Plant Construction

*** * ******** ****, ********, CA *****

YUJUN WANG Ph.D. 626-***-**** ab2s04@r.postjobfree.com

PROFESSIONAL EXPERIENCES:

City of Hope National Medical Center & Beckman Research Institute-Duarte, CA

Research Fellow to Staff Scientist 2005 – Present

My research focuses on the discovery of anticancer drugs and identification of molecular

mechanism of drug action. Discoveries include: 1) Retinoic Acid increased uptake Antisense

Oligonucleotides through endosome pathway; 2) Baicalein upregulates DDIT4 expression

which mediates mTOR inhibition and growth inhibition in cancer cells. Manuscript is ready for

submit.

I have finished one project investigating miRNAs function in breast cancer cells. Discoveries

include: 1) Transforming growth factor-b regulates the sphere-initiating stem cell-like feature

in breast cancer through miRNA-181 and ATM; 2) Context-dependent bidirectional regulation

of the mutS homolog 2 by transforming growth factor β contributes to chemoresistance in

breast cancer cells. Another project focused on molecular mechanism of the incidence of

Marek’s Lymphoma and found: 1) BG1 gene from MHC Loci has a major role in MHC-linked

resistance to malignant lymphoma; 2) Characteristics of BG1 in the incidence of tumor under

the virus infection.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and

Ocean University of China

Research Assistant 2000 – 2004

Molecular mechanism of stress response and resistance in plants. Three novel and important

stress-related genes, OsbHLH1 (GenBank accession number: AY222337), NtTPPL

(Accession number: AY570725), and TaTPC1 (GenBank accession number: AY114121),

were cloned and identified to be involved in plant stress response and resistance.

LAB SKILLS AND TECHNIQUES:

Molecular Biology: DNA and RNA extraction from tissues and cells; PCR; RT-PCR and

Real-time PCR; cDNA RACE; Plasmid construction; DNA homologous recombination;

Southern and Northern blotting; Construction of yeast mutant and functional

complementation analysis; Construction and screening of cDNA library; Yeast or bacteria

two-hybrid; Yeast one-hybrid; Methylation analysis; Transcription factor analysis.

Protein Techniques: Western blotting; Luciferace assay; Expression and purification of

proteins in bacteria; Protein extraction from tissues and cells; Protein Co-Immunoprecipitation;

Protein phosphorylation analysis, and Immunofluorecence staining

Cell and Animal Techniques: Cell culture; Virus (latent and retro) package and amplification,

MTT, Mammosphere-suspension culture; Cell transfection; Drug treatment, Cell separation

(gradient centrifugation, flow cytometry); Stable cell lines generation; Flow cytometry;

Confocal microscope; Preparation of embryonic and kidney fibroblasts as well as splenic cell;

and Animal experimental design and techniques.

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Molecular Biology and Biochemistry Software: DNASTAR, DNAMAN, DNAtools

VectorNTI, SMART, RNAi, shRNA, MicroRNA, and other related tools.

MANUSCRIPT:

1. Yujun Wang, Quanhua Xing, Jin Yan, and John Yim. Baicalein induced cancer cell death

and proliferation retardation by DDIT4-mediated mTOR signaling pathway. (ready for

submit)

PUBLICATIONS:

1. Jinbo Gao, Yujun Wang, Quanhua Xing, Jin Yan, Maheswari Senthil, Yasir Akmal,

Claudia Kowolik, Julia Kang, David Lu, Ming Zhao, Zhixiu Lin, Christopher Cheng,

Richard Yip, and John Yim. Identification of a Natural Compound by Cell-Based

Screening that Enhances Interferon Regulatory Factor-1 Activity and Causes Tumor

Suppression. Mol Cancer Ther. 2011; 10(10): 1774-1783.

2. Y Wang*, Y Yu*, A Tsuyada*, X Ren, X Wu, K Stubblefield, EK Rankin-Gee, and SE Wang.

Transforming growth factor-b regulates the sphere-initiating stem cell-like feature in

breast cancer through miRNA-181 and ATM. Oncogene, 2011; 30(12): 1470-80. (*These

authors contributed equally.)

3. Yang Yu*, Yujun Wang*, Xiubao Ren, Akihiro Tsuyada, Xuejun Li, James Liu, and

Shizhen Emily Wang. Context-dependent bidirectional regulation of the mutS homolog 2

by transforming growth factor β contributes to chemoresistance in breast cancer cells.

Molecular Cancer Research. 2010; 8(12):1633-42. (*The first two authors contributed

equally.)

4. Ronald M. Goto*, Yujun Wang*, Robert L. Taylor, Jr., Patricia S. Wakenell, Kazuyoshi

Hosomichi, Takashi Shiina, Craig Blackmore, W. Elwood Briles, and Marcia M. Miller.

