Maintenance Training
Resume:
ARUNA. K
**** * ******** ******, *** ***, Chicago, IL 60616
****.*****@*****.***
OBJECTIVE
Seeking a biology research position that will effectively maximize my
experience and skill set in cell culture and protein purification.
PROFILE
I have 3+ years of combined experience in Industry and Academic research. I
have worked both as an independent researcher and as a team player to
perform experiments in optimizing protocol in upstream which broadly
includes design, operation and maintenance of bioreactor, cell culture,
scale up, harvesting cell pellet and inclusion body. My job extensively
involved troubleshooting bioprocess parameters involved in scale up such as
maintaining optimal dissolved oxygen level, pH level of culture. I have
worked in a cGMP regulated company in compliance with FDA for recombinant
protein production. I have worked in clean rooms (Class 10000, 100000) and
well versed with preparation of batch records and standard operating
procedures. I have prepared documents and training for operation,
calibration and maintenance of bioreactor (wave 10/20, 20/50). I have also
held management position and handled a team of 30 members for projects
concerning alternative method of treatment and this job demanded extensive
market research and document preparation.
EDUCATION
Master of Science (Biological Science), Illinois Institute of Technology,
Chicago, IL
Bachelors in Technology (Industrial Biotechnology), Anna University, India
EXPERIENCE
Therapeutic Proteins Inc., Chicago, IL
Position - Scientist Grade II (October 2009 to October 2010)
Team Size - 3
Microbial cell culture and scale up using Wave Bioreactor- 10/20, 20/50EH
of E. Coli producing Granulocyte Colony Stimulating Factor
Have practiced sterilization techniques such as autoclaving, filter
sterilization and screened colonies using selective marker
Designed bioreactors (GE wave bags) based upon specific requirement during
scale up such as sparge rod loop design for enhancing dissolved oxygen
level, setting up water trap for air outlet.
Designed and monitored bioprocess growth parameters such as optical density
of starter culture, optical density of scaled up culture, pH, dissolved
oxygen, agitation (such as optimal angle, rpm in wave bioreactor)
Performed plasmid extraction, purification, transformation of bacteria
Prepared GMP regulated cell bank in clean room (Class 10,000)
Have obtained cell pellet, inclusion body by homogenization followed by
centrifugation
Involved in validation of equipment, buying equipment, working with QA
Prepared SOPs, batch records for cell culture process and equipment in
compliance with GMP practices
Written, executed and assisted any validation or calibration projects
related to the upstream operation and equipment maintenance
Clean room maintenance, Class 10K, 100K- Cleaning, particle counting, and
documentation
Can individually operate analytical instruments like HPLC (Agilent
1100/1200), UV- Visible spectrophotometer, SDS PAGE (reducing, non-reducing
condition) and western blot.
Have training and understanding of mammalian cell culture (CHO DG44 cells)
- Operating CO2 incubator, checking cell viability count (Countess
automated cell counter), cryopreservation.
Obtained basic level training in operating FPLC (AKTA) and Tangential Flow
Filter.
Pritzker Research Institute, Illinois Institute of Technology, Chicago IL
Position- Research Associate - (January 2008 to October 2009)
Worked with animal samples such as rat tail tendon, lamprey notochord
during collagen extraction.
Performed isolation and purification of Type I and Type II collagen fibril
by novel biochemical method (Patented by lab), salt extraction, dialysis
and centrifugation.
Performed biophysical studies on collagen fibers by Atomic Force
Microscope, X-ray diffraction.
Crystallographic study of Type I, Type II collagen by X-ray diffraction.
IQ Ltd, Chennai, India
Position- Senior Medical Information Technologist, (October 2006 - August
2007)
Team Size - 30
Prepared and edited reports on alternative methods of treatment. The job
involved extensive online research, collecting latest papers published in a
specific domain, understanding and compiling all relevant data into a
single report to create an online database.
Extensively used open source such as BLASTA, FASTA, UNIPROT
Have been part of recruiting and training candidates.
COMPUTER SKILLS
ChemStation (HPLC)
SPOCK (3D protein structure)
FIT 2 D (x-ray)
Nanoscope (AFM)
Molecular Imaging (AFM)
Microsoft Office Tools Suite
PUBLICATIONS
Zhu J, Sabharwal T, Guo LH, Kalyanasundaram A, Wang GD,. 2009. Gloss
Phenomena and image analysis of atomic force microscopy in molecular and
cell biology. Scanning, 31:55-64
Jie Zhu, Tanya Sabharwal, Aruna Kalyanasundaram, Lianhong Guod, Guodong
Wang., 2009. Topographic Mapping and Compression Elasticity Analysis of
Skinned Cardiac Muscle Fibers in Vitro with Atomic Force Microscopy Journal
of Biomechanical engineering, 42(13): 2143-2150.
AWARDS
Dean's research scholarship, 2009 - Illinois Institute of Technology,
Chicago, IL
Best Outstanding Student of Department of Biotechnology, 2006 - Anna
University, India
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