Scientist / Manager Scientist
Resume:
CURRICULUM VITAE
NAME: Ravi Kothapalli
PRESENT ADDRESS:
Residence: 29623 Birds eye Dr.
Wesley Chapel, FL.33543
Phone: 813-***-****
Phone: 813-***-****
Cell: 813-***-****
E.mail: ypp7of@r.postjobfree.com
EDUCATION:
Degree University
Bachelor of Science Andhra University, India
(Chemistry, Botany, & Zoology)
Master of Science Andhra University, India
(Biochemistry)
Ph. D. University of Western Ontario,
(Biochemistry) London, Ontario, Canada
ACCREDIDATION and NATIONAL CERTIFICATION
Technologist in Molecular Biology (ASCP), MB (ASCP) 2009
Clinical laboratory Supervisor (Molecular Pathology)
( Florida State) 2009
RELATED WORK EXPERIENCE:
July 2010- Present
Research Specialist
Tissue Core
Moffitt Cancer Center
Tampa, Florida, 33612.
August 30, 2007 to 2010
Courtesy Assistant Professor
Department of Chemical Engineering
Bio-medical Engineering
College of Engineering
University of South Florida
Tampa, FL-33620-5350
June 16, 2008 to 6/30/2009
Associate in Research
Center for Biological Defense
College of Public Health
University of South Florida
Experience gained: Molecular biology of influenza virus. Cloned all 8 segments of Influenza Virus ( H3N2 Wyoming) and sequenced the whole genome completely without any errors. Supervised the technicians and students in molecular biology. Transfected the viral segments into HEK 293 cell lines in order to study the expression of viral segments.
January 16, 2006 to June 14, 2008:
Laboratory Manager
Research Staff Scientist
Shriners Hospital for Children
12502 Pine Drive, Tampa, FL-33612
Experience gained: Supervised approximately 10 employees including research scientists, research technicians and students. Developed cell cultures for Osteoclast and Osteoblasts at Shriners hospital. Standardized and performed Real Time PCR for various genes involved in bone injury and bone repair. Extensive work has been performed with cartilage obtained from young and old pigs. Microarry studies were performed and identified several genes involved in bone repair.
August 1998 to – October 31, 2005
Research Assistant Professor
DIO, H. Lee Moffitt Cancer Center and
Department of Internal Medicine,
College of Medicine, University of South Florida,
Tampa, Florida, U.S.A
Experienced gained: Supervised approximately 10 employees including Research Technicians, Medical Residency Students and University of South Florida students. My work was mainly on LGL leukemia Patients. Isolating PBMC’s (Peripheral Blood Mononuclear Cells) from LGL leukemia patients and also normal individuals, then performing molecular biology studies. Isolating RNA, DNA, PCR, Cloning, Microarray, Northern Blots, Southern and Western blots. Novel genes involved the disease was identified and characterized.
October 1995 August 1998:
Research Associate
Department of Pathology, College of Medicine,
University of South Florida,
Tampa, Florida, U.S.A
Experienced gained: Worked with human endometrial tissue. Identified a novel gene EBAF by performing Differential Display Technique. Worked with several human cancer tissues to identify differentially expressed genes.
September 1993 September 1995:
Molecular Biologist
Forest Pest Management Institute
Sault Ste. Marie, Ontario, Canada
Experienced gained: Insect cell culture and majority of molecular biology techniques were learned.
October 1990--July 1993: Research Associate
Department of Biochemistry
University of Western Ontario
London, Ontario, Canada
October 1988 October 1990: Postdoctoral Fellow
Department of Biochemistry
University of Texas Health Science
Center, San Antonio, Texas, U.S.A
January 1986 October 1988: Lecturer, Department of Biochemistry
Andhra University, India
October 1985 December 1985: Postdoctoral Fellow, Department of Plant
Sciences, University of Western Ontario
London, Ontario, Canada
January 1982 December 1985: Graduate Student
University of Western Ontario
London, Ontario, Canada
July 1976 December 1981 Lecturer, Department of Biochemistry
Andhra University, India
Patents:
Nucleic acids encoding an endometrial bleeding associated factor (EBAF), United States Patent RE40,937 .
