Human Technician

Martin W.

Employment History:

****-**** **********, ************ **********, Dept. Veterinary Medicine,

University Minnesota.

****-**** **-******* ****** Wolf Corp. Company licensed, developed,

manufactured and assisted in marketing of proprietary membrane based cell

culture devices (CELLine). CELLine products currently marketed and sold

worldwide by Integra Biosciences, Sartorius Stedem, Becton Dickinson and

others.

1992-1994 CELLEX Biosciences. Research Scientist. Completed phase II

SBIR Grant for large scale expansion of human lymphocytes. Attained funding

of Phase I SBIR grant for a bioreactor tailored to bone marrow.

1991 Macalester College Visiting Lecturer Human Physiology, Dept. of

Biology

1989-1991 College of Saint Mary. Lecturer Human Physiology

1988-1992 Postdoctoral Fellowship. University of Minnesota, Department

of Laboratory Medicine and Pathology. NIH Fellowship: Study of CD10

Function in Hematopoiesis.

1980-1984 Laboratory Technician. University of Nebraska College of

Medicine. Dept. of Human Physiology and Biophysics. Department of

Internal Medicine.

Education:

1984-1988 Ph. D. Dept. of Human Physiology and Biophysics; University of

Nebraska College of Medicine.

1976-1980 B. Sc. Chemistry; Creighton University

Publications:

Expansion of activated lymphocytes obtained from renal cell carcinoma in an

automated hollow fiber bioreactor. Hillman GG, Wolf ML, Montecillo E,

Younes E, Ali E, Pontes JE, Haas GP Cell Transplant. 1994 Jul-Aug; 3(4):

263-71

Functional effect of IL-7-enhanced CD19 expression on human B cell

precursors. Wolf ML, Weng WK, Stieglbauer KT, Shah N, LeBien TW. J

Immunol. 1993 Jul 1; 151(1): 138-48.

Expression of a chimeric helix-loop-helix gene, Id-SCL, in K562 human

leukemic cells is associated with nuclear segmentation. Goldfarb AN, Wolf

ML, Greenberg JM. Am J Pathol. 1992 Nov; 141(5): 1125-37.

Development of a bone marrow culture for maintenance and growth of normal

human B cell precursors. Wolf ML, Buckley JA, Goldfarb A, Law CL, LeBien

TW. J Immunol. 1991 Nov 15; 147(10): 3324-30.

The response of splenic lymphocytes removed from hypophysectomized-

orchidectomized hamsters to phytohemagglutinin correlates with somatic

growth but not with circulating prolactin levels. Wolf ML, Campbell GT,

Blake CA. Endocrinology. 1990 Apr; 126(4): 2046-53.

Comparison of batch vs. Celline culture for production of mono-clonal

antibody in vitro as alternatives to ascites. Application: Murine

Hybridoma. M. Wolf; Technical Literature, Integra Biosciences Gmbh.

High density Cell Culture in a new passive membrane based bioreactor M.

Wolf, T. DeSutter; Biotechnology?International 1999

Antibody Manufacture in the Celline CL1000, Application: Murine

hybridoma M. Wolf; Technical Literature, Integra Biosciences. Gmbh.

Attachment dependent cell cultivation on Micro-Carriers: Secreted protein

production M. Wolf; Technical Literature, Integra Biosciences. Gmbh.

High density suspension culture for Recombinant Protein produc-tion from

CHO cells.M. Wolf; Technical Literature, Integra Biosciences. Gmbh.

Patents:

5,714,384 Compartmentalized tissue culture bag

5,707,869 Compartmentalized multiple well tissue culture plate

5,693,537 Compartmentalized tissue culture flask

5,462,874 Dialyzed multiple well tissue culture plate

Grants:

NIH SBR (Phase I) Bioreactor for Bone Marrow Cells, 1994

NIH SBR (Phase I) Enhanced Roller Bottle, 1999

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