Resume
Resume:
A NNETTE MARIE MEDINA
** ********* **. *** *, Cambridge, MA, 02137 787-***-**** ab2ewl@r.postjobfree.com
E DUCATION
University of California, San Diego. USA July 2013
Ph.D., Inorganic Chemistry
Thesis: Rational Design and Evolution of Supramolecular Metal-Binding Protein Assemblies
GPA: 3.6
Supervised by Prof. F. Akif Tezcan
University of Puerto Rico, Rio Piedras. Puerto Rico, USA June 2007
B.S., Chemistry
GPA: 3.6
Honors thesis: Intercalation of Iron Complexes into Zirconium Phosphate Frameworks
Supervised by: Prof. Jorge Colon
A WARDS
Graduate:
Carl Storm Underrepresented Minority (CSURM) Fellowship January 2012
Cellular and Molecular Genetics Training Grant (NIH) June 2009 – June 2012
San Diego Fellowship for Graduate Studies September 2007 – June 2009
Alliances for Graduate Education and the Professoriate (AGEP-NSF) Graduate Fellowship Summer 2007
Undergraduate:
Minority Access for Research Careers (MARC-NIH) Undergraduate Fellowship September 2005 – May 2007
Outstanding poster at Annual Biomedical Research Conference for Minority Students (ABRCMS) Fall 2006
Puerto Rico Louis Stokes Alliance for Minority Participation (PR-LSAMP-NSF) Fellowship June 2010 – June 2012
P EER-REVIEWED PUBLICATIONS
Covalent modification of a metal-organic framework with isocyanates: probing substrate scope and reactivity. E Dugan, Z Wang, M
Okamura, A Medina, SM Cohen. Chemical Communications (cover article), 2008, 3366.
Evolution of metal selectivity in templated protein interfaces. JD Brodin, A Medina-Morales, T Ni, EN Salgado, XI Ambroggio, FA
Tezcan. Journal of the American Chemical Society. 132 (25), 2010, pp 8610-8617.
In vitro and in vivo self-assembly of a protein cryptand via templated disulfide bonds. A Medina-Morales, Alfredo Perez, JD Brodin,
FA Tezcan. Journal of the American Chemical Society. Accepted July 19, 2013.
Metal-templated design of supramolecular protein assemblies. A Medina-Morales, Alfredo Perez, FA Tezcan. (Under review).
Inorganic Chemistry.
Unsaturated reactive copper centers within engineered protein scaffolds. A Medina-Morales, Alfredo Perez, FA Tezcan. (In
preparation).
R ESEARCH EXPERIENCE
Graduate Research in Bioinorganic Chemistry- Tezcan Laboratory, UCSD, La Jolla, CA Fall 2007-July 2013
• Design of supramolecular protein scaffolds with metal-binding functionalities
• Techniques and instrumentation
Site-directed mutagenesis
o
Recombinant protein expression with Escherichia coli
o
Protein purification techniques: Ion exchange chromatography, size exclusion chromatography (SEC), Fast Protein
o
Liquid Chromatography (FPLC)
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
o
High Pressure Liquid Chromatography (HPLC)
o
Analytical ultracentrifugation and sedimentation velocity analysis for biophysical characterization of protein complexes
o
Protein crystallization techniques: sitting-drop, hanging-drop, seeding, anaerobic crystals
o
A NNETTE MARIE MEDINA PAGE 2
Collection of diffraction data for protein crystals: Remote-access to Stanford Synchrotron Research Laboratory
o
(SSRL); In-house use of Bruker Proteum diffractometer
Protein crystallography data analysis and refinement: Mosflm, Scala, CCP4 (Refmac, Phaser, Molrep), Coot
o
Spectroscopic techniques
o
Circular Dichroism (CD) spectroscopy
UV-Vis absorption spectrophotometry
Fluorescence UV-Vis spectrophotometry
Electron Paramagnetic Resonance spectroscopy (EPR) of metalloproteins
Matrix-assisted laser desorption/ionization spectroscopy (MALDI)
Work with anaerobic glove box and Schlenk line technique
