Research Assistant

KHAJA WAHEEDUDDIN SYED

*** ***** ******

Jersey city, New Jersey

Cell no: 201-***-****

*******************@*****.***

EDUCATION

ARNOLD AND MARIE SCHWARTZ COLLEGE OF PHARMACY

Long Island University, Brooklyn, NY.

Start:September 06, 2007 to june 2009

Master of Sciences Degree-Pharmaceutical science with specialization in Industrial Pharmacy

PROFESSIONAL EXPERIENCE: (02/22/2008 to Present)

COLUMBIA UNIVERSITY MEDICAL CENTER

DEPARTMENT OF MICROBIOLOGY AND THE INSTITUTE OF CANCER RESEARCH (NEW YORK)

DEPARTMENT OF MICROBIOLOGY AND IMMUNOLOGY

• Research Assistant (Full time lab experience)

• Basic techniques of microbiology such as media preparation, mammalian cell culture, separation of cell colonies, transformation of bacteria , cloning and genotyping

• Delivery of plasmid DNA, siRNA, shRNA, using biodegradable nanoparticles on cell line such as mouse CD8+ and CD4+ cells and dendritic cells and bone marrow cells and human primary cell line CD8+ .

• Optimisation of size of FDA approved biodegradable poly lactide co glycolide nanoparticles.

SKILLS

Research: Finding the role of Cblb protein in breast cancer and Ablation of

Cblb protein to enhance tumour degradation

• Mini, midi, and maxi-plasmid extraction and purification

• Restriction

• Antibody extraction using bioreactor

• Design siRNA, shRNA, and pDNA sequence.

• Plasmid DNA cloning

• Transformation of E.coli bacteria

• In vitro transfection of Cell line

• In vitro dialysis

• Antibody conjugation

• Antibody Staining

• Isolation of T-cells from spleen of mouse

• Isolation of bone marrow cells from mouse and Growth of primary CD11c cells.

• Culture and growth of Human primary cell line and Mouse Dendritic Cells and bone marrow cells..

• Using Nanotechnology to target specific cd4+ and cd8+ cell line in mouse and human .

• Formulation of matrix control release tablet

• Quality control

• Designing of Mathematical models for pharmacokinetic data i.e blood and urine samples

INSTRUMENTAL ANALYSIS :

• Flow Cytometry

• Florescence Microscope

• Electron Microscopy

• PCR(Polymerase chain reaction)

• Antibody staining

• Antibody extraction using bioreactor

• ULTRA VIOLET SPECTROSCOPY

• Infra red spectroscopy

• PH calibration

• Incubator

• Asceptic Techniques

• Particle sizer and zeta sizer

• Homogeniser

• Dissolution Apparatus (USP 1&2)

• Rotary tablet press

• HPLC

• Preparation of Poly lactide co Glycolide Biodegradable Nanoparticles

• Malvern Sonicator

• Malvern zeta sizer for measuring size of nanoparticles

• GEL Electrophoresis

• Western blot for protein analysis

• Mean, Median, Standard deviation, Standard error

• WINNONLIN PROGRAMMING Mathematical models for pharmacokinetic data

Volunteer - MAHATMA GANDHI MEMORIAL HOSPITAL

• Dispensing drugs, Assisting in data entry, report relay(though the phone), specimen labeling and slide preparations as well as observing laboratory operations

work.(DECEMBER 2006 to JUNE 2007)

Blood Donations for Red cross society

Academic Reinforcement Center(ARC)

Tutoring one-on-one and small-group,

student tutorials in all areas of chemistry, Mathmatics and Biology. (February 07,2008 to June 2008)

Software:

• Windows 7, MS office-word, Excel, Power point, Adobe photo shop(Certified)

ACTIVITIES

• Teaching Assistant in Long Island University, Brooklyn, NY (September 2007-

December 2009)

HONORS

• Dean’s List, every semester 2007 to 2009

REFERENCES

Hua Gu, Ph.D.

Associate Professor of Microbiology & Immunology

Ph.D., University of Cologne. Lymphocyte development and differentiation

Columbia university of physicians and surgeons DEPARTMENT OF MICROBIOLOGY AND IMMUNLOGY

Phone: 212-***-****

Fax: 212-***-****

Email: ******@********.***

• WEB SITE : http://www.microbiology.columbia.edu/faculty/gu.html

Contact this candidate