Training C

Curriculum Vitae

Personal information

First name(s) / Surname(s) Juan José Jiménez Martínez

Address(es) 1, Federico Moreno Torroba. 28007 Madrid. Spain

Telephone(s) +34-915****** Mobile: +34-639******

E-mail ******.***************@*****.**

Nationality, Date of birth, Gender Spanish, 10/06/1979, Male

Work experience

Dates September 2007, initiation of the doctoral studies in Veterinary Sciences

Occupation or position held PhD Student (Thesis defence December 2012)

Main activities and responsibilities Development and use of genetic tools for cloning, production and functional expression of natural antimicrobial peptides (bacteriocins) by heterologous lactic acid bacteria (LAB) and yeasts. Generation of rabbit polyclonal antibodies specific for bacteriocins and development of inmuno-chemical assays (NCI-ELISA) to determine the production of bacteriocins in the supernatants of the producer LAB and yeasts. Sequencing of bacteriocins cluster in Enterococcus faecalis.

Name and address of employer University Complutense of Madrid (UCM). Avenida Puerta de Hierro s/n. 28040 Madrid. Spain.

Type of business or sector Research and Education

Dates September 2005 – July 2007

Occupation or position held Postgraduate student

Main activities and responsibilities Genotoxicity of N-nitrosocompounds and evaluation of the relationship structure-genotoxicity.

Name and address of employer University of Salamanca (USAL). Plaza Patio de Escuelas Menores, 1. 37007 Salamanca. Spain.

Type of business or sector Research and Education

Dates September 2004 – December 2004

Occupation or position held Postgraduate student

Main activities and responsibilities Maintenance of Food-and-Mouth Disease (FMD) virus for vaccine developments.

Name and address of employer Center for Molecular Biology “Severo Ochoa” (CBMSO). University Autónoma of Madrid (UAM). C/ Nicolas Cabrera, 1. Campus Cantoblanco. 28049 Madrid. Spain.

Type of business or sector Research and Education

Education and training

Dates June 2006

Title of qualification awarded Diploma for Advanced Studies (DEA) in Physical-Chemistry

Principal subjects/occupational skills covered Nitrosation of phenolic compounds and some halogenated phenol derivatives

Name and type of organisation providing education and training Department of Physical Chemistry. University of Salamanca (USAL). Plaza de los Caidos, s/n. 37007 Salamanca. Spain.

Dates June 2004

Title of qualification awarded Certificate of Pedagogical Aptitude (CPA)

Principal subjects/occupational skills covered Allowance for teaching all around Europe

Name and type of organisation providing education and training Faculty of Education, University Complutense of Madrid, C/ Rector Royo Villanova s/n. Ciudad Universitaria. 28040 Madrid, Spain

Dates September 2003

Title of qualification awarded Bachelor degree (BSc.) in Biology

Principal subjects/occupational skills covered Major in Molecular Biology

Name and type of organisation providing education and training SEK University. Campus Santa Cruz la Real. C/ Cardenal Zúñiga, 12. 40003 Segovia. Spain.

Personal skills and competences

Mother tongue(s) Spanish

Other language(s)

Self-assessment Understanding Speaking Writing

European level Listening Reading Spoken interaction Spoken production

English C1 Proficience user C1 Proficience user C1 Proficience user C1 Proficience user C1 Proficience user

Norwegian A1 Basic user A1 Basic user A1 Basic user A1 Basic user A1 Basic user Common European Framework of Reference for Languages

Social skills and competences - Good working skills gained through diverse studies in different universities

- Ability to adapt to multicultural environments

- Ability to perform research in group

Organisational skills and competences - Organization Member (Secretary) of the “XII Reunión Científica de la Sociedad Española de Mutagénesis Ambiental”, SEK University. July, 2004. Segovia, Spain.

Technical skills and competences - Molecular biology methods

- Genetic engineering applications and methods

- Bacteria and yeast molecular biology

- Inmunochemical methods

- Mutagenesis and genotoxicity

- Protein purification methods

- Pathogens Microbiology (Salmonella, Campylobacter, Listeria)

Computer skills and competences - Microsoft office (Word, Excel, Powerpoint, etc.)

- Bioinformatic tools (NCBI blast, Expasy, BAGEL, etc.)

Additional information List of relevant publications

Jiménez J.J., J. Borrero, L. Gútiez, C. Herranz, L.M. Cintas, and P.E. Hernández. Production and functional expression of the bacteriocin sakacin A and two genetically-derived chimeras by different lactic acid bacteria (LAB) and the yeasts Pichia pastoris and Kluyveromyces lactis. (Manuscript in preparation).

Jiménez J.J., J. Borrero, L. Gútiez, S. Arbulu, C. Herranz, L.M. Cintas, and P.E. Hernández. Use of synthetic genes for production and functional expression of the bacteriocins, enterocin A and bacteriocin E50-52, by Pichia pastoris and Kluyveromyces lactis. (Manuscript in preparation).

Jiménez J.J., D.B. Diep, J. Borrero, L. Gútiez, C. Herranz, I.F. Nes, L.M. Cintas, and P.E. Hernández. Evaluation of different protein expression vectors for the cloning, production, and functional expression of the bacteriocin enterocin A by Lactobacillus spp. (Manuscript in preparation).

