Assistant Development
Resume:
Jerzy Wojciechowski
Windsor, ON N8W 5T7
Phone: 519-***-**** (Home)
E-mail: *************@******.**
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Objective:
I am actively seeking a position as a scientist in either a R&D or QC department of
biotech/pharmaceutical company or biological-chemical diagnostic laboratory. The position of a technical
consultant/advisor in the field of broad Life Sciences allowing me to use my interdisciplinary expertise is
also desirable.
Summary:
Interdisciplinary background in Chemistry, Chemical Engineering and Life Science, including Medical
Science. M.Sc. in Chemistry and Chemical Engineering; M.Sc. in Biochemistry.
Experienced in most modern analytical techniques employed in research laboratory with either chemical
or biological profile as well as in methods development. DNA & protein microarray
technology and bioinformatics.
Expertise in the design, construction and usage of phage display technology used for serum- based
biomarkers for various tumors. Data mining and bioinformatics. Working knowledge of
GLP/GMP. Multilanguage proficiency.
Superb interpersonal skills. In-depth technical expertise on operation and troubleshooting of sophisticated
laboratory equipment.
Employment History:
Karmanos Cancer Institute, Wayne State University, Detroit, MI (February 2000- present)
Research Scientist
Responsible for assay set-up, development and implementing DNA and protein microarray technology
(cDNA & Affymetrix).
Development of phage display technology in context of search for serum-based tumor biomarkers for
early cancer detection. Libraries preparation (several high quality cell-line- and tumor-
based libraries have been synthesized) and biopanning.
Protein chips production (spotting and assay set-up).
Maintenance, operating and troubleshooting of chip printer as well as other peripheral devices, such as
fluorescent scanner, BIOMEK, etc.
Data mining & analysis, bioinformatics.
IntelliGene Expressions, Inc., Edmonton, AB (1998-2000)
Research Assistant
Responsible for protein purification and analysis of final products (HPLC, Western blot, ELISA, IEF)
Department of Cell Biology and Anatomy, University of Alberta, Edmonton, AB (1997-1998)
Research Assistant/Graduate Research Assistant
Molecular biology of yeast peroxisome biogenesis.
Department of Biochemistry, University of Western Ontario, London, ON (1992- 1997)
Research Assistant/Graduate Research Assistant
Member of research group working on cholesterol metabolism in rats.
Responsible for methods development and chemical/biochemical analysis using gas chromatography,
HPLC, column chromatography, thin-layer chromatography, UV, IR, VIS spectroscopy, analytical
techniques employing isotopic tracers. Preparation of scientific reports & presentations, library search.
Laidlaw Environmental Service, Thorold, ON (1990- 1992)
Chemist
Conducting analysis of industrial liquid and solid wastes (gas chromatography, UV, IR, VIS spectroscopy,
electrochemical methods of analysis, wet chemistry).
Regional Environmental Control Station, Opole, Poland (1979-1990)
Research Chemist
Responsible for sampling and analysis of industrial water, liquid and solid wastes and air pollutants (gas
chromatography, UV, IR, VIS spectroscopy, atomic absorption spectroscopy, electrochemical methods,
wet chemistry); methods development.
Statistical analysis of environmental pollution in the region; preparation of technical reports.
Supervising 5 laboratory technicians.
Summary of skills:
Analytical Chemistry: GC, HPLC, TLC, column chromatography,
UV/IR/VIS/AA spectroscopy, classical methods, including wet chemistry, electrochemical
methods, isotopic measurements; methods development.
Biochemistry & Molecular Biology:
DNA/ RNA extraction & analysis, restriction mapping, hybridization, Southern & Northern blotting, cloning
techniques (E.coli, yeast), sequencing, PCR, RT-PCR.
Protein extraction and purification, Western blotting, ELISA, IEF, enzymology.
Microbiology & aseptic techniques.
Experience in animal care (rats & mice), small animal surgery, tissue cell cultures.
Processing and handling serum and other fluids.
DNA and protein microarray technology; phage display technology:
Expertise in the design, construction and usage of phage display library construction. Phage based- and
protein chips (printing, analysis; assay set-up), cloning techniques in various systems (bacteria, yeast) ;
High throughput DNA and protein sequence analysis;
cDNA chip and Affymetrix;
Bioinformatics:
Clustering and data mining tools: SpotfirePro, GeneSpring, EPICLUST, BioDiscovery, Partek; Web-based
molecular biology tools and data bases, etc..
Working knowledge of GLP/GMP.
Statistical management of experimental data using data analysis programs (Sigma Plot, S-Stat, MatLab,
etc.); statistical tools employed in microarray technology .
Biological data visualization, biological network analysis. Knowledge of major public biological databases.
Advanced computer literacy; familiarity with most utility software; data mining; programming languages:
R.
Computer platforms: Windows, Windows NT, UNIX,
Other skills:
Proficiency in writing of technical reports and making presentations.
Teaching experience: tutoring and TA in undergraduate course in Biochemistry (University of Western
Ontario).
Languages written and spoken: Polish, Russian, Dutch, German
Technical experience:
Management, programming and operation of sophisticated laboratory equipment (BIOMEK, microarray
printer, fluorescent scanner)
Professional Accomplishments:
Significant contribution to the patent issued in the name of the University of Western Ontario in 1997.
Contribution to the patent (serum-based biomarkers for breast cancer) issued for the Wayne State
University in 2007.
Development of new original methods of separation and purification of oxysterols (TLC, column
chromatography).
Education:
University of Western Ontario, London, ON
M.Sc. Biochemistry (1997)
“Evidence of inhibition of cholesterol absorption in rats by naturally-occurring oxysterols”
Academy of Medicine (currently- School of Medicine, University of Wroclaw), Wroclaw, Poland
Medical School (1984-88); completed 2/3 of medical degree.
Technical University of Silesia, Gliwice, Poland
B.Sc.,M.Sc. Eng. Chemistry and Chemical Engineering; Technology of Polymers (1979)
Publications:
Pauley, RJ, Santner, SJ, Wojciechowski, J., Tainsky, MA, Tamoxifen and estrogen effects on gene
expression in prenoplastic MCF-10AT and neoplastic MCF-7 cells, abstract, 94th Annual
Meeting AACR, 2003, Toronto, ON, Canada
Lin, H.-S., et al., Autoantibody approach for serum-based detection of head and neck cancer, Cancer
Epidemiol Biomarker Prev., 2007, 16, 2396;
Wojciechowski, J., et al, Proteomic approaches to early detection of breast cancer, poster presentation,
Annual EDRN Meeting, Washington, DC, 2004.
Chatterjee, M., Wojciechowski, J., Tainsky, M.A., Discovery of antibody biomarkers using protein
microarrays of tumor antigens cloned in high throughput, chapter in “Tumor Biomarker
Discovery”, Tainsky, M.A. (Editor), 2009,Humana Press.
Dudas, S., Wojciechowski, J., On-chip diagnostic immunoassays of panels of bioselected tumor antigens:
binding to serum autoantibodies for the early detection of cancer, poster presentation,
Annual EDRN Meeting, Washington, DC, 2009
References Available upon request
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