using BD FACSComp Beads and using patient's PB cells

to check this compensation setting.

o Using CaliBRITE Beads to check the PMT voltage, Time

delay and compensation on FACSCalibur and FACSscan

o Runing CVCK Align Flow beads for CV check.

Performing Quality control of specimen and

unlysed RBC: Viability check using PI( propidium iodide) and

Cytospin preparation.

Performing cell positive cellular controls :

. CD-Chex Plus for CD4+ cells (two levels)

. CD-Chex for CD34+ cells ( two levels)

Flow Cytometry analysis:

o Performing immune phenotyping tests on peripheral

blood

o Preparing cell suspension from Bone marrow,

Peripheral blood, Tissue, Body fluid, and Fine needle

aspirates in leukemia/lymphoma, multiple myeloma and

PNH patients for flow cytometry.

o Grossing Bone marrow biopsies, clots and solid

tissue.

o Performing cell surface antigen staining and

intracytoplasmic staining (Kappa and Lamda light

chains, BCL2, ZAP70, MPO staining and nuclear TdT

staining).

o Performing RBC, platelet and leukocyte staining on

PNH patient samples.

o Performing T cell receptor (TCR) V-beta analysis by

flow cytometry.

o Per forming DNA content analysis (cycleTEST or Drag5

staining).

o Acquiring samples on 4Color FACsCaliber, 3Color

FACscan, 6Color FACsAria and FACsCanto II cytometers.

Flow Cytometry data analysis: Performing Flow data analysis

using Cell Quest, FCS express, FACsDiva Software and

ModFit LT software for DNA analysis.

Reporting Flow data for Immune Phenotyping

tests in our Mysis database.

Performing reagent QC: Antibody QC and Titration,

Tray products QC, Blood bank products QC and PBS Buffer, CPM

medium QC etc.

Performing CAP offered proficiency tests and

parallel study (leukemia/lymphoma panel, immunephenotyping

tests, CD34, DNA, PNH )

Tracking performance over time: CVs, PMT voltage

required to hit target values, compensation, laser status,

threshold and Levey-Jennings plots.

Performing manual staining: Wright-Giemsa staining and MPO

(myeloperoxidase), NSE (non-specific esterase), IRON staining

and Immunochemistry staining.

Managing clinical sample receipt, documentation and specimen

accessioning in the our Powerpath database.

Performing problem-solving and trouble-shootting.

Communicating with clinical sites on sample scheduling,

labeling, acceptance/rejection, transportation, storage and the

data reporting.

October, 2003 to June, 2008: Biological Scientist, clinical trial

coordinator and lab manager: Department of Pathology, Immunology

and Laboratory Medicine, University of Florida.

Participating clinical trials: I was participating in

several clinical trial studies. The techniques used in those trials

are as follows:

a. Processing human blood samples to obtain PBMCs.

b. ELISA for cytokines detection in serum samples and whole blood

culture supernatant

c. Performinf Flow cytometry analyais on FACs ca to characterize

immunophenotypes of B cells, T cells, Monocytes, Dendritic cells

and NK T cells.

d. Performing T cell sorts on cytometry analyzer.

e. MLR(mix leukocyte reaction) for T cell proliferation assay.

f. Luminex for multiple cytokine detection and chemokine assay.

g. ELISpot technique for rare cytokine-producing cell assay.

h. T cell and Dendritic cell purification using MACSorting or

FACSorting or StemCell Sep (negative selection for CD4 T cells)

techniques.

i. DNA and RNA extraction and Real-time PCR.

j. HLA typing for Screening HLA-DR4+ type 1 diabetes patients.

k. QC/QC data report

Flow cytometry analysis:

a. Performing instruments daily QC and machine set-up

b. Preparing flow samples including surface staining and

intracellular staining.

c. Running FACS samples on FACSCaliber, FACsScan, LSRII.

d. Analyzing flow cytometry data using FCS express, FACsDiva and

Cell Quest software.

e. Handling problem-solving and trouble-shooting.

Coordinator duties:

Coordinating or Communicating with our clinical sites or DRL (

diagnostic reference lab) and outside diabetes center on sample

scheduling, labeling, shipping/ receiving acceptance/rejection,

transportation, storage and the QC/QA data reporting.

Lab manager other duties:

Organizing the labs including all required

documents for research and lab safety, lab equipment maintenance,

calibration, quality control and quality assurance. Supervising

the rotating graduate students and volunteering undergraduate

students. Performing lab supply orders and tracking inventory of

reagents and chemicals in the lab.

Animal experimental skills: Tail vein injection,

intraperitoneal injection, foot pad injection. Retro-orbital

bleeding, T cell preparation from mouse spleen, lymph nodes, as

well as pancreas and bone marrow cell preparation.

Other techniques: Western blotting,

immunoprecipitation, preparing samples for DNA microarray.

Immunohistochemistry, and fluorescent microscopy imaging.

Computer skills: word, excel, powerpoint, access,

prism, Cellquest and FCS express for flow cytometry data

analysis. ELISpot bioreader software, Luminex cytokine assay

(beadview software).

July, 2001-September, 2003: Postdoctoral research associate. Dept of

Pathology, Immunology, and Laboratory Medicine, University of

Florida.

