Training Sales

SHYAMALI ROY

*** ******** ******

Milpitas, CA **035.

Ph# 408-***-**** (H), 408-***-**** (C) Email: *******@*******.***

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OBJECTIVE

To gain employment as a scientific professional where my talents, skills,

and knowledge can all be used effectively.

RESEARCH & PROFESSIONAL EXPERIENCE

Life Science Technician II, Stanford University, CA.

April, 2007 - Present

Job Responsibilities:

. Studying the molecular trafficking mechanism of Theilers virus, a

neurotropic picornavirus, from axon to myelin by using in vivo mouse

model.

. Working as regulatory consultant for Invitek Inc.

. Human/Mouse Embryonic stem cell culture and cardiomyocyte

differentiation.

Associate Scientist, Bexel Pharmaceuticals, Union City, CA.

April, 2005 - October, 2005

Job Responsibilities: Conduct in vitro (perform cellular assays to

determine compound pharmacology/screening) and in vivo (murine model)

preclinical studies (drug administration, efficacy studies, necropsy and

blood serum analysis in mice) evaluating lead compounds for the treatment

of obesity. Make presentations at departmental meetings and assist in the

preparation of product documentation/SOP.

Training Specialist, Cellomics, Inc., Pittsburgh, PA.

November, 2001- January, 2002

Job Responsibilities: Design, perform and optimize cell-

based assays on Cellomics HCS system and interpret experiments; conduct

training sessions for customers and internal staff. Product testing &

validation. Work closely with the Marketing/sales groups. Provide follow-up

technical support. Review and recommend revisions in training methods to

facilitate the production of continually improved and updated training

programs and materials. Assist in developing training support materials.

Actively participate in Marketing product exhibits & trade shows.

Field Application Scientist, Cellomics, Inc., Pittsburgh, PA.

April, 2001 - October, 2001

Job Responsibilities: Design, perform and optimize cell-

based assays on Cellomics HCS system and interpret experiments, pre-sales

technical presentations, instrument training, designing and conducting

feasibility studies and experiments to integrate reagents, hardware and

software, attending scientific conferences, managing in-house

demonstrations of company's product technology, troubleshooting and

calibration of the instrument, and generating data suitable for inclusion

in technical marketing materials.

Post-doctoral Research Associate

March, 1999 - April, 2001

Vanderbilt University School of Medicine, Nashville, TN.

Project: "Role of Focal Adhesion Kinase (FAK) in neoplastic transformation

induced by v-Src".

Post-doctoral Research Associate

May, 1998 - February, 1999

University of Tennessee, Memphis, TN.

Project: "Identifying the role of protein tyrosine phosphatase 1B

(PTP1B) in rat vascular smooth muscle cell migration and proliferation".

ACQUIRED RESEARCH TECHNIQUES

. High throughput (high-content) cell-based assays using image analysis,

Calcium flux assays.

. Instrumentation: Array Scan High Content Screening System, Dexa

Scanner for in vivo imaging, Microtome, HPLC, FacScan.

. Cell & Tissue culture techniques: transient/stable transfection with

DNA and RNA, in vivo transfection with RNA, clonal selection, Human

cell lines (PBMC), primary rat vascular smooth muscle cell, primary

mouse bone marrow derived macrophages, human/mouse embryonic stem cell

culture.

. Bacteriology: Bacterial pathogens (E.coli, S.typhi,

S.dysenteriae,V.cholerae) and microbial aseptic techniques,

adherence/virulence/haemmaglutination assay.

. In Vivo Animal Model: Murine model for obesity study (Diet- induced

and ob/ob mice), murine model for neuronal pathogenesis by Theilers

virus (retinal infection, perfusion, isolation of spinal cord, whole

mount retina and optic nerve), Rabbit Ileal loop model.

. Laboratory technique for analysis of proteins: Native and SDS PAGE,

Electroporation.

. Enzymology: Enzyme assay (kinase), protein assay, In-gel phosphatase

assay.

. Spectrometry and scintillation system: Fluorescence

spectrometry/microscopy, UV- visible spectrometry, liquid

scintillation counter and Autoradiography.

. Immunological techniques: ELISA, Western blotting,

immunohistochemistry eg. Immunofluoroscence, Immunodiffusion, and

Immunoprecipitation.

