Medical Research

Curriculum Vitae

Weilin Wu, Ph.D.

Staff Scientist

The Scripps Research Institute

Jupiter, FL 33458

Phone: 703-***-**** (cell), 561-***-**** (lab)

Email: ********@*******.***

EDUCATION

Ph.D. Molecular Biology 2002

German Cancer Research Center (DKFZ), University of Heidelberg,

Germany.

M.S. Biochemistry 1997

Dept. of Biochemistry, Chinese Academy of Medical Sciences (CAMS)

& Peking Union Medical College (PUMC), P.R.China.

B.S. Biology 1994

College of Life Sciences, Hunan Normal University (HNU), P.R.China.

RESEARCH EXPERIENCE

Staff Scientist 2008.05 - present

Dept. of Cancer Biology, The Scripps Research Institute, Jupiter, FL.

Research focused on cancer stem cell:

1) Differentiation and regulation of stem cells;

2) Identification of small chemical molecules in inhibiting specific

signaling pathways.

Research Assistant Professor 2007.02 - 2008.05

Dept. of Biochemistry and Molecular Biology, George Washington University,

DC.

Research focused on HIV:

1) Mechanism of small chemical molecules in inhibiting HIV infection;

2) ChIP-on-CHIP study of genome profiling altered by HIV infection with

BRG1 and CREB1.

Postdoctoral Fellow 2002.12 - 2006.12

Center for Genomics and Bioinformatics, Karolinska Institute, Stockholm,

Sweden.

Research focused on RNAi: High-throughput screening for novel gene

functions using RNAi in mammalian cells.

Postdoctoral Fellow 2002.02 - 2002.09

Beth Israel Deaconess Medical Center and Harvard Institute of Proteomics,

Boston, MA.

Research focused on:

1) Regulator of G-protein signalling, Xain/XARP and apoptosis-inducing

factor;

2) High-throughput cloning, expression and purification of Pseudomonas

substrates of histidine protein kinasess.

Ph.D. Student 1998.08 - 2002.01

Division for Molecular Biology of Mitosis, German Cancer Research Center,

Heidelberg, Germany.

Ph.D. thesis work: "Identification and characterization of proteins

interacting with RanGAP".

Research Staff 1997.05 - 1998.08

National Key Laboratory of Medical Molecular Biology, Chinese Academy of

Medical Sciences (CAMS) & Peking Union Medical College (PUMC). Beijing,

China.

Research focused on:

1) Genetic engineering of analogues atrial natriuretic peptide, SNAP1 (an

ANP analogue) gene cloning and mutation assay;

2) Tagged protein expression and purification, and in vivo functional test

of SNAP1 in mice.

Graduate Research Assistant 1994.09 - 1997.05

Dept. of Biochemistry, Chinese Academy of Medical Sciences (CAMS) & Peking

Union Medical College (PUMC)/Institute of Blood Transfusion. Chendu, China.

MS thesis work: "Thermodynamic mechanism of the stability of protein under

heat-treatment".

RESEARCH EXPERTISE

Cell biology

1) CHIP-on-Chip study on gene expressions during cancer development;

2) Isolation of stem cells; differentiation and regulation of stem cells;

3) Identification of small chemical molecules inhibiting specific

signaling pathways;

4) Cell culture: highly professional in different kind of cell culture

systems and highly creative in developing different kinds of assays.

Cell-based assay

1) High-throughput cell-based assay using reporter gene system, such as

GFP, luciferase, etc.

2) Large-scale gene knockdown (RNAi) and tagged gene over-expression and

functional analysis, such as cell proliferation, cell death, etc.

3) Highly proficient in different gene silencing techniques, such as

lentivirus-delivery, adenovirus-delivery, siRNA, shRAN and different

inducible shRNA system.

Protein science and molecular biology

1) 2-D/MS analysis;

2) ChIP-on-ChiP;

3) High-throughput from gene cloning to protein expression and

purification;

4) Protein interaction study: yeast two-hybrid, GST-pulldown, Co-IP, etc.

5) Highly professional in AKTA systems from small-scale to medium-scale

of protein purification.

FELLOWSHIPS AND AWARDS

Postdoc Fellowship 2002.12 - 2006.6

Karolinska Institute, Stockholm, Sweden.

Postdoc Fellowship 2002.02 - 2002.9

Harvard Medical School, Boston, MA.

Ph.D. Fellowship 1998.08 - 2002.01

German Cancer Research Center, Heidelberg, Germany.

Scholarship for Excellent Graduate Students 1996 - 1997

Chinese Academy of Medical Sciences & Peking Union Medical College,

Chengdu, China.

GRANT APPLICATION

Pending:

1) NIH R01, immune signaling and prostate cancer, 12/01/10-11/30/15 (Co-

investigator).

Completed:

2) Bankhead-Coley Cancer Research Program The kinase-independent function

of IKKa protein in breast cancer development, 07/01/09-06/30/10 (Co-

investigator).

JOURNAL REVIEW ACTIVITY

Ad hoc reviewer for Journal of Biological Chemistry, Journal of Proteome

Research, Journal of Virology, and Retrovirology. Invited reviewer for

Chemosphere, and Nucleic Acids Research.

CONFERENCE ATTENDENCE AND INVITED TALKS

1. "Targeting Cancer Invasion and Metastasis". Miami, Florida. Feb, 2010.