Jiménez J.J., J. Borrero, J. Sánchez, D. Diep, C. Herranz, I.F Nes, L.M Cintas, and P.E. Hernández. Enterococcus faecalis DBH18, isolated from Mallard ducks (Anas platyrhynchos), encodes the bacteriocin-producing genes ef1097, entL50A-entL50B, and the entL50A-entL50B-like genes termed entJSA and entJSB. (Manuscript in preparation).

Borrero, J., G. Kunze, J.J. Jiménez, E. Böer, L. Gútiez, C. Herranz, L.M. Cintas, and P.E. Hernández. Cloning, production and functional expression of the bacteriocin enterocin A, produced by Enterococcus faecium T136, by the yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha and Arxula adeninivorans. Applied and Environmental Microbiology, doi: 10.1128/AEM.00530-12

Borrero, J., J.J. Jiménez, L. Gútiez, C. Herranz, L.M. Cintas, and P.E. Hernández (2011). Use of the usp45 lactococcal secretion signal sequence to drive the secretion and functional expresión of enterecoccal bacteriocins in Lactococcus lactis. Applied Microbiology and Biotechnology, 89: 131-143.

Borrero, J., J.J. Jiménez, L. Gútiez, C. Herranz, L.M Cintas, and P.E. Hernández (2011). Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A (EntA) in lactic acid bacteria. Journal of Biotechnology, 156: 76-86.

List of relevant communications/posters to congresses

• Jiménez J.J., J. Borrero, L. Gútiez, S. Arbulu, C. Herranz and P. E. Hernández. (2012) Cloning, production and functional expression of the bacteriocin sakacin A, and two of its genetically-derived chimeras, by lactoc acid bacteria (LAB) and the yeasts Pichia pastoris and Kluyveromyces lactis. 3rd International Symposium on Antimicrobial Peptides. Abstracts, C-51. Lille, France, 13-15 of June.

• Jiménez J.J., J. Borrero, L. Gútiez, S. Arbulu, C. Herranz and P. E. Hernández. (2011). Cloning Cloning and functional expression of an enterocin A (EntA) synthetic gene in Pichia pastoris and Kluyveromyces lactis. VI Jornadas Complutenses, V Congreso Nacional de Investigación para alumnos de pregrado en Ciencias de la Salud y X Congreso de Ciencias Veterinarias y Biomédicas. Reduca (Recursos Educativos). Serie Congresos Alumnos, 3 (3): 51-52. Madrid, Spain, 7 y 8 of April.

• Jiménez, J.J., J. Borrero, L. Gútiez, E. Muñoz, C. Campanero, B. Gómez-Sala, L. M. Cintas and P. E. Hernández. (2010). Production and functional expression of sakacin A (SakA) and two of its chimeras in two systems of heterologous production developed in lactic acid bacteria (LAB) and in the yeasts Pichia pastoris and Kluyveromyces lactis. V Jornadas Complutenses-IV Congreso Nacional de Investigación para Alumnos de Pregrado en Ciencias de la Salud-IX Congreso de Ciencias Veterinarias y Biomédicas. Madrid, Spain, 22-24 de April.

• Jiménez, J.J., J. Borrero, L. Gútiez, L.M. Cintas and P.E. Hernández. (2009).Sakacin A (SakA) chimeras, produced by Lactobacillus sakei Lb706, fused to diverse signal peptides (SP) in adequated expression vectors, allow thier cloning, production and functional expression by other lactic acid bacteria (LAB). VIII Congreso de Ciencias Veterinarias y Biomédicas. Comunicaciones. Madrid, Spain, 27-29 April. (Best Oral Exposition Award).

• Jiménez, J.J., J. Borrero, L. Gútiez, L.M. Cintas and P.E. Hernández (2008). Cloning and heterologous production of sakacin A (SakA) from Lactobacillus sakei Lb 706 in Lactococcus lactis using a nisine-inducible expression vector. VII Congreso de Ciencias Veterinarias y Biomédicas. Comunicaciones, p. 80. Madrid, Spain, 28-30 April.

• Jiménez, J.J., F. Bartolomé, C. Ruíz-Roldán and S. González-Mancebo (2004). Genotoxicity of C-nitrosocompounds: structure-genotoxicity relation. XIII Reunión Científica de la Sociedad Española de Mutagénesis Ambiental. Comunicaciones, p.21. Segovia, Spain, 7-9 July.

• Jiménez, J.J., F. Bartolomé, R. Matesanz, E. Ruano, C. Ruiz, A. Martín and S. González-Mancebo (2003). Genotoxicity evaluation of the nitrosocompounds formed with hydroquinonic antioxidants through SOS Chromotest. XII Reunión Científica de la Sociedad Española de Mutagénesis Ambiental. Comuniaciones, p.15. Santiago de Compostela, Spain, 2-4 July.

International research center stays

Center: Laboratory of Microbial Gene Technology and Food Microbiology. Department of Chemistry, Biotechnology and Food Science. Norwegian University of Life Sciences. Ås, Norway.

Periods: 12th of April, 2010 - 5th of September, 2010 and 11th of February, 2011 - 6th of July, 2011.

Main Supervisor: Prof. Ingolf F. Nes

Teaching assistant in the subjects:

- “Hygiene, Inspection and Food Control” (Grade in Veterinary Medicine, UCM)

- “Food Hygiene” (Grade in Food Science and Technology, UCM)

- “Food Safety” (Grade in Human Nutrition and Dietetics, UCM)

- “Microbiology” (Grade in Biology, UAM)

- “Toxicology” (Grade in Biology, SEK University)

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