RENALDO ISAAC
CURRENT ADDRESS E-MAIL
**** ******** **** ******@***.***
Raleigh, NC 27610
OBJECTIVE
To obtain full time employment working in a lab setting where I am able to utilize my skills in microscopy, cell
culturing, media preparations, analysis, and technical writing.
CAPABILITIES
Proven capabilities are applicable are in lab setting, including:
Microscopy Extraction Distillation
Cell Culturing Mass Spectrometry NMR Spectroscopy
IR Spectroscopy Qualitative Analysis DNA digestion (Via Endonucleases)
Gel Electrophoresis Chromatography (Gas and TLC) PCR
Cryo-genetic Cell Storage Flow Cytometry MTT Proliferation Assay
EDUCATION
Bachelor of Science in Biology, Concentration: Molecular Biology, and Biotechnology,
East Carolina University,
Graduation date: July, 31 2009
Greenville NC
SELECTED EXAMPLE OF JOB-RELATED COURSEWORK
Cell Culturing Workshop
My most recent lab experience came during the summer of 2009, when I took a 4 week workshop at East Carolina
University designed to give students hands on experience cultivating cells. In the workshop we grew human
epithelial kidney cells, and mouse myoblast cells, that we used for to learn other lab techniques such as: Cell
counting “via” hematocytometer, vector infection, RNA isolation, and uses of a Flow Cytometer.
DNA Digestion of PUC 19 DNA
In biological techniques lab, we cleaved circular PUC 19 DNA that we extracted from cultures of bacterial cells.
This DNA was then digested by two Endonucleases (EcoR1, Tag1,). The cleaved DNA was then migrated. In an
agarose gel, via Gel Electrophoresis, and then photographed to examine different strand sizes, and migration
lengths.
Extraction Lab Practical
In organic chemistry lab, we were required to create and carry out an experimental procedure for an acid-base
extraction. I reviewed the experiments we had conducted previously, conceived a procedure similar to those, and
carried out the assignment in a relatively short amount of time. Upon completion, I discovered that my percent
yield (by weight) and purity (based on melting point) was among the highest in the class.
Chemical Literature Assignment
In organic chemistry lab, we were required to identify, find the structure of, and reference several different
ingredients from an everyday product. I researched the active ingredients of Macchar Mosquito Repellent. I
learned how to use SciFinder Scholar and other resources in East Carolina University Joyner Library to find
information on organic compounds.
RENALDO ISAAC
RESUME CONTINUED PAGE 2
******@***.***
Thin Layer Chromatography Experiment
In organic chemistry lab, we were required to identify the active components in a non-prescription analgesic drug. I
carried out this identification using thin layer chromatography (TLC) to compare the analgesic with a set of known
reference compounds. I determined, qualitatively and quantitatively (using Rf values), that the unknown analgesic
drug was aspirin (acetylsalicylic acid)
MAJOR COURSES COMPLETED
Microbes and Immunity, Microbiology, Cellular Physiology
Principles of Biochemistry 1&2, Principles of Genetics, Molecular Genetics
Immunology, Ecology, Basic Laboratory Methods
Cell Culturing, Organic Chemistry 1&2, Marine Biology
ACCOMPLISHMENTS
Accomplishments, awards, and honors include: three semesters on the National Dean’s List and Two-year All
Conference USA Long Jumper for the East Carolina University Track Team.
COMPUTER SKILLS
Operating Systems and Networking- Windows 98,200, and XP
Software- MS Office (Word, Excel< Power Point, AutoCAD, and Publisher)
EXTRACURRICULAR ACTIVITES
On and off campus extracurricular activities at East Carolina University: I have participated,
volunteered and collected donations for a number of non-profit organizations including:
March of Dimes, Port City Wheelers Motorcycle
Van Club ride for Sickle Cell Anemia
Red Cross Blood Drive
Mentoring for the YES Program for at risk males/females between the ages of 12-18 years of age.
PROFESSIONAL CHRONOLOGY
Gold’s Gym, Raleigh, NC 2008-Presant
-Customer Service Rep. /Front Desk: Duties include opening and closing the gym, answering phone, assisting
customers with bill payments, account issues, and general questions about memberships and policies, also assist
management with bill collection
Convergys, Greenville, NC 2006-2007
-Telephone Customer Service Rep.: Convergys is a company that does telephone customer service for different
companies, my particular department worked under Charter Cable. Customers would call the customer service line,
and I would help them start new accounts with Charter Cable, make payments on accounts, close accounts, and
assist with general service changes
Del Labs, Castle Hayne, NC 2002-2003
-Package Handler: Del Labs is the company that packages and ships Sally Hansen Cosmetic products. My job
was to label the packages with the correct shipping label, and load pallets on the trucks so that they can depart on
time.
REFRENCES
Mary Farwell, PhD David Schwab Willie L. Barnhill
Professor and Director of- General Manager Assistant Deputy OPS
Undergraduate studies Gold’s Gym Combat Logistic Force
East Carolina University 919-***-**** 202-***-**** DSN 325