High School Technician

Eileen A. Larkin

*** *** ***, ***. ** . Frederick, MD 21702

215-***-**** . ******.**********@*****.***

Research Scientist

Highly skilled professional with strong background in laboratory research

and expertise planning, executing, and documenting well-designed

experiments. Assist in developing and revising lab documents, training

researchers, and identifying opportunities to optimize lab performance.

Thrive in collaborative team environments while maintaining sense of

ownership of projects. Prioritize projects effectively and balance

concurrent responsibilities with ease. Exhibit excellent analytical and

problem solving abilities; display unwavering commitment to quality,

accuracy, and reliability of investigations, data, and experiments.

Dedicated, valued, and respected associate recognized for superior work

ethic, strong attention to detail, and innovative approach to problem

solving. Pursuing MS in Biomedical Sciences; anticipated completion in

September 2010. Proficient with various molecular and cell biology

techniques including flow cytometry, radiation techniques, and FPLC.

Additional lab skills include:

Tissue Culture Cell line maintenance, aseptic technique, and cell culture

assays, including T cell proliferation, Inhibition

Concentration 50 percent (IC50s), and therapeutic assays.

Tissue Ficoll separation of blood culture, cell separation from

Processing other tissue samples

Protein ELISA, Gel electrophoresis and staining, Western Blot,

Interaction Protein Microarray, and Surface Plasmon Resonance

Techniques

Bacterial Plate Streaking, Colony picking/counting, Transfection,

Culture Transformation, and DNA/RNA/protein harvest

Small Animal Mice care and small animal handling, injections,

Testing euthanasia, transport, and sample collection (blood,

saliva, nasal, and organs)

Professional Experience

U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD

. 2006-Present

Graduate Fellow/Lab Technician, Integrated Toxinology Department

Perform broad range of functions to support daily laboratory operations.

Specialize in characterization of monoclonal antibodies as potential

diagnostic and therapeutic agents against bacterial superantigens.

Process specimens and ensure adherence to GLPs, study protocols, and

other applicable standards. Maintain inventories and calibrate equipment

to ensure reliability and validity of information. Assist student interns

with research projects and provide training on immunology and other

biological techniques.

. Established position as subject matter experts on microarray

development; created and optimized two protein microarray assays now

used by others in lab and institute-wide.

GEAR UP Forensics Academy - Shippensburg University, Shippensburg, PA .

June 2006

Counselor

Organized and facilitated activities and experiments for middle school

students at forensic science camp; demonstrated fingerprinting and DNA

isolation techniques using samples from cheek swabs.

Cumberland County Justice Department Crime Lab, Carlisle, PA . February-

April 2006

Intern

Tested evidence for presence of illegal narcotics. Ensured validity,

accuracy, and reliability of all information and maintained database.

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Eileen A. Larkin

215-***-**** . ******.**********@*****.***

Education, Honors, & Civic Involvement

Master of Science in Biomedical Science (Candidate) . Hood College -

Frederick, MD

Anticipated completion September 2010

Bachelor of Science in Biotechnology . Shippensburg University -

Shippensburg, PA

SciTech Scholarship (PHEAA Nets) Recipient, 2003-2006 . Dean's List, 2002-

2005

Volunteer with FIRST Robotics Competition, 2002-Present

Publications

Larkin, E.A., Stiles, B.G., Ulrich, R.G. "Immunotherapeutcis Against

Staphylococcal Toxic Shock Targeting T-cell Receptor Interactions with

Exterotoxin B." Submitted to J. Biol. Chem., 2010.

Larkin, E.A., Krakauer, T., Ulrich, R.G., Stiles, B.G. "Staphylococcal and

Streptococcal Superantigens: Basic Biology of Conserved Protein Toxins."

TOTJ. 3(81): 1-81, 2009.

Larkin, E.A., Carman, R.J., Krakauer, T., Stiles, B.G. "Staphylococcus

aureus: The Toxic Presence of a Pathogen Extraordinaire." Curr. Med. Chem.

16: 4003-4019, 2009.

Presentations

"Human Monoclonal Antibodies against Staphylococcal Enterotoxin B:

Potential Therapeutics?"

Seminar. Novel Therapeutics for Biothreat Agents and Emerging Pathogens

Series. National Cancer Institute, Frederick, MD, November 2010.

"Human Monoclonal Antibodies Against Staphylococcal Enterotoxin B." Poster

session. 109th Annual American Society of Microbiologists General Meeting.

Philadelphia, PA, May 2009.

"Hooray for Arrays: Analysis of the Superantigen Induced Immune Response by

Cytokine Microarray."

Poster session. NCI Frederick and Fort Detrick Spring Research Festival.

Frederick, MD, April 2009.

"Science is Fun!: An Introduction to the World of Biodefense." Seminar.

Honors Biology Classes. Lansdale Catholic High School. Lansdale, PA, March

2009.

"Human Monoclonal Antibodies against Staphylococcal Enterotoxin B:

Potential Therapeutics?" Poster session. NCI Frederick and Fort Detrick

Spring Research Festival. Frederick, MD, May 2008.

"Human Monoclonal Antibodies against Staphylococcal Enterotoxin B:

Potential Therapeutics?" Poster session. 108th Annual American Society of

Microbiologists General Meeting. Boston, MA, June 2008.

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Eileen A. Larkin

215-***-**** . ******.**********@*****.***

Technical Proficiencies

Proficient with Microsoft Office Suite, Adobe Suite, PyMOL, BIAEvaluation,

Unicorn (FPLC analysis), and AIRS ordering system.

Skilled in utilizing the following laboratory software applications and

instruments:

. GE Healthcare BIACORE 3000 and FLEXChip surface plasmon resonance

instruments

. FACS Calibur

. Array Jet Marathon and BioRad BioOdyssey Calligrapher microarray

spotters

. GenePix microarray imager

. BioRad VersaDoc Imaging System

. Perkin Elmer Filtermate Harvester (cell harvest)

. TopCount NXT (radiation counter)

. Perkin Elmer Victor 3V (fluorescence scanner)

. Nexcelon Biosciences Cellometer Auto T4 (Cell counter)

. ELx405 Select 96 well plate washer, and AKTA FPLC system.

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