Analyst Assistant

Location:

Posted:

October 17, 2013

John R. Pronovost ~ * Lord Road, Chelmsford, Massachusetts 01824, (978) 256-

5810

Email: ***********@*******.***

Objective: To obtain a position in a biotechnology/pharmaceutical company

that will allow for utilization and enhancement of my skills while

providing opportunities for advancement.

Skills and Competencies:

. Trypsinization of attached cells in culture: CHO, WI-38, HELA

. Cell count and viability determination of cells

. Aseptic technique and workplace hygiene

. ELISA assay using Monoclonal antibodies for diagnostic purposes

. Western blot transfer of proteins

. Agar plates prep: streaking of plates

. Positive, negative and differential staining

. pH titration

. Accurate records and reports

. Competent with microscopy

. SDS/PAGE for Protein Analysis of Mammalian Cells

. Molarity and Normality

. Nutrient Media preparation

Education:

12/2012 Middlesex Community College, Lowell, Massachusetts

Biotechnology Certificate

5/1983 University of Massachusetts, Lowell, Massachusetts

Bachelor of Science in Business Administration with a

concentration in Management Information Systems

Experience:

9/2012- Dr. Mariluci Bladon, Middlesex Community College, Lowell,

Massachusetts

12/2012 Lab Assistant Internship ; Tutoring and monitoring the

students during

laboratory sessions

2/2009- ADESA, Boston, Massachusetts

Present Sale Day Auction Driver

11/1998- AQUENT, Inc., Columbia, South Carolina

8/2005 Computer Programmer/Analyst

8/1992- Origin Technologies, Inc., Tampa, Florida

11/1998 Computer Programmer/Analyst

9/1984- ISI Systems, Bedford, Massachusetts

8/1992 Computer Programmer