BG1 has a major role in MHC-linked resistance to malignant lymphoma in the chicken.

Proc Natl Acad Sci U S A. 2009; 106(39): 167**-*****. (*Co-first author)

5. Kazuyoshi Hosomichi, Marcia M Miller, Ronald M. Goto, Yujun Wang, Shingo Suzuki,

Jerzy Kulski, Masahide Nishibori, Hidetoshi Inoko, Kei Hanzawa, and Takashi Shiina.

Contribution of mutation, recombination and gene conversion to chicken MHC-B

haplotype diversity. J Immunol, 2008; 181(5): 3393-3399.

6. Yujun Wang, Jianing Yu, Tao Chen, Zhigang Zhang, Yujun Hao, Jinsong Zhang, and

Shouyi Chen. Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat.

Journal of Experiment Botany, 2005; 56(422): 3051-60.

7. Yujun Wang, Yujun Hao, Zhigang Zhang, Tao Chen, Jinsong Zhang, and Shouyi Chen.

Isolation of trehalose-6-phosphate phosphatase gene from tobacco and its functional

analysis in yeast cells. Journal of Plant Physiology, 2005; 162: 215-223.

8. Zhigang Zhang, Hualin Zhou, Tao Chen, Yan Gong, Wanhong Cao, Yujun Wang, Jinsong

Zhang, and Shouyi Chen. Evidence for Serine/Threonine and Histidine kinase activity in

the tobacco ethylene receptor protein NTHK2. Plant Physiology, 2004; 136(2):

2971-2981.

9. Yujun Wang, Yujun Hao, Jixun Dai, Baoxing Du, Jinsong Zhang, and Shouyi Chen.

Improved tolerance of OsbHLH1 transgenic Arabidopsis to low temperature stress. High

Technology Letters, 2004; 14(4): 35-38. (Chinese paper with English Abstract)

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10. Yujun Wang, Zhigang Zhang, Xinjian He, Hualin Zhou, Yuxiang Wen, Jixun Dai, Jinsong

Zhang, and Shouyi Chen. A rice transcription factor OsbHLH1 is involved in cold stress

response. Theoretical and Applied Genetics, 2003; 107(8): 1402-1409.

11. Jianquan Chen, Yi Dong, Yujun Wang, Qiang Liu, Jinsong Zhang, and Shouyi Chen. An

AP2/EREBP-type transcription factor gene from rice is cold inducible and encodes a

nuclear-localized protein. Theoretical and Applied Genetics, 2003; 107(6): 972-979.

12. Zhigang Zhang, Yan Gong, Xinjian He, Yujun Wang, Zhongxu Sun, Jinsong Zhang, and

Shouyi Chen. Cloning of the full-length gene for tobacco ethylene receptor NTHK2 and

characterization of its kinase domain. Acta Botanica Sinica, 2003; 45 (1): 68-72.

13. Zhongxu Sun, Yujun Wang, Jing Liu, Honghua Yang, and Chunzhi Zhao. A new filter

bridge rooting method for plant microprogation. Journal of Shandong Agricultural

University, 2002; 33(3): 257-263. (Chinese paper with English Abstract)

14. Zhongxu Sun, Jing Liu, Yujun Wang, Yanyi Zhang, and Zhilin Qiou. Research on out

bottle rooting technique of fruit seedling in vitro with filter-paper bridge. Acta Horticulturae

Sinica, 2001; 28 (4): 345-347. (Chinese paper with English Abstract)

15. Zhongxu Sun, Jing Liu, Yujun Wang, Deping Yang, and Jie Liu. Studies on tissue culture

and fertility of the improved tea cultivars in Shandong Province. Journal of Tea Science,

2000; 20(2): 125-129. (Chinese paper with English Abstract)

ABSTRACTS AND PRESENTATIONS:

1. Akmal Y, Senthil M, Yan J, Xing Q, Wang Y, Tully D, Yim J. Combination of Natural

Product (Baicalein) and Cisplatin Suppresses Tumor Growth and Induces Apoptosis in

Mouse Breast Cancer. J Am Coll Surg. 2011;213(3), Suppl, p. S136

2. Y Akmal, M Senthil, J Yan, Q Xing, Y Wang, G Somlo, J Yim. Combination of a Natural

Compound (Baicalein) and Paclitaxel Result in Synergistic Apoptosis in Mouse Breast

Cancer Cells. J Surg Research 2011 Feb; 165(2): 218 (21.7).