Research project: Characterization of

peripheral blood dendritic cell subsets in type 1 diabetes and

healthy control subjects.

Major techniques used: Flow cytometry

for evaluating dendritic cell subset frequency, absolute number

as well as immunophenotypes in type I diabetes patients.

Dendritric cell isolation from PBMCs using magnetic cell sorting

technique. ELISA, Luminex, cell culture, DNA array, Real-time

PCR, Gene array using gene chips, western blotting.

Other duties: Preparation of research data

for academic conference, manuscripts, grants application.

Computer skills: word, excel, powerpoint,

access, prizm. FCS express for flow cytometry data analysis.

ELISpot bioreader program, Luminex cytokine assay program.

October, 2000- June, 2001: Research Assistant. Dept of

Pathology, Immunology, and Laboratory Medicine, University of

Florida.

Projects: Analysis of PGS2 Expression in human

monocytes and mouse bone marrow myeloid cells.

Techniques: Flow cytometry, cell culture,

cell isolation from spleen and bone marrow, handling animal

model, Real time PCR, Gene assay, Western blot,

immunohistochemistry.

Computer techniques: word, excel, access,

powerpoint, gel image, FCS express for analysis of flow

cytometry data.

June, 2000-September, 2000 Laboratory technician. Cancer Center,

College of Medicine, University of Florida. Techniques: DNA

extraction from bacteria and cells, plasmid transduction,

western bloting, and RT-PCR.

August, 1988-July, 1999,June

Clinician in a clinical lab. Weifang

hospital. China.

Publication

1. Xia CQ, Qiu Y, Peng RH and Lo-Dauer J, Clare-Salzler, MJ. Infusion of

UVB-treated splenic stromal cells induces suppression of beta cell

antigen-specific T cell responses in NOD mice. J Autoimmun. 2008

Jun;30(4):283-92.

2. Xia CQ, Qiu Y, Peng R, Lo-Dauer J, Clare-Salzler MJ. Apoptotic non-

beta cells suppress beta cell antigen-reactive T cells and induce beta

cell antigen-specific regulatory T cells. Ann N Y Acad Sci. 2008

Dec;1150:167-70.

3. Xia CQ, Peng Rui-hua. Qiu, Yushi, Annamalai M, Gordon D, and Clare-

Salzler. Transfusion of apoptotic beta cells induces immune tolerance

to beta cell antigens and prevents type 1 diabetes in NOD mice.

Diabetes. 2007, 56:2116-2123.

4. Xia CQ, Peng Rui-Hua, Annamalai M, Clare-Salzler MJ. Dendritic cells

post-maturation are reprogrammed with heightened IFN-gamma and IL-10.

Biochem Biophys Res Commun. 2007 Jan 26;352(4):960-5

5. Peng Rui-Hua, Paek E, Xia CQ, Tennyson N, Clare-Salzler MJ. Heightened

interferon-alpha/beta response causes myeloid cell dysfunction and

promotes T1D pathogenesis in NOD mice. Ann N Y Acad Sci. 2006

Oct;1079:99-102.

6. Lo J, Peng Rui-Hua, Barker T, Xia CQ, Clare-Salzler MJ. Peptide-pulsed

immature dendritic cells reduce response to beta cell target antigens

and protect NOD recipients from type I diabetes. Ann N Y Acad Sci.

2006 Oct;1079:153-6.

7. Xia CQ, Peng Rui-Hua, Beato F, Clare-Salzler M. Dexamethasone induces

IL-10-producing monocyte-derived dendritic cells with durable

immaturity. Scand J Immunol. 2005 Jul;62(1):45-54.

8. Womer KL, Peng Rui-Hua, Patton PR, Kaleem A, Bucci M, Murawski MR,

Srinivas T.R., Meier-Kriesche HU, Kaplan B, Clare-Salzler MJ, The

Effects of Renal Transplantation on peripheral blood dentritic cell.

Transplant Proc. 2005 Jan-Feb;37(1):3-6.

9. Womer KL, Peng Rui-Hua, Patton PR, Kaleem A, Bucci M, Murawski MR,

Srinivas T.R., Meier-Kriesche HU, Kaplan B, Clare-Salzler MJ,Precursor dentical cell subset ratio in peripheral blood of rental

transplant recipient correlates with level of immunosuppresion. Clin

Transplation 2005 DOI: 10.1111/J.1399-0012.2005.00405.x

10. Peng Rui-Hua, K. BATHJAT, Y.LI, AND M.J.CLARE-SALZLER. Defective

Maturation of myeloid Dendritic Cell in NOD Nice Is Controled by

IDD10/17/18. Ann N Y Acad Sci. 2003 Nov;1005:184-6

11. Peng Rui-Hua, Y.LI, K. BREZNER, SALLY LITHERLAND and M.J.CLARE-

SALZLEER. Abnormal peripheral blood dendritic cell populations in

type 1diabetes. Ann N Y Acad Sci. 2003 Nov;1005:222-5.

12. Litherland, SA, J-X She, D Scbatz, K Fuller, AD Hutson, Rui-Hua Peng,

et al. Aberrant Monocyte Prostaglandin Synthase2(PGS2) Expression in

Type 1 Diabetes Before & After Disease. Pediaric Diabetes, 2003, 4: 10-

18.

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