Stephen Kauffman
Pasadena, CA 91101
abg4bq@r.postjobfree.com
Laboratory Skills
I have several years of professional experience with the following
techniques:
IEF, PAGE, Western blot, immunopurification, HPLC, IC, UV-Vis assays,
fluorescence assays, ELISA, SPE, liquid-liquid extraction, and mass
spectrometry (ion trap, cyclotron, and quadrupole).
Employment History
Sep 2009 - Present: Analytical Chemist
Huntington Medical Research Institutes, Pasadena, CA
Analysis of biological samples (tissue, plasma, and cerebrospinal
fluid) for compounds associated with neurological conditions such as
Alzheimer's disease, Parkinson's disease, and chronic migraine.
Research is directed toward discovering the pathology and biomarkers
of neurological conditions. Specific responsibilities include setting
up assays, analyzing results using standard statistical tools, and
presentation of data.
Dec 2006 - Jul 2009: Research Associate
Anti-Doping Research Institute, Mar Vista, CA
Assisted senior research scientists in the development of new methods
for detection of performance-enhancing drugs. ADR is a nonprofit
institute founded by the former Director of UCLA's Olympic Analytical
Laboratory. Specific projects included detection of enkephalins in
serum, characterization of erythropoietic proteins using ion
chromatography, and detection of growth hormone in human urine. Work
on the latter project was published in a peer-reviewed journal.
Jun 2005 - Dec 2006: Staff Research Associate / Laboratory Assistant
UCLA Olympic Analytical Laboratory, Los Angeles, CA
Staff Research Associate: Screening of urine samples for
erythropoietic proteins using specialized isoelectric focusing /
Western blotting protocol, densitometric analysis of graphic data, and
compilation of results into laboratory reports.
Laboratory Assistant: Preparation of urine samples for GC/MS and LC/MS
analysis by addition of internal standard, pH adjustment, enzymatic
deglycosylation, liquid-liquid or solid-phase extraction, and chemical
derivatization.
Jun 1998 - Jul 2002: Radionuclide Chemist
Isotope Products Laboratories, Burbank, CA
Manufacture and calibration of radioactive sources for use in
radioactive metering devices.
Education
M.S. in Analytical Chemistry Jan 2008 - Jun 2010
California State University, Los Angeles
Graduate Research: Chemometric optimization of a liquid
chromatography method for characterization of benzo[a]pyrene (BaP)
photo-oxidation products. The photo-oxidation of BaP, a ubiquitous
carcinogen, occurs by least two distinct mechanisms and yields several
isomeric photoproducts. Analysis of these compounds in the
environment requires well-designed methods for sensitive and selective
detection. My project involved chemometric modeling of a response
surface of resolution and retention time against synergistic HPLC
factors, and resulted in complete optimization of the method.
B.A. in Music Sep 2002 - Jun
2005
California State University, Long Beach
B.S. in Chemistry
Loyola Marymount University, Los Angeles Aug 1993 -
May 1998
Awards & Honors
2010 Special Recognition in Graduate Studies, CSULA
2005 President's Honor List, CSULB
2005 Pi Kappa Lambda Honor Society, CSULB
2005 Phi Kappa Phi Honor Society, CSULB
1998 Outstanding Graduate in Chemistry, LMU
Volunteer Experiences and Professional Memberships
2010-present Member of the American Society for Mass Spectrometry
2004-2005 President, MTNA student organization, CSULB
1997-1998 Officer, Student Affiliates of American Chemical Society,
LMU
Selected Publications
1. Bane, T.K.; Timmons, M.S.; Kauffman, S.J.; Catlin, D.H. Enrichment and
immunoprecipitation of 22 kDa human growth hormone spiked into human
urine. Drug Test Analysis, 2009, 1, 441-446.
References
Krishna Foster, PhD
Professor & Graduate Research Advisor
CSULA Chemistry Department
5151 State University Drive
Los Angeles, CA 90032
abg4bq@r.postjobfree.com
Michael Harrington, MD
Molecular Neurology Program Director
Huntington Medical Research Institutes
99 N. El Molino Ave
Pasadena, CA 91101
abg4bq@r.postjobfree.com
Thomas Bane, PhD
Senior Scientist
Anti-Doping Research Institute
3873 Grand View Blvd
Los Angeles, CA 90066
abg4bq@r.postjobfree.com
Borislav Starcevic
Scientific Specialist
UCLA Olympic Analytical Laboratory
2122 Granville Ave
Los Angeles, CA 90025
abg4bq@r.postjobfree.com