Lab Data

Sally Yousif

****@**********.***

Cell: 831-***-****

EDUCATION

Georgetown University

Washington, DC

MS in Biotechnology, GPA: 3.7

May 2011

Related Course Work: Lab Applications of Biotechnology, Cell Culture Lab,

Fermentation Lab, Current Good Manufacturing Practices cGMP,

Bioinformatics, Intellectual Property

University of California, Davis

Davis, CA

B.S. Degree in Biotechnology, minor in Technology Management, GPA: 3.5

July 2010

Related Course Work: Organic Chemistry Lab, Biochemistry Lab, Biology Lab,

Immunology Lab, Physiology Lab, Anatomy Lab, Bioinformatics

SKILLS

Lab:

. ELISA, PCR, qPCR, Thermostable Helicase-Dependent Amplification

(tHDA), DNA extraction, Cloning, bacterial and mammalian cells

transformation and transfection, IR, UV, VIS Spectroscopy, column

chromatography, SDS-PAGE and Gel Electrophoresis, Northern, Southern,

and Western Blot, Primers & Protocol design

Software/ Computer Skills:

. Microsoft Office (Excel, Word, PowerPoint. Outlook), Python, BioEdit,

LIMS, Bioinformatics databases

Languages:

. Fluent in English, Arabic and Aramaic

WORK AND RESEARCH HISTORY

Associate Scientist II

May 2011-present

QIAGEN

Frederick, MD

. Work with Scientists and Researchers to analyze samples against a

targeted panel of mutations in genes with demonstrated links to cancer

using qPCR

. Develop new assays and improving existing ones through searching

literature and recent publications for somatic mutations related to

various cancers

. QC and validate new and existing somatic mutations assays on 96-well

and/or 384-well plate format

. Design and conduct experiments, record, analyze and present the data

to the research team. Write protocols and technical reports

. Update disease or pathway content through changing qPCR conditions,

primers and probes design

Intern, Research and Development

QIAGEN

January - May 2011

Gaithersburg, MD

. Worked with feasibility researchers on developing, optimizing and

automating of a molecular diagnostic assay that will be used to detect

Chlamydia and Gonorrhea (CT/NG) using HDA amplification technique

. Improved sample preparation, amplification and detection techniques

through identifying an evaporation barrier, proving its efficacy and

verifying it does not interfere with the HDA assay

. Designed and conduct experiments, record, analyze and present the data

to research team

Lab Assistant, Maselli's Lab

May 2009- July 2010

UCD School of Medicine, Neurology Department

Intern-Volunteer, Maselli's Lab

October 2008- May 2009

UCD School of Medicine, Neurology Department

Davis, CA

. Designed experiment on the best way to analyze various genes, looked

for mutations and their clinical impacts in patients with Myasthenia

Gravis

. Ran PCR and gel electrophoresis. Extract DNA from blood and agarose

gel. Measured DNA concentration using Nanodrops. Prepared DNA samples

for sequencing. Performed Bacterial transformation, restriction enzyme

digestions and plasmid purification.

. Analyzed data and sequences using BioEdit software, BLAST and the NCBI

website. Read, recorded and interpreted data in notebook and

designated worksheets and maintained mutations database. Designed

primers for new disease-related genes

. Prepared chemicals and agar palates. Helped other researchers and

technicians with various tasks around the lab

Food Safety Lab Analyst April-

September 2008

Primuslabs.com

Salinas, CA

Microbiology Lab Technician

May-October 2007

BSK Food and Dairy Labs

Salinas, CA

Trained according to Standard Operating Procedures

. Performed both qualitative and quantitative tests on leafy green

products, soil, fertilizers and water samples. Tasks: Logged in

samples and tested them for pathogens, ran PCR and ELISA.

. Recorded data on designated worksheets, analyze results, entered it to

a computer system-LIMS- and generate reports

. Prepared simple chemicals such as sterile media, agar and solutions

according to the established lab procedures, maintained and clean lab

equipment according to the instructions

. Learned about health hazards, proper sanitation methods, sterilizing

techniques, operating autoclave and proper biohazard waste disposal

Publications:

Maselli, R., Arredondo, J., Cagney, O., Mozaffar, T., Skinner, S., Yousif,

S., Davis, R., Gregg, J., Sivak, M., Konia, T., Thomas, K. and Wollmann, R., Congenital myasthenic syndrome associated with epidermolysis bullosa

caused by homozygous mutations in PLEC1 and CHRNE. Clinical Genetics, no.

doi: 10.1111/j.1399-0004.2010.01602.x

Leadership and Community Involvement:

Vice President, Biotechnology Program Class '11, Georgetown University: Sep

2010-May 2011

Volunteer at the American Red Cross Club, UC Davis Chapter

Sep 08-May 2010

Member of the California Community College Honor Society

Jan 2007-May 2008

References:

Holly Basham, MS, Qiagen, Gaithersburg

*****.******@******.***, Phone: 443-***-****

Jack Chirikjian, PhD, Georgetown University

***@**********.***, phone: 202-***-****

Ricardo Maselli, MD, University of California, Davis

*********@*******.***, phone: 530-***-****

Juan Arrendondo, PhD, University of California, Davis

************@*******.***, phone: 1-530-***-****

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