Medical Clinical Laboratory

CURRICULUM VITAE

PERSONAL INFORMATION:

Full name: Daniele Baldi

Date of birth: September 16th 1981

Place of birth: Arezzo, Italy

Address: via delle case 64 Arezzo 52041 ITALY

** ****rside drive 10024 11R

Telephones: +39-575-****** home phone

(+39) 348- 97 59 688 cell phone

E-mail: ********@*******.**

Nationality: Italian

Gender: male

EDUCATION AND TRAINING:

10/2007-07/2011, UNIVERSITY OF FLORENCE, GRADUATE SCHOOL OF MEDICINE, MS degree with honors, in medical biotechnology , molecular oncology and pathology curricula, with the Italian degree score of i 110/110 cum laude.

Master degree qualification: medical laboratory scientist and doctor in medical biotechnologies

• 06/2009-07/2011 Thesis, Research scientist internship

Project title: The role of hipoxia and the HDAC inhibitor, VPA, as epigenetic modulators in APL

Human cell line NB4.

• I worked, for 2 years, on my research-based thesis carried out in the department of experimental and molecular pathology and oncology .This project was submitted as final examination of the MS degree in Medical Biotechnology.

• I evaluated the VPA and HIPOXIA effects on viability, histones acetylation, cell death and cell cycle.

• Human NB4 cell line (M3 acute myeloid leukemia cell line) cultures were initiated, cultured, maintained at 0.3% 02(hypoxia) and/or 20% O2 (normoxia), with or without pharmacological stimulation using VPA in different concentrations (untreated, 0.5, 1 mM).

• Cells were stained and counted with an optical microscope, lysed for western blotting assays, subjected to annexin-V and PI test and cell cycle distribution using flow cytofluorimetric assays.

• 12/2009, I took part in the 3 days of national meeting, the cell death and its physio-pathological and therapeutic significance, organized by Italian cell cultures association and stem cell foundation for human life in collaboration with University of Florence .

09/2000-11/2004 UNIVERSITY OF SIENA, SCHOOL OF MEDICINE, BS degree in medical laboratory medicine with the Italian degree score of 110/110 cum laude.

Bachelor Degree qualification: doctor in biomedical and clinical laboratory technologies, clinical medicine technologist

• Clinical and laboratory internship and traineeship, I worked for 4 years, about 1200 hours, on the professional practical training and traineeship in several diagnostic areas and clinical laboratories, 1 to 4 months in each laboratory.

• Executed and analyzed tests, of both routine and special laboratory assays of patient specimens, in many areas including cytogenetic, biochemistry, hematology, urinalysis, sierology, microbiology, pathological anatomy unit, blood bank and Trasfusional medicine unit and others to aid physicians in diagnosing and treating diseases.

• I have performed several types of diagnostic and clinical tests, manual and also automated, operated and calibrated an assortment of laboratory testing equipment and evaluated quality control within laboratory using standard laboratory test.

• During 2004 I worked for 6 months, at the molecular and clinical virology labs, on a clinical graduation thesis titled The HCV viral subtypes distributions in my region. I collected more than 1000 blood samples, detected Hcv IgG antibodies in human serum or plasma using M.E.I.A immunoassay, and evaluated, the concentration of virus with real-time P.C.R. system COBAS® AmpliPrep/COBAS® TaqMan® Hcv Test developed by Roche diagnostic, and viral genotypes throught RIBA TEST developed by Bayer, then I analyzed statistically all collected data.

• On 10/2004, participated in the refresher course Drug monitoring.

• On 112004, participated in the refresher course New molecular and clinical assays in microbiology.

09/1995-07/2000 High school qualification of expert technician (course of study in medical biology and chemistry) at the “Galileo Galilei” Industrial Technical High school Institute

COMPUTER SKILLS:

Excellent computer knowledge, about Microsoft Windows XP, 7, VISTA operating systems , OFFICE, excellent knowledge about internet, mail boxes, Adobe reader, writer, professional software , photo editing software and the ECDL CERTIFICATE, European computer driving licence.

ADDITIONAL PERSONAL INFORMATIONS:

• 02/2012 revisited G.R.E graduate record examination.

• 10/2011 TOEFL-iBT certificate

• 10/2003 University of Cambridge P.E.T certificate

• 05/2002 ECDL European computer driving licence

WORK EXPERIENCES:

03/2007/-12/2007 European institute of Oncology, Milan Italy

Pathological anatomy Unit, histopathology and cytopathology

• Specimen entering, data checking

• Routine and general Histological techniques including dissection, tissue processing, embedding, paraffin- and cryo-sectioning, HE staining and special staining

• Preparing reagents and solutions

• Immunohistochemistry, Immunocytochemistry and Enzyme histochemistry stainings

• Image acquisition, processing and analysis including light and fluorescent microscopies

• Routine cytological techniques, samples processing, staining, PAP TEST.

09/2006-03/2007 Pfizer, Milan Italy

• pharmacological surveillance (Safe and Risk Management) microbiology area

• data analyses

12/2004-07/2006 Santa Rita clinic, Milan Italy, central laboratory

Microbiology

• Bacteriological procedures like staining, culturing of clinical specimens

• Fluorescent staining, Acid fast staining and culturing of bacteria, Mycobacteria

• Serological procedures including MEIA, EIA,ELISA and other immunoenzymatic assays, Widal, VDRL, ASO, TPHA

• Enter all anaerobic, aerobic bloods bottles to machines.

• Fungal culture and detection of parasites from different specimens

• Antibiotic sensitivity testing

Hematology and coagulation

• coagulation test

• Performed, D-Dimer, PT, PTT, fibrinogen, QC, ACL, advanced instruments and maintenance to the instrument

• Worked in CBC coulter, ESR sedimentation rate, all CBC test.

• May-Grunwald Giemsa staining, microscope observation

• Reading all bloods, wounds

• Hematology duties included performing complete blood counts with cytohematology identification

Immunohematology:

• I performed Type and Screen, cross match, antibody identification and unit typing,

• Researched ABO, Rh and cross match for compatibility with patient and unit of blood for transfusion.

10/2011

Baldi Daniele

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