Quality Control Manufacturing

RUTU GANDHI

** ****** ****** *********, ** ***** Email: ****.********@*****.*** Contact number: 714-***-****

Detailed-oriented and highly motivated bench scientist with 2 years of experience in cell culture, molecular

cloning, sequence analysis tools, cell based assays, fluorescent microscopy, FACS assays and a strong

understanding of mechanisms of drug action including targeted therapies.

PROFESSIONAL EXPERIENCE

Lung Cancer Researcher at Cardinal Bernardin Cancer Center, Maywood, Illinois Dec 2011-Sep 2013

Designed and executed lung cancer experiments for a project aimed at studying epithelial to mesenchymal

transition as a mechanism of drug resistance in non small cell lung cancer

• Maintained and characterized various mammalian cell lines.

• Purified and quantified DNA from bacterial cell lysate and performed PCR for gene amplification

• Performed gene cloning using Gateway cloning technology and tetracycline regulated mammalian gene

expression system

• Performed sequencing of genetically cloned DNA,analyzed sequencing results and generated vector maps

using Vector NTI software

• Generated lung cancer cell lines with overexpression of gene of interest using lentiviral and retroviral

transfection and transduction techniques

• Performed cell based assays including flow cytometric (FACS) assays using BD Canto machine and

subsequent analysis using Flowjo software,MTS/ MTT assay, Crystal violet growth assay.

• Performed complex biochemical/biophysical experiments including SDS-PAGE, Western blot, ELISA for

protein analysis

• Effectively trained junior researcher in research methodology, execution of experiments, data collection and

interpretation

Pharmacy Intern, Bombay Tablets Manufacturing & Co., India May 2010-June 2011

•Provided quality control support in manufacturing operations and

•Prepared batch manufacturing and master formula records

OTHER DEMONSTRATED CAPABILITIES:

Independently designing, executing and troubleshooting experiments

Proficient in biostatistics for biomedical sciences

Efficient in data analysis using Sigma plot, Graph pad Prism, SYSTAT and Excel

Excellent written and oral communication and presentation skills demonstrated during journal clubs and

research in progress seminars

Proficient in executing effective literature search and writing scientific reports

Proficient with computer applications including MS Office and Adobe photoshop

EDUCATION:

MS Molecular Pharmacology and Therapeutics Aug 2011-Sep 2013

Loyola University, Chicago, USA

Thesis: SNAIL induces mesenchymal transition and promotes resistance to EGFR TKIs in Non Small Cell

Lung Cancer harboring EGFR kinase domain mutations.

BS Pharmaceutical Sciences May 2007-May 2011

Gujarat University, India

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