Medical Quality

Matthew P. Flagg

** ******** **.

Marlborough, MA 01752

Home phone: 508-***-****

Cell phone: 508-***-****

*******@**.***

OBJECTIVE: To be a part of a biotechnology endeavor as an entry-level

scientist, to continue developing my laboratory skillset - from basic

cloning to sophisticated FACS techniques - and to provide quality support,

ideas, and results. Skills include:

Cell Culture Vector modification FACS analysis

Cloning in E. Coli Cloning in CHO cell MS Office proficient

EXPERIENCE:

DeltaQuest Foundation, Inc. (Winter 2010)

A not-for-profit organization focused on quality of life (QOL) research,

DeltaQuest assesses the impact of medical treatments on patients' day-to-

day lives.

Response/Data Analyst

Researchers were attempting to detect shifts in patient perception of QOL

using data from two time points.

Coded patient responses to Multiple Sclerosis quality of life survey

Advised researchers about response trends

Genzyme

Using a time-tested developmental program, Genzyme is a biotech company

that pursues breakthrough treatments and innovative solutions that address

unmet medical needs.

Intern 2010 Summer (3 mos.)

Investigated several aspects of an in-house FACS clone sorting technique.

Helped to validate said technique in a trial with a second therapeutic.

Modified alternate start site responsible for FACS reporter

Scaled up reporter expressing clones from 96 well plate to shake

flakes

FACS analyzed transfected cultures to identify high producing pools

FACS analyzed individual clones in an effort to find high producers

faster

Intern 2009 Summer and Winter Break (4 mos.)

Assisted in basic platform research in Genzyme's therapeutic protein

expression (TPE) group.

Established comparability of cell growth in shake flasks and 24 well

shake plates

Removed one of two BamHI sites from a vector to facilitate future

therapeutic gene insertion

Maintained, froze down, and transfected CHO cells

Wolfe group at Boston University Medical Center (Summer 2008)

Intern

Investigated effect of various chemicals on glucose-dependent

insulinotropic polypeptide (GIP) signaling pathway.

Maintained M32 (mouse cancer) cell line for in vivo testing

Treated cells with various chemical conditions (ie. 30 min in rapamycin)

Made polyacrylimide gels, ran SDS PAGEs and gel to membrane transfers

Assayed various proteins (ie. pAKT) via Western Blotting

SeaEscape Kayak, Vinalhaven, ME (2000 - 2007)

Asst. Kayak Guide/Kayak Guide

Assistant guide on tours and in classes (6 years)

Planned, prepared, and guided day long tours without assistance (1

year)

Passed a Maine State Guide test, gained certification

LEADERSHIP:

Four Boston (2008-2011)

Councilperson/Group Leader (2010-2011)

Oversaw group of volunteers attending Boston Living Center, an HIV

positive shelter, and Seton Manor

Lead and organized weekly, hour long reflections

Planned, organized, and executed organization-wide events

Group Member

Volunteered at Seton Manor, a Section 8 housing unit for HIV+

individuals

EDUCATION:

Boston College, Newton, MA (Class of 2011)

Majors: Biology & English

Dean's First List (3.7 - 4.0) Spring 2009, Spring 2010, Fall 2010,

Spring 2011

Dean's Second List (3.5 - 3.69) Fall 2008 and Fall 2009

Dean's Third List Fall (3.3 - 3.49) 2007 and Spring 2008

Honors chemistry track

Honors biology track

Honors English track and thesis

3.65 GPA; cum laude

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