Issued on October 13, 2009
Sphingosine 1-Phosphate receptor gene, Sppr. US Patent No. 7,220, 580, issued on May 22, 2007.
Method for the diagnosis of selected adenocarcinomas. US Patent No. 5916751. Issued on June 29, 1999.
Differentially expressed genes in Large Granular Lymphocyte leukemia. Patent pending
Discovery of Alternative Splice Variants of Human Serine proteases. Patent pending
Farnesylamine derivatives as potential cell-growth inhibitors, anti-cancer and anti-viral agents. Patent pending
PUBLICATIONS:
1.Molecular profiling of LGL leukemia reveals role of sphingolipid signaling in survival of cytotoxic lymphocytes.Shah MV, Zhang R, Irby R, Kothapalli R, Liu X, Arrington T, Frank B, Lee NH, Loughran TP Jr.
Blood. 112,770-781. 2008.
2. Kothapalli R, Aya-Ay PJ, Bain H, Garces A, Kim HK. Ischaemic injury to femoral head induces apoptotic and oncotic cell death. Pathology, 39, 241-246, 2007.
3. Kothapalli R, Nyland SB, Kusmartseva I, Bailey RD, McKeown TM and Loughran TP Jr. Constitutive production of proinflammatory cytokines RANTES, MIP-1 and IL-18 characterizes LGL leukemia. Inter Natl J Oncol, 26, 529-535, 2005
4. Kothapalli R, Yoder SJ, Kusmartseva I, Loughran TP Jr. Nov 2003. Characterization of a variant of PAC-1 in large granular lymphocyte leukemia. Protein Expr Purif. 32:52-60
5. Kothapalli R, Danyluck GM, Bailey RD, Loughran TP Jr. May 2003. Problems associated with product enhancement reverse transcriptase assay using bacteriophage MS2 RNA as a template. Journal of Virological Methods. 109:203-7.
6. Ravi Kothapalli, Ratna D. Bailey, Irina Kusmartseva, Shrikant Mane, P.K. Epling-Burnette and Thomas P. Loughran Jr. Constitutive Expression of Cytotoxic Proteases and Down-regulation of Protease Inhibitors in LGL Leukemia, Inter Natl J. Oncol, 22, 33-39, 2003
7. Ravi Kothapalli, Irena Kusmartseva, Lisa Smith Thomas P. Loughran Jr. Characterization of a human sphingosine-1-phosphate receptor gene (S1P5) and its differential expression in LGL leukemia. Biochem. Biophys. Acta. 2002, 1579, 117-123.
8. Kothapalli R, Yoder SJ, Mane S and Thomas P. Loughran TP Jr. Microarray Results: How Accurate Are They ? BMC Bioinformatics. 3:22, 2002.
9. Epling-Burnette PK, Liu JH, Catlett-Falcone R, Turkson J, Oshiro M, Kothapalli R, Li Y, Wang JM, Yang-Yen HF, Karras J, Jove R, Loughran TP Jr. Inhibition of STAT3 signaling leads to apoptosis of leukemic large granular lymphocytes and decreased Mcl-1 expression. J Clin Invest. 107:351-362, 2001.
10. Palli SR, Kothapalli R, Feng Q, Perera SC, Zheng, SC, Gojtan K, Pang A, Primavera M, Ladd T, Tomkins W, Retnakaran A. Molecular analysis of overwintering diapause in Insect Timing: circadian rhythmicity to seasonality. Edited by D.L. Denlinger, J. Giebultowicz, and D.S. Saunders, Elsevier Science, Chapter 11. 2001.