. Molecular biology: Plasmid DNA isolation, purification, restriction

enzyme digestion, PCR, primer design, cloning, bacterial recombinant

protein expression and purification, in vitro RNA transcription,

transformation, conjugation.

. Computer Skill: GraphPad Prism, SoftmaxPro, MS Word, MS Excel, MS

Power Point, Flowjo, Adobe Photoshop, Dreamweaver/HTML (beginner),Unix

(beginner), SQL (beginner).

. Some Bioinformatics tools and databases.

PUBLICATIONS

1. Jose Antonio Gomez, Shyamali Roy, Jean-Fran ois Bureau, Smita Gopinath,

Denise M. Monack, Karla Kirkegaard and Michel Brahic. "Effect of A murine

Tmevp3 locus on infection by Salmonella typhimurium". Manuscript under

preparation.

2. Eric Freundt, Shyamali Roy, Michel Brahic, Ronald Melki and Karla

Kirkegaard. Axonal transport and cell-to-cell transmission of ?-synuclein

aggregates. Manuscript under preparation.

3. Eric Freundt, Shyamali Roy, Michel Brahic and Karla Kirkegaard. The role

of proteolipoprotein (PLP) in axon-to-myelin spread of Theiler's murine

encephalomyelitis virus.Manuscript under preparation.

4. Roy, S, Ruest, P. J., and Hanks, S. K. "FAK regulates tyrosine

phosphorylation of CAS, Paxillin, and PYK2 in cells expressing v-Src, but

is not a critical determinant of v-Src transformation". J Cellular Biochem

(2002) 84: 377-388.

5. Ruest, P.J., Roy, S., Shi, E., Mernaugh, R.L., and Hanks, S.K.

"Phosphospecific antibodies reveal focal adhesion kinase activation loop

phosphorylation in nascent and mature focal adhesions and requirement for

the autophosphorylation site". Cell Growth and Differentiation (2000) 11:

41-48.

6. Karmaker, S., Ghosh Chaudhuri A. and Ganguly U. "Comparison of cytosolic

levels of calcium and G actin in diffuse and localised adherent Escherichia

coli-infected HeLa cells". FEMS Microbiol Lett (1996) 135: 245-249.

7. Karmaker, S.,Ghosh A.N., Dey D., Nair G.B. and Ganguly U.

"Ultrastructural changes in HeLa cells associated with enteroadherent

Escherichia coli isolated from infants with diarrhea in Calcutta". Journal

of Dia. Dis. Res. (1994) 12(4):274-278.

8. Karmaker, S., Biswas D., Shaikh N.M., Chatterjee S.K., Kataria V.K. and

Kumar, R. "Role of large plasmid of Salmonella typhi encoding multiple drug

resistance". Journal of Medical Microbiol. (1991) 34: 149-151.

EDUCATION

2010- Certificate course on "How to conduct Clinical Trial" from Clinical

Research Training Online Inc., Florida, USA.

2009- Present Doing Internship as part of Clinical Research Associate

certificate course from Kriger Inc

2009- Certificate course on Clinical Research Associate from

Kriger Inc.

2007 - 2008: Certificate course on Regulatory Affairs from San Diego State

University, California.

2003 - 2004: Attended Bioinformatics courses at Foothill College,

California.

1997: Ph.D. (Biophysics & Molecular Biology), University of Calcutta,

Calcutta, India.

1989: M.S. (Biophysics & Molecular Biology), University of Calcutta,

Calcutta, India.

1987: B.S. (Physiology), University of Calcutta, Calcutta, India.

AWARDS & HONORS

Senior Research Fellowship from Council Of Scientific & Industrial 1995-1996

Research (CSIR), India.

Senior Research Fellowship from Indian Council Of Medical Research 1993-1995

(ICMR), India.

Junior Research Fellowship from Indian Council Of Medical Research 1991-1993

(ICMR), India.

Passed the Graduate Aptitude Test in Engineering (GATE), an 1991

All-India Examination to get admission to PhD program.

Qualified in the joint CSIR-UGC exam for lectureship in Life 1989

Sciences, India.

AFFILIATIONS

1. Life member, Indian Science Congress Association.

2. Member, American Society of Cell Biology.

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