Science/Research

Christopher W. McNown

Home: **** *. ***** **., *******, KS 66007

Phone: 785-***-**** E-Mail: ***********@*****.***

Project Lumen-1820 162nd St., Basehor, KS, 66007

Supervisor: Aaron Krieshok / Telephone: 323-***-****

(08/2011-CURRENT)-Sales Agent/ERP Management

• Making cold calls to potential clients for selling website development projects

• Managing software implementation with multiple systems

• Troubleshooting software integration and website development with clients for finalized projects

• Data entry of website development project inquiries from clients, into data management system, which is

forwarded to website developer

• Implemented dynamic websites utilizing content management systems (Drupal, Wordpress, Joomla)Using

computer-based technologies to manipulate, create, store and retrieve information to express ideas and

communicate with others (Word Processing, Graphics, Multi-Media)

• Using computer-based technologies to locate, access, evaluate, store and retrieve information and to

express ideas and communicate with others (Internet, E-Mail, Spreadsheets and Databases)

• Proficient in Microsoft Office (Excel, Word, Power Point, Outlook, Access, OneNote)

Children’s Mercy Hospital-2401 Gillham Road, Kansas City, MO, 64108

Supervisor: Dr. Tarak Srivastava / Telephone: 816-***-****

(02/2011-08/2011)-RESEARCH ASSISTANT II

• Cell culture techniques with kidney mesangial cells, in addition to podocyte cells, using Saccomanno

fixative

• Green fluorescent protein (GFP), Methyl Violet, Trypan Blue, Rhodamine Phalloidin, DAPI, and AZO

staining for cell viability assays including TUNEL assays.

• SDS-PAGE, Silver Staining, Western Blotting, Immunohistochemistry (IHC) and Protein Concentration

Assays (Coomassie, BCA)

• Flow cytometry Luminex assays (Using Rat Cytokine 10-plex assay)

• Fluid Flow Shear Stress (FFSS) experiments (mechanical stress), to determine the morphological and

cytoskeleton properties (using F-Actin and Crystal Violet Staining), along with protein expression before

and after the FFSS experiments.

• EIA Assays (PGE2 and PGF2α kits) in addition to FGF-23 ELISA assays for clinical testing.

• Clinical assays for the Nephrology lab, which consisted of Citrate, Calcium, Creatinine, Total Protein and

Electrolyte assays.

Oncimmune (CLIA Certified Laboratory)-8960 Commerce Dr. Building #6, DeSoto, KS, 66018

Supervisor: Dr. Laura Peek / Telephone: 913-***-****

(06/2007-02/2011)-RESEARCH TECHNICIAN

• SDS-PAGE, Silver Staining, Western Blotting, ELISA, and Protein Concentration Assays (Coomassie,

BCA) for Quality Control (QC) in a CLIA recognized laboratory.

• Various research techniques including recent work with Dynamic Light Scattering and Fluorescence

Spectroscopy for long-term stability projects with the antigens.

• Organization and management of protein and sample inventory.

• Expertise in laboratory software for data analysis such as Origin 8.0, Prism Graphpad and Microsoft

Office.

KCAS (Formally AaiPharma)-GLP Laboratory-12400 Shawnee Mission Pkwy, Shawnee, KS, 66216

Supervisor: Dr. Gene Ray / Telephone: 913-***-****

(01/2006-05/2007)-ASSISTANT SCIENTIST I

• Worked directly with the weighing of Level 1-5 chemicals, while working in a glove box.

• Bioanalytical drug extractions (solid-phase, liquid-liquid and protein precipitation) focusing on blood,

urine and plasma samples to be analyzed for pharmacokinetic purposes

• Performed radioactive assays, including ELISA and Liquid Scintillation Counting.

• Performed sample analysis with LC/MS and HPLC instrumentation.

• Performed method development, validation, pilot study and the scaled-up assays, as the project manager.

Pharmacology, Toxicology and Therapeutics-KU Med Center- 3901 Rainbow Blvd, Kansas City, KS, 66160

Supervisor: Dr. Sara Li / Telephone: 913-***-****

(08/2005-11/2005)-RESEARCH ASSISTANT

• Pharmacology, Toxicology and Therapeutics research, focusing on Hormonal Cancer research with

Estrogen induced carcinogenesis using ACI rat and Syrian hamster models.

• Preparation of tissues for histopathological analysis (tissue dissection, f ormalin fixation, paraffin

embedding, cryo-sectioning) including the use of a microtome.

• Performed microsurgeries, both survival and non-survival, with estrogen pellet implantation.

• SDS-PAGE, Western Blot, Immunohistochemistry (IHC), Hematoxylin-and-Eosin (H&E) staining,

and Fluorescence Microscopy.

Pharmacology and Toxicology-University of Kansas-1251Wescoe Hall Drive, Lawrence, KS, 66045

Supervisor: Dr. Mary Lou Michaelis / Telephone: 785-***-****

(08/2004-08/2005)-RESEARCH ASSISTANT

• Pharmacology and Toxicology research, focusing on Inhibition of Phosphorylated Tau Protein using a

novel chemical PUGNAc (N-acetylglucosaminidase Inhibitor).

• Western Immuno-blotting, in addition to Caspase-3 Apoptosis Assays, using rat cortical neurons.

• Cell culturing of rat cortical neurons.

• Cell Viability Assays using fluorescence microscopy.

• Exposure to qPCR techniques.

Pharmaceutical Chemistry Department-University of Kansas-2095 Constant Ave., Lawrence, KS, 66047

Supervisor: Dr. C. Russell Middaugh / Telephone: 785-***-****

(02/2003-08/2004)-RESEARCH ASSISTANT

• Pharmaceutical Chemistry research, focusing on Growth Hormone Protein Stabilization using polyanion

excepient screening.

• Pharmaceutical Chemistry research in Endosomal Release of Nanoparticles Assays using specialized

EAhy926 cells.

• Used techniques such as Spectrofluorometry, Circular Dichroism (CD) and Cell Culturing of EAhy926

cells.

• UV Spectrophotometry, Nanoparticle Protein Binding Assays, and Spin Column Gel Filtration.

Publications

• "Interaction of Polyanions with Bovine, Porcine and Human Growth Hormones: Effect on Physical

Stability of these Proteins", Journal of Pharmaceutical Sciences, 17 Aug 2007.

Career History

• 08/2011 CURRENT –Project Lumen

• 02/2011 08/2011 Children’s Mercy Hospital

• 06/2007 02/2011---Oncimmune

• 01/2006 05/2007 KCAS (Formally AaiPharma)

• 08/2005 11/2005 Pharmacology, Toxicology and Therapeutics-KU Med Center

• 08/2004 08/2005 Pharmacology and Toxicology Department-University of Kansas

• 02/2003 08/2004 Pharmaceutical Chemistry Department-University of Kansas

• 08/2000 06/2003 Qualicare Pharmacy-Pharmacy Technician

• 01/1998 08/2000 Dillon’s Pharmacy-Pharmacy Technician

Education

University of Kansas, Lawrence, KS, August 2000-August 2005

B.S. Cell Biology, August 2005

B.G.S. Psychology, August 2005

Minor in Chemistry, August 2005

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