o
Isothermal Titration Calorimetry (ITC) for thermodynamic analysis
o
Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-OES) for metal identification and quantification
o
Software: Proficient with Microsoft Office (Word, Excel, PowerPoint), Matlab, OriginLab
o
Summer Rotation in Inorganic Chemistry- Cohen Laboratory, UCSD, La Jolla, CA Summer 2007
• Postsynthetic modification of Metal-Organic Frameworks (MOF’s)
• Techniques and instrumentation
Inorganic synthesis of MOF’s
o
1
H Nuclear Magnetic Resonance
o
Electrospray ionization mass spectrometry
o
Thermal gravimetric analysis
o
Undergraduate Research- Turro Laboratory, Columbia University, New York, NY Summer 2006
• Photophysical characterization of Ru(phen)2dppz2+ within supramolecular micellar environments containing organic fluorophores
• Techniques and instrumentation
1
H Nuclear Magnetic Resonance
o
Fluorescence spectrophotometry: Fluorescence Resonance Energy Transfer (FRET) technique
o
Undergraduate Research- Colon Laboratory, UPR, San Juan, Puerto Rico, USA 2005-2007
• Intercalation of iron polypyridyl complexes into zirconium phosphate frameworks
• Techniques and instrumentation
Powder X-Ray diffraction
o
Inorganic synthesis
o
Fluorescence spectrophotometry
o
UV-Vis spectrophotometry
o
P OSTERS AND PRESENTATIONS
Talk: Metal-templated design of protein macrocycles Spring 2012
American Chemical Society National Meeting- San Diego, CA, USA
Talk: Metal-templated design of protein macrocycles with metal-based chemical functionalities Spring 2012
Gordon Research Seminar on Bioinorganic Chemistry- Ventura, CA, USA
Poster: Metal-templated design of protein macrocycles with metal-based chemical functionalities Spring 2012
Gordon Research Conference on Metals in Biology- Ventura, CA, USA
Poster: Protein self-assembly controlled by coordination chemistry Spring 2010
American Chemical Society National Meeting- San Francisco, CA, USA
Poster: Protein self-assembly controlled by coordination chemistry Summer 2008
American Chemical Society National Meeting- Philadelphia, PA, USA
Talk: Intercalation of Iron Polypyridyls into Zirconium Phosphate Frameworks Spring 2007
American Chemical Society Junior Technical Meeting- Cayey, Puerto Rico, USA
Poster: Intercalation of fullerene into β -Cyclodextrin and into Layered Double Hydroxides Fall 2006
American Chemical Society National Meeting- San Francisco, CA, USA
Talk: Photophysical characterization of Ru(phen)2dppz2+ within supramolecular micellar environments Fall 2006
Annual Biomedical Research Conference for Minority Students- Anaheim, CA, USA
T EACHING EXPERIENCE
A NNETTE MARIE MEDINA PAGE 3
Teaching Assistant- General Chemistry (CHEM6A) Fall 2007
Developed tri-weekly hourly lectures and review sessions, graded exams and prepared quizzes
Teaching Assistant- Analytical Chemistry Laboratory (CHEM100A) Winter 2008
Prepared quizzes, evaluated laboratory reports, supervised students, prepared weekly lectures
Teaching Assistant- Organic Chemistry Laboratory I (CHEM143A) Winter 2008
Prepared quizzes, evaluated laboratory reports, supervised students, prepared weekly lectures
M ENTORING AND VOLUNTEERING
Mentor for undergraduate researchers: mentored 3 undergraduate students on a one-to-one basis through Ph.D. research.
Participated in science outreach opportunities with Women in Science organization.
M EMBERSHIPS
American Chemical Society- member since 2008.
SACNAS member since 2012.
L ANGUAGES
Spanish- native speaker
English- native speaker
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