2. "Identification of miRNAs in the regulation of IKKalpha during stem

cell differentiation". The Scripps Research Institute, Florida. Sept,

2008. (Invited)

3. "Identification of an ER protein through RNAi screening that confers

resistance to microtubule targeted drugs". RNAi-2006-Boston Meeting on

RNA interference: Biochemical Genetics to Drugs & Therapeutics.

Boston, MA. May, 2006. (Invited)

4. "Identification of novel regulators affecting taxol-induced cell cycle

arrest and apoptosis by throughput RNAi screening on mammalian cells".

Nanhua University, Hunan, China. Nov, 2005. (Invited)

5. "Throughput validation of siRNA-induced cell death phenotypes confirms

new anti-apoptotic protein". Fudan University. Shanghai, China. Nov,

2005. (Invited)

6. "RNAi: powerful tool for functional genomics". Chinese Academy of

Medical School, Institute of Blood Transfusion. Chengdu, China. Nov,

2005. (Invited)

7. Proteomics: Technologies and Applications, Keystone, Colorado, USA.

March, 2004,

8. RNAi for pathway analysis. Karolinska Institute, Sweden. April 2004.

9. 10th CGB/CMB/LICR Conference Solbacka at Sundbyholms Slott, June,

2004.

10. Functional genomics, a Wallenberg Consortium North (WCN)

Minisymposium, KTH, Stockholm. Nov, 2004.

11. Proteomics in Cancer Research 3rd Rudbeck Symposium, Uppsala Uni,

Sweden. Nov, 2004.

SELECTED PEER-REVIEWED PUBLICATIONS

1. WL Wu, Kehn-Hall K, Pedati C, Zweier L, Castro I, Klase Z, Dowd CS,

Dubrovsky L, Bukrinsky M, Kashanchi F. 2008. Drug 9AA reactivates

p21/Waf1 and Inhibits HIV-1 progeny formation. Virol J. 18;5:41.

2. Van Duyne R, Easley R, WL Wu, Berro R, Pedati C, Klase Z, Kehn-Hall K,

Flynn EK, Symer DE, Kashanchi F. 2008. Lysine methylation of HIV-1 Tat

regulates transcriptional activity of the viral LTR. Retrovirology.

22;5:40.

3. Berro R, Pedati C, Kehn-Hall K, WL Wu, Klase Z, Even Y, Genevi re AM,

Ammosova T, Nekhai S, Kashanchi F. 2008. CDK13, a new potential human

immunodeficiency virus type 1 inhibitory factor regulating viral mRNA

splicing. J Virol. 82(14):7155-66.

4. J Xie, X Hua, T Zhuo, CQ Wu, Q Wu, HR Hu, F Xu, L Yu, M Zhang and WL

Wu*. SnoRNAbase: a comprehensive database for Small Nucleolar RNAs.

Nucleic Acids Res, 2007. D183-7.

5. WL Wu*, E. Hodges, C. H g. Throughput validation of siRNA-induced

cell death phenotypes confirms new anti-apoptotic protein. Nucleic

Acids Res. 2006.34(2).

6. WL Wu, E. Hodges, J. Redelius, C. H g. A novel approach for

evaluating the efficiency of siRNAs on protein levels in cultured

cells. Nucleic Acids Res. 2004. 32(2).

7. Van Duyne R, Cardenas J, Easley R, WL Wu, Kehn-Hall K, Klase Z, Mendez

S, Zeng C, Chen H, Saifuddin M, Kashanchi F. 2008. Effect of

transcription peptide inhibitors on HIV-1 replication. Virology.

5;376(2):308-22.

8. Agbottah E, Yeh WI, Berro R, Klase Z, Pedati C, Kehn-Hall K, WL Wu,

Kashanchi F. 2008. Two specific drugs, BMS-345541 and purvalanol A

induce apoptosis of HTLV-1 infected cells through inhibition of the NF-

kappaB and cell cycle pathways. AIDS Res Ther. 10;5:12.

9. Van Duyne R, Kehn-Hall K, Klase Z, Easley R, Heydarian M, Saifuddin M,

WL Wu, Kashanchi F. 2008. Retroviral proteomics and interactomes:

intricate balances of cell survival and viral replication. Expert Rev

Proteomics. 5(3):507-28.

10. E. Hodges, J. Redelius, WL Wu, C. H g. Accelerated discovery of novel

protein function in cultured human cells. Mol Cell Proteomics. 2005.

4(9): 1319-27.

11. T.V.S. Murthy, WL Wu, QQ. Qiu, Z. Shi, J. LaBaer, L. Brizuela.

Bacterial cell-free system for high-throughput protein expression and

a comparative analysis of E.coli cell-free and whole cell expression

systems. Protein Expr Purif. 2004, 36(2): 217-25.

MANUSCRIPTS TO BE SUBMITTED

1. WL Wu, M. Rokavec, Kedell, M. Conkright, Jun-Li Luo. Nuclear IKKa kinase

mediates breast cancer tamoxifen-resistance through up-regulation of

FAT10 (To be submitted to Nature Cell Biology).

2. WL Wu, M. Rokavec, S. Ding, Jun-Li Luo. Targeting cancer stem cells for

the treatment of hormone-refractory prostate cancer (To be submitted to

Nature).

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