11. Tabibzadeh S, Kothapalli R, Buyuksal I. Distinct tumor specific expression of TGFB4 (ebaf)*, a novel human gene of the TGF-beta superfamily Front Biosci. 2:a 18-25. 1997.
12.Kothapalli R, Buyuksal I, Wu SQ, Chegini N, Tabibzadeh S. Detection of ebaf, a novel human gene of the transforming growth factor beta superfamily association of gene expression with endometrial bleeding. J Clin Invest. 99:2342-2350, 1997.
13.Tabibzadeh S, Kothapalli R. From steroid signals to local regulatory factors involved in endometrial bleeding.Eur J Obstet Gynecol Reprod Biol. 70:25-27, 1996.
14.Carroll KK, Kothapalli R. Lui EMK , Guthrie N, and A F. Chambers.. Biological Effects of Long-Chain Fatty Amines. Malaysian Oil Science and Technology (MOST). 4: 124-125, 1995.
15. Kothapalli R, Palli SR, Ladd, T and Retnakaran, A. Cloning and developmental Expression of the ecdysone receptor gene from spruce budworm (Choristoneura fumiferna). Deve. Genetics., 17: 319-330, 1995.
16. Kothapalli R Lui EMK, Guthrie N, Chambers AF and Carroll KK. Effect of long-chain fattyamines on the growth of ras-transformed NIH 3T3 cells. Biochemical Pharmacology. 47: 1909-1916, 1994.
17. Kothapalli R, Guthrie N, Haines DS M and Carroll KK. Variation of serum levels of dolichol in relation to its occurrence in human atherosclerotic plaques. Biochem. Cell Biol. 72: 62-65, 1994.
18. Kothapalli R., Guthrie N, Chambers NF, Carroll KK. Farnesylamine: A potent inhibitor of farnesylation and growth of ras-transformed cells. Lipids. 28: 969-973, 1993.
19. Sprague EA, Kothapalli R, Karbacher JJ, Edwards H, Schwartz CJ and Elbein, AD. Inhibition of scavenger receptor-mediated modified LDL endocytosis in bovine aortic endothelial cells by glycoprotein processing inhibitor castanospermine. Biochemistry. 32: 8888-8895, 1993.
20. Carroll KK, Guthrie N, and Kothapalli R. Dolichol: Function, Metaboism and Accumulation in human tissues. Biochem. Cell Biol. 70:282-284, 1992.
21. Kothapalli R, Hu C, Reddi, PS. and van Huystee R.B. Effect of tunicamycin on peroxidase release by cultured peanut suspension cells. J. Exptl. Botany. 37:1708-1715, 1986.
22. Kothapalli R, Rip, JW. and Carroll KK. Submicrosomal distribution of dolichol kinase and dolichyl phosphate phosphatase in the microsomes of germinating soybeans. Biochim. Biophys. Acta. 884:497-501, 1986.
23. Ravi, Kothapalli., Rip, J.W. and Carroll, K.K. (1986) Regulation of dolichol and dolichyl phosphate during germination and early development of soybeans. Biochim. Biophys. Acta. 875:626-632.
24. Ravi, Kothapalli., Rip, J.W. and Carroll, K.K. (1986) Characterization of dolichol kinase from soybean seedling. Biochim. Biophys.Acta. 875:618-625.
25. Rip, J.W., Rupar, A.C., Ravi, Kothapalli and Carroll, K.K. (1985) Distribution, metabolism and function of dolichol and polyprenols. Prog. Lipid Res. 24:269-309.
26. Ravi, Kothapalli., Rip, J.W. and Carroll, K.K. (1984) Differences in polyisoprenoid alcohols of mono-and dicotyledonous seeds. Lipids 19:401-404.
27. Ravi, Kothapalli, Rip, J.W. and Carroll, K.K. (1983) Characterization of dolichol and dolichyl phosphate phosphatase from soyabeans (Glycine max). Biochem. J. 213:513-518.
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