Research Specialist II

Salonee Parikh

** ** ********* ******, #****, Boston MA 02120

301-***-**** **************@*****.***

Professional Experience

Bioanalytics Department, Shire- Human Genetic Therapies, Lexington, MA August 2011- Present

Research Specialist II

• Develop assays to determine the biomarkers for Hunter’s and San Fillipo diseases.

• Involved in performing enzyme assays for particular biomarkers indicated in disease in a cGMP and GLP environment using CSF samples.

• Absorbance measurements using Spectramax and data interpretation using Microsoft Excel.

• QA, validation and documentation of results obtained from experiments.

Laboratory for Drug Discovery in Neurodegeneration, Brigham and Women’s Hospital, Cambridge, MA

Research Volunteer January-April 2011

• Maintaining mammalian cell lines (HEK 293, HEK 293- FT, HEK 293 with FlpIn systems), media preparation, freezing back cells, plating cells in 96 and 384 well-microplate using a multidrop, cell counting using Invitrogen cell countess and transient cell transfections using lipofectamine

• Responsible for DNA prep, cell lysate collection, BCA protein assay, western blot, ELISA.

• Experience with optimization and screening of compounds and development of cell-based assays for the purpose of siRNA screening and high-throughput screening (HTS).

Division of Sleep Medicine, Brigham and Women’s Hospital, Boston, MA

Research Assistant May 2010-April 2011

• Assisted in various sleep studies pertaining to measurement of upper airway collapsibility during obstructive sleep apnea with experience in patient interaction, data collection and extensive literature reviews.

• Data analysis using SPIKE 2, MS DOS, Microsoft Excel and SPSS for projects involving the determination of the upper airway using acoustic pharyngometry and effect of sleep deprivation on genioglossus activity.

Dr. Loring’s Laboratory, Northeastern University, Boston, MA

Research Assistant January- July 2010

• Cell line maintenance (SHEP-1 and SH-SY5Y cells), cell transfections, radioactive binding assays.

• To determine the anti inflammatory activity of nicotine through the alpha 7 nicotinic receptors using SH-SY5Y human neuroblastoma cells which bind alpha bungarotoxin.

• To establish killing concentrations of antibiotics like puromycin, geneticin (G418) and zeocin using SHEP 1 cells and SH-SY5Y cells (cellular viability assays).

Pharmaceutical Sciences Laboratory, Northeastern University, Boston, MA September-December 2010

• Hands-on experience with PCR, western blotting, ELISA, RNA extraction, cDNA synthesis (PCR), cell-lysate collection, BCA assay, nanoparticles preparation, restriction digestion of DNA, plasmid preparation, cytotoxicity assay, cell culturing (NIH3T3), cell transfection, affinity chromatography, enzyme kinetics and UV spectroscopy, gel electrophoresis.

Skills

In-vitro: UV spectroscopy, chromatography, nanodrop, mammalian cell culturing, radioactive binding assays, BCA assay, cell transfection, luminescence and fluorescent plate readers, plasmid preparation, PCR, ELISA, western blot, DNA prep, RNA extraction, DNA purification, gel electrophoreis, microscopy, enzymatic assays, SpectraMax, fluorescence microscopy.

In-vivo: Oral, IV,SC and IP dosing of small laboratory animals including rats and mice, animal husbandry, observing animals’ behavior in response to the administration of the test drugs.

Computer: Chemsketch, Spike 2, EndNote, GraphPad Prism, MS Word, Excel, Powerpoint, Outlook, SoftMax Pro.

Education

Northeastern University Boston, MA

Master of Pharmaceutical Sciences-Pharmacology May 2011 (GPA 3.78/4.0)

Nirma Institute of Pharmacy Ahmedabad, India

Bachelor of Pharmacy (B. Pharm) April 2009 (GPA 8.93/10)

Thesis: “Novel Drug Delivery System for Cancer Chemotherapy”

Published Abstracts

• Parikh S, White DP, Jordan AS, Merchia P, Malhotra A, Eckert DJ. Sleep deprivation impairs genioglossus muscle responsiveness. Am J Respir Crit Care Med 2011.

• Parikh S, White DP, Jordan AS, Merchia P, Malhotra A, Eckert DJ. 36 hours of sleep deprivation reduces genioglossus muscle activity during hypercapnia and inspiratory resistive loads during wakefulness. Sleep 2011;34:A153.

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