JAYAPRIYA JAYARAMAN **** Costa Verde Blvd, Apt ***7, San Diego CA-92122

E-mail: ab0wlc@r.postjobfree.com Phone: 518-***-****

Dynamic and competent individual with versatile skill sets in molecular biology, protein characterization, process scale-up and

technology transfer. Strong fundamental knowledge of protein chemistry, biochemical/chemical engineering. Very proficient in

molecular cloning, vector construction and PCR. Nearly two years of experience with antibody engineering and design.Very

adaptable and quick learner with proven experience as an independent researcher as well as a collaborative team player.

PROFESSIONAL PROFILE: http://www.linkedin.com/pub/jayapriya-jayaraman/15/507/7ab

SKILLS

Bioanalytical characterization methods: Dynamic Light Scattering, Circular Dichroism, Tryptophan Fluorescence, SDS-PAGE

Molecular Biology: PCR, Molecular cloning and vector construction, Gene synthesis

Protein expression and purification: Recombinant protein expression in E.Coli, Purification of affinity tagged proteins

Familiar with immunological assays such as Western Blotting, ELISA and FACS

Familiar with high-throughput cloning

EXPERIENCE

Part-time Research Assistant, Antibody Engineering/Design, April 2013-Present

Fabrus Functional Antibodies Inc, San Diego-CA

● Design and development of antibody vector constructs

● Conducting PCR, molecular cloning and gene synthesis experiments

Volunteer Teaching Assistant, Applied Biotechnology, April 2013-May 2013

San Diego Miramar College, San Diego-CA

● Hands on experience in mammalian cell culture practices

● Assisting in training a heterogeneous group of students on PCR, restriction digests, DNA isolation and

molecular cloning

Graduate Research Assistant, January 2011- December 2012

Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy -NY

Advisor: Dr. Peter M Tessier, Assistant Professor, Rensselaer Polytechnic Institute, Troy, NY

High-throughput analysis of antibody self-interactions

● Developed high-throughput gold nanoparticle assay to study antibody colloidal stability in various

formulation conditions

● Characterized gold nanoparticle-antibody conjugate interactions using surface plasmon resonance

● Elucidated diffusion interaction parameter of antibodies as a function of pH and ionic strength using

dynamic light scattering

Design of novel antibodies targeting mis-folded proteins

● Developed antigen binding fragments to target various conformers of amyloidogenic proteins

● Developed and optimized linker sequence for bivalent linker connected single domain antibodies targeting

amyloidogenic proteins

● Cloned, expressed and purified various antibody formats

● Characterized secondary structure and refolding of antibody formats using circular dichroism

Junior Manager, Integrated Product Development, July 2008-June 2010

Dr. Reddy’s Laboratories, Hyderabad-India

● Scale up of engineering unit operations such as crystallization, drying, filtration and reactions

● Equipment selection and sizing for different manufacturing operations

● Cross-functional co-ordination with different teams to ensure effective technology transfer

● Troubleshooting technical issues in plant operations during scale up and validation batches

● Applied process modifications during lab development to ensure scalable and robust processes

EDUCATION

M S Chemical Engineering (emphasis on Biological Engineering), 2012, Rensselaer Polytechnic Institute, Troy,NY

M S Chemical Engineering, 2008, Indian Institute of Technology, Madras, India

B S Chemical Engineering, 2006, Anna University, Chennai, India

REFERENCES

1. Dr. Peter Tessier, Assistant Professor

Dept. of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY

E-mail: ab0wlc@r.postjobfree.com

2. Dr. Omar Bazirgan, Senior Scientist

Fabrus Functional Antibodies LLC, San Diego, CA

E-mail: ab0wlc@r.postjobfree.com

3. Dr. Miguel de los Rios, Vice President of R&D

Fabrus Functional Antibodies LLC, San Diego, CA

E-mail: ab0wlc@r.postjobfree.com

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