Technician Maintenance

Anjum S. Manashiya

*** ***** * ******* ****. South, Harrison, NJ - 07029 ? 973-***-**** / 973-

***-**** ? ***********@*****.***

SUMMARY

Obtain valuable position in esteemed organization where I can utilize my

education, knowledge, professional work experience and research for

accomplishing the projects in the field of biotechnology. Proven ability

to apply general principles of research and laboratory techniques and

provide feasible solutions to problems.

PROFESSIONAL EXPERIENCE/RESEARCH

NYU Langone Medical Center, NY Jan 2010- Till Date

Associate Research Technician, Clinical Pharmacology Dr Bruce N.

Cronstein and Hailing Liu

. Experienced in Cell culture techniques, cell maintenance and cell

based assay including preparation of cell culture systems, passaging

cell culture, cell counting using haemocytometer, preservation of cell

culture, freezing and thawing cells.

. Achieved skills in RNA and Protein isolation from human cells.

. Excellent skills in SDS-PAGE electrophoresis to separate proteins

based on their electrophoretic mobility.

. Achieved experience in Western Blotting and analyzing results using

Molecular Imaging Software.

. Excellent skills in qRT-PCR and analyzing results using MxPro -

Mx3005P (Standalone) SYBR Green Software.

. Experienced with animal (mice) handling.

. General Lab inventory and protocol generation skills.

Sanofi Pasteur, Swiftwater, PA Jun 2009 - Dec 2009

Internship (Lab Technician), Analytical Immunology, R&D

Balasubramanian Arun and Patricia Greene

. Design and assay development of immunological experiments using

different ELISA techniques.

. Achieved knowledge and experienced in Biacore for measuring protein-

protein interaction and binding affinity, determination of active

concentration, screening and antibody characterization.

. Experienced in Cell culture (mammalian cells), media preparation, cell

maintenance, and analyzing results in support of method.

. Product characterization, generation of protocols and reports, assay

transfer to other departments.

. Maintaining lab equipments, calibration of liquid handling systems,

monthly cleaning of centrifuge, incubators, hoods, freezers, pH meters,

water baths and balances while following company policy and standard

operating procedures (SOP) including GLP and GMP.

Graduate Research Assistantship - Marywood University, PA Aug

2008 - May 2009

Clostridium perfringens Dr. Sayeed Sameera

. Investigation of Clostridium perfringens in the soil samples to find

the isolates which carry toxin genes.

. Research experience involved general laboratory safety, media

preparation, plating, screening, polymerase chain reaction, gel

electrophoresis, gel extraction and purification, genetic sequence

analysis, bacterial cell culturing, and genetic cloning.

EDUCATIONAL QUALIFICATION

MASTER'S DEGREE IN BIOTECHNOLOGY (Professional Science Degree)

Marywood University, Scranton, PA Aug 2008 - Dec 2009

GPA: 3.75

Research Methods and Statistics (SPSS), Cell Culture, Business Venture

and Entrepreneurship, Marketing and Strategic Planning, Management of

Innovation and Technology, Emerging Medicines and Technology, Ethics in

Management, Public Presentation and Literature Review.

MASTER'S DEGREE IN BIOTECHNOLOGY

T. John college, Bangalore, India Aug 2005 - July 2007

Grade: First Class

Biochemistry, Molecular Cell Biology, Genetics, Bioinformatics,

Biostatistics, Animal and Plant tissue Culture, Microbiology, Immunology,

Immuno-Technology, Fermentation Technology, Protein Structure and

crystallization, Biotherapeutics. (Practical and Theory)

BACHELOR'S DEGREE IN LIFE SCIENCES, Jai hind college, Mumbai, India

May 2002 - March 2005

Grade: First Class

SKILLS SUMMARY

. Tailored screening strategies using ELISA, immunoblot techniques,

automated liquid handling systems and their calibration.

. Cell culture, cell maintenance and good working knowledge of immunology

and Biochemistry.

. RNA/DNA and Protein isolation, qRT-PCR, multiplex PCR, gel

electrophoresis, SDS-PAGE, Western Blotting, colorimetric analysis,

chromatography, staining techniques, tissue culture techniques,

immunological techniques, mass spectrometry, lyophilization and

enzymatic estimations.

. Histochemical Staining.

. Proficient in Power Point, Microsoft Word and Excel.

. Proficient in Softmax Pro Software, MxPro - Mx3005P (Standalone) SYBR

Green, KODAK MI.exe - Molecular Imaging Software.

SHORT TERM CERTIFICATE COURSES/ TRAININGS

. ELISA and Clinical Biochemistry conducted by "Agappe Diagnostic Pvt.

Ltd"

. Participated in symposium organized by T. John College, Bangalore,

India-Feb 2007

. Training form Sanofi Pasteur on GLP, GMP and SOP form June 2009 - Dec

2009

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