3. Y. Akmal, M. Senthil, Q. Xing, J.Yan, Y.Wang, J.Yim. Extract From Traditional Chinese

Herb Scutellaria Baicalensis Suppresses Tumors and Inhibits p-Akt in Mice. Ann Surg

Onc 2011 Feb; 18(Suppl 1):S845, P24.

4. Yasir Mohammad Akmal, Maheswari Senthil, Quanhua Xing, Jin Yan, Yujun Wang, and

John Yim. Effect of an extract from the traditional Chinese herb Scutellaria baicalensis on

tumor growth and p-Akt in mice. (2010) 2010 Breast Cancer Symposium, Suburban

Washington, DC.

5. Yang Yu, Yujun Wang, Akihiro Tsuyada, and Shizhen Emily Wang. Deregulation of

MicroRNAs by Transforming Growth Factor β in Breast Cancer Cells. (2010) 2010 Annual

poster session, Duarte, CA.

6. Ronald M. Goto, Ali Ehsani, Yujun Wang, and Marcia M. Miller. MicroRNA and BG1 in

MHC-linked resistance to Marek’s disease tumors. (2010) The 5th International Workshop

on the Molecular Pathogenesis of Marek's Disease Virus & 1st Symposium on Avian

Herpesviruses. Athens, GA 30602. United States

7. Yujun Wang, Ronald M. Goto Patricia S. Wakenell, Robert L. Taylor. Jr, and Marcia M.

Miller. Genetic Resistance to GaHV-2 induced lymphoma in the Chicken Model. (2008)

11th International Conference on Malignancies in AIDS and Other Acquired

Immunodeficiencies (ICMADI). Bethesda, Maryland.

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8. Yujun Wang, Ronald M. Goto, and Marcia M. Miller. BG1, a candidate gene affecting the

occurrence of the herpesvirus-associated avian lymphomas. (2008) Experimental Biology

2008 (AAA, AAI, APS, ASBMB, ASIP, ASN, and ASPET) San Diego, California.

9. Marcia M. Miller, Yujun Wang, Lei Zhang, Ronald M. Goto, Mary E. Delany, Charmaine M.

Robinson, W. Elwood Briles, Herry D. Hunt, Takashi Shiina, Kazuyoshi Hosomichi,

Kazuyo Yanagiya, Sayoko Shimizu, and Hidetoshi Inoko. Genes, variability, and linkage

in the chicken MHC B and Y region. (2008) National Research Initiative (NRI) Annual

Investigator Meeting, San Diego, California.

10. Yujun Wang, Ronald M. Goto, and Marcia M. Miller. BG1, a candidate locus in the MHC

affecting virally-induced tumors. (2007) 27th Annual Advance of the Research Staff

Organization. Lake Arrowhead, California.

11. Yujun Wang, Ronald M. Goto, Patricia S. Wakenell, Robert L. Taylor. Jr, and Marcia M.

Miller. Expression, structure localization, and allelic variability of the BG1 locus in the

chicken Major Histocopatibility Complex. (2006) 2006 Workshop on Chicken Genomics &

Development. Cold Spring Harbor, New York.

12. Marcia M. Miller, Patricia S. Wakenell, Ronald M. Goto, Yujun Wang, and Robert L. Taylor.

Jr. MHC genetics in the suppression of the tumors caused by a highly oncogenic avian

herpesvirus. (2006) Annual Meeting of the American Association of the Immunologist.

Boston, Massachusettes.

13. Marcia M. Miller, Ronald M. Goto, Yujun Wang, Patricia S. Wakenell, and Robert L. Taylor.

Jr. Genetics of tumors suppression in the avian Marek’s Disease model. (2005) 9th

International Conference on the Malignancies in AIDS and other Acquired

Immunodeficiencies. North Bethesda, Maryland.

EDUCATION:

Ocean University of China and Institute of Genetics and Developmental Biology,

Chinese Academy of Sciences, P.R. China. (Joint) 2003

Ph. D. in Molecular Biology

Shandong Agricultural University, Tai’an, P.R. China 2000

M.S. in Biology

Shandong Agricultural University, Tai’an, P.R. China 1997

B.S. in Biology

PATENT:

Shouyi Chen, Jinsong Zhang, Yujun Wang, Zhigang Zhang, Xinjian He, and Hualin Zhou. A

rice transcription factor associated with the tolerance to low temperature stress, its coding

gene and application. CN03123913.7, P.R. China State Intellectual Property Office; May 20,

2003.

HONORS AND AWARDS:

1. Science and Technology Advancement Award by Shandong Provincial government, P.R.

China, 2002.

2. Excellent Graduate Student, Shandong Agricultural University, P.R. China, 2000.

3. Science and Technology Advancement Award by Shandong Provincial government, P.R.

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China, 1999.

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