Quality Control Assistant

RENUKA GANGAKHEDKAR

** ******** ******,

Apartment # 9,

Boston MA 02115

Contact # 617-***-****

Email ID: *************@*****.***

Objective:

Seeking a full time job at an amicable and enterprising organization where I can utilize my research and

organizational skills.

Education:

Graduate education

Professional Science Master, Biotechnology (September 2011 – August 2013)

M.S Biotechnology (Molecular track)

Northeastern University, Boston, Massachusetts

G.P.A 3.3/4

Undergraduate education

Bachelor of Pharmacy (August 2006 – May 2010)

B. Pharmacy

Indira College of Pharmacy, Pune, India

Research Experience:

New England Biolabs, Polymerase and other Enzymes Department, Ipswich, Massachusetts

Research Associate (January 2013 – Present)

• Prepare and purify stearic gate mutants of archeal DNA polymerase D by PCR and Sigma miniprep kit

• Transformation and culturing of archeal proteins in E.coli

• DNA polymerase purification using AKTA system

• Fluorescent assay to check for activity of polymerase and mutants

• Study the kinetics and dynamics of enzymes in real time using single molecule analysis

• Produce proteins using cell free system (PURExpress)

Northeastern University, Center for Drug Discovery, Boston, Massachusetts

Research Assistant (December 2011–August 2012)

• Produced and purified enzymes like Fatty Acid Amide Hydrolase (FAAH) and

MonoacylyglycerolLipase (MGL) from E.coli

• Produced and puried N-acylethanolamine hydrolyzing acid amidase (NAAA) enzyme from

mammalian cells

• Identified inhibitors of FAAH, MGL and NAAA using fluorescent assays

Wockhardt Limited, Aurangabad, India

Intern (June 2009- July 2009)

• Data management of water treatment plant

• Understand the manufacturing process of injectables

S.P. Pharmaceuticals, Jalgaon, India

Intern (May 2009 – June 2009)

• Performed quality control test on batches of injectable

Indira college of Pharmacy, Pune, India

Undergraduate Research projects (September 2009)

• Tested different brands of marketed bottled water using U.S.P and I.P

• “Adaptogenic activity of Ficus glomerata” using swim endurance model on Swiss albino mice

Teaching Experience:

Northeastern University, College of Professional Studies, Boston Massachusetts

Biology Tutor (January 2012 – July 2012)

• Tutored a group of 8 students

Sayali Charitable Trust College of Pharmacy, Aurangabad, India

Professor of Chemistry (September 2010 – May 2011)

• Taught a class of 60 students, conducted labs and graded exams

Skills:

Microbiology:

Bacterial Cell Culturing, Aseptic Techniques, Microscope handling

Analytical Techniques:

PCR, Gel Electrophoresis, Spectroscopy, Fluorescent Assay, SDS-PAGE, Single molecule analysis, nanodrop,

ELISA Western blotting technique.

Protein Purification:

Akta Fast Performance Liquid Chromatography, High Performance Liquid Chromatography, Protein dialysis

Cell Free protein synthesis:

Synthesize archaeal polymerase using PURExpress (cell free E.coli cell extract)

Animal handling:

Dosing and tissue handling of Swiss albino mice, pithing

Computer Skills:

Microsoft Word, Excel, Power Point, Prism, GraphPad, DNAStar and SPSS

Presentation and communication skills

Contact this candidate