Quality Assurance Control

Sirisha Dhavala

ab9ic9@r.postjobfree.com **** Alexandra Dr.

Ph: 405-***-**** Roseville, CA

Objective

Seeking industrial and/or research experience in the areas of biotechnology and biomedical engineering.

Summary

Experience in PCR, DNA and RNA extraction, Protein purification, ELISA, Western Blotting,

Chromatography techniques (SDS- PAGE, Gel exclusion, HPLC, Reverse Phase, Ion-exchange),

Gel and Capillary Electrophoresis.

Expertise in aseptic cell culture techniques of various cells like neuronal, stem cells, cancer and

mammalian cells.

Firsthand experience with Nano/Micro particle and Scaffold fabrication for biomedical and drug

delivery applications.

Expertise in nanoparticle and mineral characterization using FTIR and Mass Spectrometry.

Hands on experience with Photolithography (BioMEMS for biological applications), design and

fabrication of micro fluidic devices for studying cell growth and migration.

Proficient knowledge working with SEM, Profilometer, Spectrophotometer, Fluorescence and

Confocal microscope.

Working experience with Statistical research design and analysis methods and tools like SAS and

SPSS.

Experience with handling animals like rats for in vivo experiments.

Knowledge of cGMP, ISO, ICH and FDA guidelines.

Excellent communication skills (verbal and written), analytical and problem solving skills.

Worked in small and mid-sized teams and demonstrated effective team player and organizer

abilities.

Education

Master of Science in Biomedical Engineering Spring 2013

University of Texas, Arlington, TX. CGPA: 3.87 /4.0

Bachelor of Engineering, Biotechnology Spring 2011

GITAM, Andhra University, India. CGPA: 8.0 /10.0

Thesis

Design, Fabrication and Evaluation of a novel Microfluidic based in vitro Cell Migration Assay

Designed and fabricated a high throughput microfluidic platform for cancer drug screening.

Analyzed the therapeutic potential of metastatic inhibitors on hGBM

Compared the newly designed assay with standard cancer migration (scratch wound) assay with

specific focus on quantitative migratory behavior

Publications

Sirisha Dhavala, Samik Bhattarai and Young-tae Kim, Simple vacuum enabled filling fluids in

microchannels for cell based applications- submitted to Lab on a chip

SreeVidya Banda, Chetan Bhuwania, Sirisha Dhavala, and Young-tae Kim, Engineering

Enabled Biomimitic Corneal Stroma – in progress

Sirisha Dhavala and Young-tae Kim, Design, Fabrication and Evaluation of a novel Microfluidic

based in vitro Cell Migration Assay - manuscript ready

Work Experience

Neuroengineering Lab, Bioengineering, UT-Arlington and UT Southwestern Dallas, TX

Research Assistant Aug, ‘11 – Jun ‘13

Project lead responsible for design and characterization of microfluidic devices for application in

high throughput cancer drug screening.

Fabricated tissue engineered bone grafts and vascular grafts using collagen for biomedical

applications.

Involved in design, development and testing of biomimetic corneal tissue as an alternative to

current corneal transplants.

Used Solid Works to analyze the effect of mechanical stress and compression on the nature of

bone graft being designed in the laboratory.

Performed mechanical characterization studies (suture strength, shear stress, elasticity,

transparency) of the assembled constructs.

Performed cell culture, Immunostaining, cell based assays, tissue fixation and histology.

Conducted in-vivo and in-vitro studies to evaluate the engineered constructs.

Responsibilities also included cell line maintenance, data analysis, inventory management, grant

writing, instrument maintenance, media and buffer preparation, training graduate and

undergraduate students.

Aurobindo Pharma Ltd, India.

Quality Control Intern Feb, ’10 – Dec, ‘10

Performed quality assurance testing of pharmaceutical drugs (amoxicillin trihydrate) using

physical, chemical and analytical techniques to comply with the FDA regulations.

Working experience with modern instrumentation and techniques like FTIR, Gas

Chromatography, HPCL, Mass Spectrometry and Polarimetery for purity characterization.

Academic Projects

Digital Signal Processing

Used Signal processing algorithms and methods including FIR, FFT,IIR, sampling to analyze

biological- ECG signal.

Implemented MATLAB to design multiple filters (FIR,Butterworth,and Chebyshev) to eliminate

noise from ECG signal.

Statistical Methods in Clinical Trials

Analyzed statistical data for the endpoint (response variable) using Log-Rank test and The Cox

Proportional Hazards models.

Analyzed data with various models available within SAS including ANCOVA and ANOVA analysis

for data from an experiment to reduce dental caries.

Computer Skills

Programming Languages: C, Visual Basic.NET

Modeling Software Auto CAD, Solid Works, MATLAB, SAS and SPSS

Office Tools: MS Office, Excel and PowerPoint and MS Access

Other Adobe Photoshop, ImageJ, GraphPad Prism, Endnote

Professional Memberships

Member of Biomedical engineering society

Member of Society of Neuroscience

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