Manager Test
Resume:
Daniel J Kim
***** ******** ** *********@***.***
San Diego, CA 92127
Professional Profile A scientist with expertise in bioassay development with neuroscience background.
Expertise in in-vitro cell based assay development
Expert in immunoassay development
Good with instrumentation and experienced with different detection
methods
Expert in primary cell culture and cancer cell line culture
Expert in imaging analysis using fluorescent dyes
Professional Biossay Development
Accomplishments Introduced multiple primary neuron based bioassay to test and release
numerous recombinant proteins related to neuroscience.
Developed new bioassays and improved existing bioassays for testing
angiogenesis related proteins and antibodies including VEGF family
and Angiopoietin family.
Trained and guided 4 research associates in multidisciplinary projects
to develop bioassays and immunoassays.
The Group under my supervision developed over 100 cell based assays
and immunoassays to test and release over 300 recombinant proteins
related to neuroscience, angiogenesis, adhesion molecules,
developmental biology, and immunology.
Neuroscience
Showed cannabinoids can inhibit new synapse formation in
hippocampal culture system using imaging analysis.
Showed cannabinoids can inhibit neurotransmitter release in
hippocampal culture system using imaging analysis.
Showed synchronized calcium oscillation in hippocampal culture
system using imaging analysis.
Work History September 2007 – August 2013
Manager/Scientist, R&D Systems, Minneapolis, MN
December 2000 – August 2007
Scientist, R&D Systems, Minneapolis, MN
October 1991 – September 1993
Medical Technologist, MedTox Lab, Minneapolis, MN
Education Ph.D. in Pharmacology
U of MN
Minneapolis, MN
September 30, 2000
B.S. in Medical Technology
U of MN, 1991
B.A. in Physics
U of MN, 1989
Partial list of assays Neuronal assays:
Cortical neuron survival assay to test Midkine and Pleotrophin.
developed
DRG neurite outgrowth inhibition assay to test Nogo-A, OMgp and MAG.
Cortical neuron and hippocampal neuron neurite outgrowth assay to neuronal adhesion
molecules and synaptic adhesion molecules .
Substantia nigra dopaminergic neuron survival assay to test GDNF.
Cell-based assays:
HMVEC proliferation assay to test VEGF-C, VEGF-D, and neutralizing antibodies.
EJG cell proliferation assay to test EG-VEGF.
T98G cell proliferation assay to test GAS6.
SHSY5Y cell proliferation assay to test Artemin and GDNF,
TT cell proliferation assay to test Persephin
HUVEC apoptosis assay to test Angiopoietin-1.
Functional ELISA to test:
VEGF-A, -B, -C, -D, and neutralizing antibodies.
VEGF-R1, VEGF-R2, VEGF-R3, and neutralizing antibodies.
Angiopoietin-2, -3, -4, and neutralizing antibodies.
RGM-A, -B, -C, ROBO receptors, and Integrin family.
Other assays:
Calcium flux assay to test Wnt5A.
pSTAT3 Western to test IL-22.
Instruments:
Octet (ForteBio's), NOVOstar (BMG labtech), Gemini (Molecular Devices),
FLx800 (BioTek)
Publications Cannabinoids inhibit the formation of new synapses between hippocampal
neurons in culture. Kim DJ, Thayer SA. Journal of Neuroscience. 2001 May
15;21(10):RC146.
Activation of CB1 cannabinoid receptors inhibits neurotransmitter release from
identified synaptic sites in rat hippocampal cultures. Kim DJ, Thayer SA. Brain
Research. 2000 Jan 10;852(2):398-405.
Neurotoxicity mediated by aberrant patterns of synaptic activity between rat
hippocampal neurons in culture. McLeod JR Jr, Shen M, Kim DJ, Thayer SA.
Journal of Neurophysiology. 1998 Nov;80(5):2688-98.
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