NATHAN F. DUFORD
Salisbury, MA 01952
Cell: (603) 817 - 8350
************@*****.***
SUMMARY OF QUALIFICATIONS
I have eight years' experience in the Biotechnology field and I am skilled
in areas of quality control, process development and laboratory upkeep and
operation.
Relevant Skills
. Proficient with the operation and concepts of Lab-Scale Bioreactors, Cell
Counting and Plating, Media & Buffer Preparation and Centrifugation.
. Have a skill-set beneficial for general bio analytical testing and
assays; e.g. bio burden testing, cell banking, SDS PAGE, ELISA, PCR, etc.
. Proficient in use of Windows OS, MS Office, GraphPad Prism and BioCommand
Plus.
EDUCATION
B.A., Biology, University of Southern Maine, Portland, Maine 1997
New England Biolabs Molecular Biology Summer Workshop, Smith College
June 2013
PROFESSIONAL EXPERIENCE
NEW ENGLAND BIOLABS. Ipswich, MA 2011 - Present (PT)
Production Associate
. Responsible for the setup, maintenance and harvesting of lab scale
bioreactors ranging from 250mL to 15L in a process development
capacity.
. Competent at cell separation via centrifugation with large-scale
SharplesTM batch centrifuge and various scales of Beckman centrifuges.
. Proficient at Media and Buffer preparation for Fermentation use.
. Responsible for all plasmid stability plating, colony counting and
cell count data accumulation.
. Maintains laboratory workspace and performs general upkeep.
. Aids in occasional packaging and labeling of competent cell products.
LONZA Biologics. Portsmouth, NH 1998 - 2003
Quality Control Analyst Level 3
. Routinely tested for Bio Burden, Endotoxins, Coli form, Microbial
identification, Media qualification, Biological Indicators, Final
Product sterility and unknown contaminants, for investigative purposes
of environmental monitoring, water sampling, gas sampling and swab
sampling. Environmental monitoring including air and surface. Reading
EM, in-process, and final product plates and reporting results. Point
person for cell banking.
. Regularly performed glutamine / glutamate testing, pH, conductivity,
SDS PAGE, ELISAs, protein concentration determination, total organic
count testing, gel electrophoresis.
. Responsible for the training and periodic evaluation of new analyst
hires.
. "Above and Beyond" award at Lonza Biologics - granted via nomination
by peers and management.
IDEXX LABS. Westbrook, ME 1997 - 1998
Quality Analyst
. Performed water purity testing, as well as product testing on animal
diagnostic kits, for the purposes of quality control.
ADDITIONAL WORK EXPERIENCE
WINNER'S CIRCLE (family owned restaurant), Salisbury, MA 1994 - Present
(PT)
Manager
. Responsible for overseeing nightly bar and restaurant functions,
staffing, and adhering to state and local regulations.
REFERENCES
Available upon request